| SILVA-PEDROSA, Rita, Jonas CAMPOS, Aline Marie FERNANDES, Miguel Joao FERREIRA DA SILVA, Carla CALCADA, Ana MAROTE, Olga MARTINHO, Maria Isabel VEIGA, Ligia R. RODRIGUES, Antonio Jose SALGADO and Pedro Eduardo FERREIRA. Cerebral Malaria Model Applying Human Brain Organoids. Cells. MDPI, 2023, vol. 12, No 7, p. 1-31. ISSN 2073-4409. Available from: https://dx.doi.org/10.3390/cells12070984. |
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@article{2301841,
author = {SilvaandPedrosa, Rita and Campos, Jonas and Fernandes, Aline Marie and Ferreira da Silva, Miguel Joao and Calcada, Carla and Marote, Ana and Martinho, Olga and Veiga, Maria Isabel and Rodrigues, Ligia R. and Salgado, Antonio Jose and Ferreira, Pedro Eduardo},
article_number = {7},
doi = {http://dx.doi.org/10.3390/cells12070984},
keywords = {cerebral malaria; brain organoids; transcriptome; human iPSCs; secretome; HBMEC activation},
language = {eng},
issn = {2073-4409},
journal = {Cells},
title = {Cerebral Malaria Model Applying Human Brain Organoids},
url = {https://doi.org/10.3390/cells12070984},
volume = {12},
year = {2023}
}
TY - JOUR
ID - 2301841
AU - Silva-Pedrosa, Rita - Campos, Jonas - Fernandes, Aline Marie - Ferreira da Silva, Miguel Joao - Calcada, Carla - Marote, Ana - Martinho, Olga - Veiga, Maria Isabel - Rodrigues, Ligia R. - Salgado, Antonio Jose - Ferreira, Pedro Eduardo
PY - 2023
TI - Cerebral Malaria Model Applying Human Brain Organoids
JF - Cells
VL - 12
IS - 7
SP - 1-31
EP - 1-31
PB - MDPI
SN - 20734409
KW - cerebral malaria
KW - brain organoids
KW - transcriptome
KW - human iPSCs
KW - secretome
KW - HBMEC activation
UR - https://doi.org/10.3390/cells12070984
N2 - Neural injuries in cerebral malaria patients are a significant cause of morbidity and mortality. Nevertheless, a comprehensive research approach to study this issue is lacking, so herein we propose an in vitro system to study human cerebral malaria using cellular approaches. Our first goal was to establish a cellular system to identify the molecular alterations in human brain vasculature cells that resemble the blood-brain barrier (BBB) in cerebral malaria (CM). Through transcriptomic analysis, we characterized specific gene expression profiles in human brain microvascular endothelial cells (HBMEC) activated by the Plasmodium falciparum parasites. We also suggest potential new genes related to parasitic activation. Then, we studied its impact at brain level after Plasmodium falciparum endothelial activation to gain a deeper understanding of the physiological mechanisms underlying CM. For that, the impact of HBMEC-P. falciparum-activated secretomes was evaluated in human brain organoids. Our results support the reliability of in vitro cellular models developed to mimic CM in several aspects. These systems can be of extreme importance to investigate the factors (parasitological and host) influencing CM, contributing to a molecular understanding of pathogenesis, brain injury, and dysfunction.
ER -
SILVA-PEDROSA, Rita, Jonas CAMPOS, Aline Marie FERNANDES, Miguel Joao FERREIRA DA SILVA, Carla CALCADA, Ana MAROTE, Olga MARTINHO, Maria Isabel VEIGA, Ligia R. RODRIGUES, Antonio Jose SALGADO and Pedro Eduardo FERREIRA. Cerebral Malaria Model Applying Human Brain Organoids. \textit{Cells}. MDPI, 2023, vol.~12, No~7, p.~1-31. ISSN~2073-4409. Available from: https://dx.doi.org/10.3390/cells12070984.
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