Feasibility of laser-induced breakdown spectroscopy to
elucidate elemental changes in human tooth ankylosis

J 2023

Feasibility of laser-induced breakdown spectroscopy to elucidate elemental changes in human tooth ankylosis

POŘÍZKA, Pavel, Anna KONEČNÁ, Anna ŠINDELÁŘOVÁ, Marie ŠULCOVÁ, Pavlína MODLITBOVÁ et. al.

Basic information

Original name

Feasibility of laser-induced breakdown spectroscopy to elucidate elemental changes in human tooth ankylosis

Authors

POŘÍZKA, Pavel (203 Czech Republic), Anna KONEČNÁ (203 Czech Republic, guarantor), Anna ŠINDELÁŘOVÁ (203 Czech Republic), Marie ŠULCOVÁ (203 Czech Republic, belonging to the institution), Pavlína MODLITBOVÁ (203 Czech Republic), David PROCHAZKA (203 Czech Republic), Petra NEVORÁNKOVÁ (203 Czech Republic, belonging to the institution), Michal NAVRÁTIL (203 Czech Republic, belonging to the institution), Lucie VRLÍKOVÁ (203 Czech Republic), Marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution) and Jozef KAISER (203 Czech Republic)

Edition

Spectrochimica Acta Part B: Atomic Spectroscopy, OXFORD, Elsevier, 2023, 0584-8547

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30208 Dentistry, oral surgery and medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.300 in 2022

RIV identification code

RIV/00216224:14310/23:00131533

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.sab.2023.106727

UT WoS

001143779300001

Keywords in English

Ankylosis; Bioimaging; Human tooth; Bone; Laser-induced breakdown spectroscopy

Abstract

V originále

Tooth ankylosis is a pathological condition associated with the loss of physiological tooth mobility when the firm fusion between the alveolar bone and the tooth root occurs. Causes of dental ankylosis are uncertain, so the analysis of elemental distribution in ankylotic and surrounding tissues could provide additional information about its initiation and progression. Here, we used Laser-Induced Breakdown Spectroscopy (LIBS) to determine differences in the mineral composition among ankylotic tissue, bone, and dental tissue taking advantage of its high throughput and multi-elemental capability. Elemental imaging was performed with a spatial resolution of 30 μm to evaluate the distribution of carbon, calcium, magnesium, phosphorus, and strontium in human tooth. To further verify the difference in the mineral composition of ankylotic tissue, the semi-quantitative content of these elements was compared within the region of interest. We revealed a significant increase in calcium, magnesium, and phosphorus contents in the ankylotic tissues. However, the contents of magnesium and phosphorus were not significantly changed. This finding suggests a mineral disbalance only of just certain elements in the tooth-bone interface area during the spread of ankylosis associated with an intense calcification of connective tissue. This paper brings a feasibility study and shows the way of LIBS data interpretation. We propose that the LIBS analysis on a micro-scale can contribute to the understanding of ankylotic tissue composition and can distinguish even small differences of carbon, calcium, magnesium, phosphorus, and strontium contents on the tooth-bone boundary during the initiation of ankylosis. Therefore, it represents a new useful tool for their future, more extensive analyses.

Citovat

POŘÍZKA, Pavel, Anna KONEČNÁ, Anna ŠINDELÁŘOVÁ, Marie ŠULCOVÁ, Pavlína MODLITBOVÁ, David PROCHAZKA, Petra NEVORÁNKOVÁ, Michal NAVRÁTIL, Lucie VRLÍKOVÁ, Marcela BUCHTOVÁ and Jozef KAISER. Feasibility of laser-induced breakdown spectroscopy to elucidate elemental changes in human tooth ankylosis. Spectrochimica Acta Part B: Atomic Spectroscopy. OXFORD: Elsevier, 2023, vol. 206, August 2023, p. 1-8. ISSN 0584-8547. Available from: https://dx.doi.org/10.1016/j.sab.2023.106727.

