Spatial positioning of preimplantation mouse embryo cells is
regulated by mTORC1 and m(7)G-cap-dependent translation at the
8-to 16-cell transition

J 2023

Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition

GAHUROVÁ, Lenka, Jana TOMÁNKOVÁ, Pavla ČERNÁ, Pablo BORA, Michaela KUBÍČKOVÁ et. al.

Basic information

Original name

Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition

Authors

GAHUROVÁ, Lenka (203 Czech Republic), Jana TOMÁNKOVÁ (203 Czech Republic), Pavla ČERNÁ (203 Czech Republic), Pablo BORA (203 Czech Republic), Michaela KUBÍČKOVÁ (203 Czech Republic), Giorgio VIRNICCHI (203 Czech Republic), Kristina KOVAŘOVICOVÁ, David POTĚŠIL (203 Czech Republic, belonging to the institution), Pavel HRUŠKA (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Martin ANGER, Andrej ŠUŠOT (203 Czech Republic) and Alexander W. BRUCE (203 Czech Republic, guarantor)

Edition

OPEN BIOLOGY, ENGLAND, ROYAL SOC, 2023, 2046-2441

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 5.800 in 2022

RIV identification code

RIV/00216224:14740/23:00131564

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1098/rsob.230081

UT WoS

001044130500002

Keywords in English

mTOR;mTORC1;EIF4EBP1;4EBP1;TOP-motif;preimplantation mouse embryo;cell fate;inner cell mass;ICM and cell positioning

Abstract

V originále

Preimplantation mouse embryo development involves temporal-spatial specification and segregation of three blastocyst cell lineages: trophectoderm, primitive endoderm and epiblast. Spatial separation of the outer-trophectoderm lineage from the two other inner-cell-mass (ICM) lineages starts with the 8- to 16-cell transition and concludes at the 32-cell stages. Accordingly, the ICM is derived from primary and secondary contributed cells; with debated relative EPI versus PrE potencies. We report generation of primary but not secondary ICM populations is highly dependent on temporal activation of mammalian target of Rapamycin (mTOR) during 8-cell stage M-phase entry, mediated via regulation of the 7-methylguanosine-cap (m(7)G-cap)-binding initiation complex (EIF4F) and linked to translation of mRNAs containing 5 & PRIME; UTR terminal oligopyrimidine (TOP-) sequence motifs, as knockdown of identified TOP-like motif transcripts impairs generation of primary ICM founders. However, mTOR inhibition-induced ICM cell number deficits in early blastocysts can be compensated by the late blastocyst stage, after inhibitor withdrawal; compensation likely initiated at the 32-cell stage when supernumerary outer cells exhibit molecular characteristics of inner cells. These data identify a novel mechanism specifically governing initial spatial segregation of mouse embryo blastomeres, that is distinct from those directing subsequent inner cell formation, contributing to germane segregation of late blastocyst lineages.

Links

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

LM2023042, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministry of Education, Youth and Sports of the CR, CIISB - Czech Infrastructure for Integrative Structural Biology

90242, large research infrastructures

Name: CIISB III

Citovat

GAHUROVÁ, Lenka, Jana TOMÁNKOVÁ, Pavla ČERNÁ, Pablo BORA, Michaela KUBÍČKOVÁ, Giorgio VIRNICCHI, Kristina KOVAŘOVICOVÁ, David POTĚŠIL, Pavel HRUŠKA, Zbyněk ZDRÁHAL, Martin ANGER, Andrej ŠUŠOT and Alexander W. BRUCE. Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition. OPEN BIOLOGY. ENGLAND: ROYAL SOC, 2023, vol. 13, No 8, p. 1-19. ISSN 2046-2441. Available from: https://dx.doi.org/10.1098/rsob.230081.

@article{2305479,
   author = {Gahurová, Lenka and Tománková, Jana and Černá, Pavla and Bora, Pablo and Kubíčková, Michaela and Virnicchi, Giorgio and Kovařovicová, Kristina and Potěšil, David and Hruška, Pavel and Zdráhal, Zbyněk and Anger, Martin and Šušot, Andrej and Bruce, Alexander W.},
   article_location = {ENGLAND},
   article_number = {8},
   doi = {http://dx.doi.org/10.1098/rsob.230081},
   keywords = {mTOR;mTORC1;EIF4EBP1;4EBP1;TOP-motif;preimplantation mouse embryo;cell fate;inner cell mass;ICM and cell positioning},
   language = {eng},
   issn = {2046-2441},
   journal = {OPEN BIOLOGY},
   title = {Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition},
   url = {https://royalsocietypublishing.org/doi/epdf/10.1098/rsob.230081},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2305479
AU  - Gahurová, Lenka - Tománková, Jana - Černá, Pavla - Bora, Pablo - Kubíčková, Michaela - Virnicchi, Giorgio - Kovařovicová, Kristina - Potěšil, David - Hruška, Pavel - Zdráhal, Zbyněk - Anger, Martin - Šušot, Andrej - Bruce, Alexander W.
PY  - 2023
TI  - Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition
JF  - OPEN BIOLOGY
VL  - 13
IS  - 8
SP  - 1-19
EP  - 1-19
PB  - ROYAL SOC
SN  - 20462441
KW  - mTOR;mTORC1;EIF4EBP1;4EBP1;TOP-motif;preimplantation mouse embryo;cell fate;inner cell mass;ICM and cell positioning
UR  - https://royalsocietypublishing.org/doi/epdf/10.1098/rsob.230081
N2  - Preimplantation mouse embryo development involves temporal-spatial specification and segregation of three blastocyst cell lineages: trophectoderm, primitive endoderm and epiblast. Spatial separation of the outer-trophectoderm lineage from the two other inner-cell-mass (ICM) lineages starts with the 8- to 16-cell transition and concludes at the 32-cell stages. Accordingly, the ICM is derived from primary and secondary contributed cells; with debated relative EPI versus PrE potencies. We report generation of primary but not secondary ICM populations is highly dependent on temporal activation of mammalian target of Rapamycin (mTOR) during 8-cell stage M-phase entry, mediated via regulation of the 7-methylguanosine-cap (m(7)G-cap)-binding initiation complex (EIF4F) and linked to translation of mRNAs containing 5 & PRIME; UTR terminal oligopyrimidine (TOP-) sequence motifs, as knockdown of identified TOP-like motif transcripts impairs generation of primary ICM founders. However, mTOR inhibition-induced ICM cell number deficits in early blastocysts can be compensated by the late blastocyst stage, after inhibitor withdrawal; compensation likely initiated at the 32-cell stage when supernumerary outer cells exhibit molecular characteristics of inner cells. These data identify a novel mechanism specifically governing initial spatial segregation of mouse embryo blastomeres, that is distinct from those directing subsequent inner cell formation, contributing to germane segregation of late blastocyst lineages.
ER  -

GAHUROVÁ, Lenka, Jana TOMÁNKOVÁ, Pavla ČERNÁ, Pablo BORA, Michaela KUBÍČKOVÁ, Giorgio VIRNICCHI, Kristina KOVAŘOVICOVÁ, David POTĚŠIL, Pavel HRUŠKA, Zbyněk ZDRÁHAL, Martin ANGER, Andrej ŠUŠOT and Alexander W. BRUCE. Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and m(7)G-cap-dependent translation at the 8-to 16-cell transition. \textit{OPEN BIOLOGY}. ENGLAND: ROYAL SOC, 2023, vol.~13, No~8, p.~1-19. ISSN~2046-2441. Available from: https://dx.doi.org/10.1098/rsob.230081.

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