Enrichment of human nasopharyngeal bacteriome with bacteria
from dust after short-term exposure to indoor environment: a
pilot study

J 2023

Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study

KONEČNÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ et. al.

Basic information

Original name

Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study

Authors

KONEČNÁ, Eva (203 Czech Republic, belonging to the institution), Petra VÍDEŇSKÁ (203 Czech Republic, belonging to the institution), Lucie BUREŠOVÁ (203 Czech Republic, belonging to the institution), Milan URÍK (703 Slovakia, belonging to the institution), Soňa SMETANOVÁ (203 Czech Republic, belonging to the institution), Stanislav SMATANA (703 Slovakia, belonging to the institution), Roman PROKEŠ (203 Czech Republic, belonging to the institution), Barbara LÁNÍČKOVÁ (203 Czech Republic, belonging to the institution), Eva BUDINSKÁ (703 Slovakia, belonging to the institution), Jana KLÁNOVÁ (203 Czech Republic, belonging to the institution) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

BMC Microbiology, London, BioMed Central, 2023, 1471-2180

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.200 in 2022

RIV identification code

RIV/00216224:14310/23:00131440

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1186/s12866-023-02951-5

UT WoS

001040951200002

Keywords in English

Bacteriome; Dust; Nasopharynx; Household; Hospital; Sequencing; 16S rRNA; Exposure; Indoor environment

Abstract

V originále

BackgroundIndoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments.MethodsIn this pilot study, nasopharyngeal swabs were taken from 22 participants in the morning and after 8 h of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants' households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital - NEO, 6 from a pediatric hospital - ENT, and 8 from a research center - RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices.ResultsStaphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 h of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76% of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These "enriching" bacterial genera overlapped between the hospital workplaces - NEO and ENT but differed from those in the research center - RCX.ConclusionsThe results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day. Short-term exposure to the indoor environment can result in the enrichment of the nasopharynx with bacterial DNA from indoor dust; the bacterial composition, however, varies by the indoor workplace environment.

Links

EF15_003/0000469, research and development project

Name: Cetocoen Plus

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

EF19_073/0016943, research and development project

Name: Interní grantová agentura Masarykovy univerzity

GA22-32743S, research and development project

Name: Propojení složení chemických látek a mikrobiomu v domácím prachu s environmentálními parametry

Investor: Czech Science Foundation

LM2018132, research and development project

Name: Národní centrum lékařské genomiky (Acronym: NCLG)

Investor: Ministry of Education, Youth and Sports of the CR, National Center for Medical Genomics

LM2023069, research and development project

Name: Výzkumná infrastruktura RECETOX

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX research infrastructure

Files attached

2302818.pdf

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Citovat

KONEČNÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ, Stanislav SMATANA, Roman PROKEŠ, Barbara LÁNÍČKOVÁ, Eva BUDINSKÁ, Jana KLÁNOVÁ and Petra BOŘILOVÁ LINHARTOVÁ. Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study. BMC Microbiology. London: BioMed Central, 2023, vol. 23, No 1, p. 1-16. ISSN 1471-2180. Available from: https://dx.doi.org/10.1186/s12866-023-02951-5.

@article{2302818,
   author = {Konečná, Eva and Vídeňská, Petra and Burešová, Lucie and Urík, Milan and Smetanová, Soňa and Smatana, Stanislav and Prokeš, Roman and Láníčková, Barbara and Budinská, Eva and Klánová, Jana and Bořilová Linhartová, Petra},
   article_location = {London},
   article_number = {1},
   doi = {http://dx.doi.org/10.1186/s12866-023-02951-5},
   keywords = {Bacteriome; Dust; Nasopharynx; Household; Hospital; Sequencing; 16S rRNA; Exposure; Indoor environment},
   language = {eng},
   issn = {1471-2180},
   journal = {BMC Microbiology},
   title = {Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study},
   url = {https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-02951-5},
   volume = {23},
   year = {2023}
}

TY  - JOUR
ID  - 2302818
AU  - Konečná, Eva - Vídeňská, Petra - Burešová, Lucie - Urík, Milan - Smetanová, Soňa - Smatana, Stanislav - Prokeš, Roman - Láníčková, Barbara - Budinská, Eva - Klánová, Jana - Bořilová Linhartová, Petra
PY  - 2023
TI  - Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study
JF  - BMC Microbiology
VL  - 23
IS  - 1
SP  - 1-16
EP  - 1-16
PB  - BioMed Central
SN  - 14712180
KW  - Bacteriome
KW  - Dust
KW  - Nasopharynx
KW  - Household
KW  - Hospital
KW  - Sequencing
KW  - 16S rRNA
KW  - Exposure
KW  - Indoor environment
UR  - https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-02951-5
N2  - BackgroundIndoor dust particles are an everyday source of human exposure to microorganisms and their inhalation may directly affect the microbiota of the respiratory tract. We aimed to characterize the changes in human nasopharyngeal bacteriome after short-term exposure to indoor (workplace) environments.MethodsIn this pilot study, nasopharyngeal swabs were taken from 22 participants in the morning and after 8 h of their presence at the workplace. At the same time points, indoor dust samples were collected from the participants' households (16 from flats and 6 from houses) and workplaces (8 from a maternity hospital - NEO, 6 from a pediatric hospital - ENT, and 8 from a research center - RCX). 16S rRNA sequencing analysis was performed on these human and environmental matrices.ResultsStaphylococcus and Corynebacterium were the most abundant genera in both indoor dust and nasopharyngeal samples. The analysis indicated lower bacterial diversity in indoor dust samples from flats compared to houses, NEO, ENT, and RCX (p < 0.05). Participants working in the NEO had the highest nasopharyngeal bacterial diversity of all groups (p < 0.05). After 8 h of exposure to the workplace environment, enrichment of the nasopharynx with several new bacterial genera present in the indoor dust was observed in 76% of study participants; however, no significant changes were observed at the level of the nasopharyngeal bacterial diversity (p > 0.05, Shannon index). These "enriching" bacterial genera overlapped between the hospital workplaces - NEO and ENT but differed from those in the research center - RCX.ConclusionsThe results suggest that although the composition of nasopharyngeal bacteriome is relatively stable during the day. Short-term exposure to the indoor environment can result in the enrichment of the nasopharynx with bacterial DNA from indoor dust; the bacterial composition, however, varies by the indoor workplace environment.
ER  -

KONEČNÁ, Eva, Petra VÍDEŇSKÁ, Lucie BUREŠOVÁ, Milan URÍK, Soňa SMETANOVÁ, Stanislav SMATANA, Roman PROKEŠ, Barbara LÁNÍČKOVÁ, Eva BUDINSKÁ, Jana KLÁNOVÁ and Petra BOŘILOVÁ LINHARTOVÁ. Enrichment of human nasopharyngeal bacteriome with bacteria from dust after short-term exposure to indoor environment: a pilot study. \textit{BMC Microbiology}. London: BioMed Central, 2023, vol.~23, No~1, p.~1-16. ISSN~1471-2180. Available from: https://dx.doi.org/10.1186/s12866-023-02951-5.

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