Characteristics of N-Glycosylation and Its Impact on the
Molecular Behavior of Lupinus angustifolius ?-Conglutin

J 2023

Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin

CZUBINSKI, Jaroslaw, Erika LATTOVÁ, Zbyněk ZDRÁHAL and Richard STRASSER

Basic information

Original name

Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin

Authors

CZUBINSKI, Jaroslaw (616 Poland), Erika LATTOVÁ (703 Slovakia, guarantor, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Richard STRASSER (40 Austria)

Edition

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, UNITED STATES, AMER CHEMICAL SOC, 2023, 0021-8561

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40500 4.5 Other agricultural sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 6.100 in 2022

RIV identification code

RIV/00216224:14740/23:00131565

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/acs.jafc.3c00727

UT WoS

000986537100001

Keywords in English

lupin seed;gamma-conglutin;N-glycosylation;mass spectrometry;post-translational modification;thermal stability

Abstract

V originále

a-Conglutin, a lupin seed protein, is an intriguing protein both in terms of the complexity of its molecular structure and a broad spectrum of unique health-promoting properties manifested in animal and human trials. Moreover, this protein is an evolutionary cornerstone whose physiological significance for the plant has not been determined yet. Herein, a comprehensive characterization of a-conglutin glycosylation is presented and includes the identification of the N-glycan-bearing site, the qualitative and quantitative composition of glycan-building saccharides, as well as the effect of oligosaccharide removal on structural and thermal stability. The obtained results indicate the presence of glycans belonging to different classes attached to the Asn98 residue. In addition, the detachment of the oligosaccharide significantly affects secondary structure composition, which disturbs the oligomerization process. The structural changes were also reflected in biophysical parameters, i.e., at a pH value of 4.5, an increase in a-conglutin thermal stability was observed for the deglycosylated monomeric form. Collectively, the presented results provide evidence of the high complexity of the post-translational maturation and suggest the possibility of a functional effect that glycosylation might have on a-conglutin structure integrity.

Links

EF16_026/0008446, research and development project

Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

90242, large research infrastructures

Name: CIISB III

Citovat

CZUBINSKI, Jaroslaw, Erika LATTOVÁ, Zbyněk ZDRÁHAL and Richard STRASSER. Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. UNITED STATES: AMER CHEMICAL SOC, 2023, vol. 71, No 19, p. 7359-7369. ISSN 0021-8561. Available from: https://dx.doi.org/10.1021/acs.jafc.3c00727.

@article{2305480,
   author = {Czubinski, Jaroslaw and Lattová, Erika and Zdráhal, Zbyněk and Strasser, Richard},
   article_location = {UNITED STATES},
   article_number = {19},
   doi = {http://dx.doi.org/10.1021/acs.jafc.3c00727},
   keywords = {lupin seed;gamma-conglutin;N-glycosylation;mass spectrometry;post-translational modification;thermal stability},
   language = {eng},
   issn = {0021-8561},
   journal = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
   title = {Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin},
   url = {https://pubs.acs.org/doi/epdf/10.1021/acs.jafc.3c00727},
   volume = {71},
   year = {2023}
}

TY  - JOUR
ID  - 2305480
AU  - Czubinski, Jaroslaw - Lattová, Erika - Zdráhal, Zbyněk - Strasser, Richard
PY  - 2023
TI  - Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin
JF  - JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
VL  - 71
IS  - 19
SP  - 7359-7369
EP  - 7359-7369
PB  - AMER CHEMICAL SOC
SN  - 00218561
KW  - lupin seed;gamma-conglutin;N-glycosylation;mass spectrometry;post-translational modification;thermal stability
UR  - https://pubs.acs.org/doi/epdf/10.1021/acs.jafc.3c00727
N2  - a-Conglutin, a lupin seed protein, is an intriguing protein both in terms of the complexity of its molecular structure and a broad spectrum of unique health-promoting properties manifested in animal and human trials. Moreover, this protein is an evolutionary cornerstone whose physiological significance for the plant has not been determined yet. Herein, a comprehensive characterization of a-conglutin glycosylation is presented and includes the identification of the N-glycan-bearing site, the qualitative and quantitative composition of glycan-building saccharides, as well as the effect of oligosaccharide removal on structural and thermal stability. The obtained results indicate the presence of glycans belonging to different classes attached to the Asn98 residue. In addition, the detachment of the oligosaccharide significantly affects secondary structure composition, which disturbs the oligomerization process. The structural changes were also reflected in biophysical parameters, i.e., at a pH value of 4.5, an increase in a-conglutin thermal stability was observed for the deglycosylated monomeric form. Collectively, the presented results provide evidence of the high complexity of the post-translational maturation and suggest the possibility of a functional effect that glycosylation might have on a-conglutin structure integrity.
ER  -

CZUBINSKI, Jaroslaw, Erika LATTOVÁ, Zbyněk ZDRÁHAL and Richard STRASSER. Characteristics of N-Glycosylation and Its Impact on the Molecular Behavior of Lupinus angustifolius ?-Conglutin. \textit{JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}. UNITED STATES: AMER CHEMICAL SOC, 2023, vol.~71, No~19, p.~7359-7369. ISSN~0021-8561. Available from: https://dx.doi.org/10.1021/acs.jafc.3c00727.

Detailed Information on Publication Record