@article{2304918,
   author = {Pořízka, Pavel and Konečná, Anna and Šindelářová, Anna and Šulcová, Marie and Modlitbová, Pavlína and Prochazka, David and Nevoránková, Petra and Navrátil, Michal and Vrlíková, Lucie and Buchtová, Marcela and Kaiser, Jozef},
   article_location = {OXFORD},
   article_number = {August 2023},
   doi = {http://dx.doi.org/10.1016/j.sab.2023.106727},
   keywords = {Ankylosis; Bioimaging; Human tooth; Bone; Laser-induced breakdown spectroscopy},
   language = {eng},
   issn = {0584-8547},
   journal = {Spectrochimica Acta Part B: Atomic Spectroscopy},
   title = {Feasibility of laser-induced breakdown spectroscopy to elucidate elemental changes in human tooth ankylosis},
   url = {https://doi.org/10.1016/j.sab.2023.106727},
   volume = {206},
   year = {2023}
}

TY  - JOUR
ID  - 2304918
AU  - Pořízka, Pavel - Konečná, Anna - Šindelářová, Anna - Šulcová, Marie - Modlitbová, Pavlína - Prochazka, David - Nevoránková, Petra - Navrátil, Michal - Vrlíková, Lucie - Buchtová, Marcela - Kaiser, Jozef
PY  - 2023
TI  - Feasibility of laser-induced breakdown spectroscopy to elucidate elemental changes in human tooth ankylosis
JF  - Spectrochimica Acta Part B: Atomic Spectroscopy
VL  - 206
IS  - August 2023
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 05848547
KW  - Ankylosis
KW  - Bioimaging
KW  - Human tooth
KW  - Bone
KW  - Laser-induced breakdown spectroscopy
UR  - https://doi.org/10.1016/j.sab.2023.106727
N2  - Tooth ankylosis is a pathological condition associated with the loss of physiological tooth mobility when the firm fusion between the alveolar bone and the tooth root occurs. Causes of dental ankylosis are uncertain, so the analysis of elemental distribution in ankylotic and surrounding tissues could provide additional information about its initiation and progression. Here, we used Laser-Induced Breakdown Spectroscopy (LIBS) to determine differences in the mineral composition among ankylotic tissue, bone, and dental tissue taking advantage of its high throughput and multi-elemental capability. Elemental imaging was performed with a spatial resolution of 30 μm to evaluate the distribution of carbon, calcium, magnesium, phosphorus, and strontium in human tooth. To further verify the difference in the mineral composition of ankylotic tissue, the semi-quantitative content of these elements was compared within the region of interest. We revealed a significant increase in calcium, magnesium, and phosphorus contents in the ankylotic tissues. However, the contents of magnesium and phosphorus were not significantly changed. This finding suggests a mineral disbalance only of just certain elements in the tooth-bone interface area during the spread of ankylosis associated with an intense calcification of connective tissue. This paper brings a feasibility study and shows the way of LIBS data interpretation. We propose that the LIBS analysis on a micro-scale can contribute to the understanding of ankylotic tissue composition and can distinguish even small differences of carbon, calcium, magnesium, phosphorus, and strontium contents on the tooth-bone boundary during the initiation of ankylosis. Therefore, it represents a new useful tool for their future, more extensive analyses.
ER  -

POŘÍZKA, Pavel, Anna KONEČNÁ, Anna ŠINDELÁŘOVÁ, Marie ŠULCOVÁ, Pavlína MODLITBOVÁ, David PROCHAZKA, Petra NEVORÁNKOVÁ, Michal NAVRÁTIL, Lucie VRLÍKOVÁ, Marcela BUCHTOVÁ and Jozef KAISER. Feasibility of laser-induced breakdown spectroscopy to elucidate elemental changes in human tooth ankylosis. \textit{Spectrochimica Acta Part B: Atomic Spectroscopy}. OXFORD: Elsevier, 2023, vol.~206, August 2023, p.~1-8. ISSN~0584-8547. Available from: https://dx.doi.org/10.1016/j.sab.2023.106727.

Detailed Information on Publication Record