Light-responsive microRNA molecules in human retinal organoids
are differentially regulated by distinct wavelengths of light

J 2023

Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light

ÇELIKER, Canan, Kamila WEISSOVÁ, Kateřina AMRUZ ČERNÁ, Jan OPPELT, Birthe DORGAU et. al.

Basic information

Original name

Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light

Authors

ÇELIKER, Canan (792 Turkey, belonging to the institution), Kamila WEISSOVÁ (203 Czech Republic, belonging to the institution), Kateřina AMRUZ ČERNÁ (203 Czech Republic, belonging to the institution), Jan OPPELT (203 Czech Republic), Birthe DORGAU, Francisco MOLINA GAMBIN (724 Spain, belonging to the institution), Jana ŠEBESTÍKOVÁ (203 Czech Republic, belonging to the institution), Majlinda LAKO, Evelyne SERNAGOR, Petra LISKOVA (203 Czech Republic) and Tomáš BÁRTA (203 Czech Republic, guarantor, belonging to the institution)

Edition

iSCIENCE, CAMBRIDGE, CELL PRESS, 2023, 2589-0042

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.800 in 2022

RIV identification code

RIV/00216224:14110/23:00131593

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.isci.2023.107237

UT WoS

001049218100001

Keywords in English

Light-responsive microRNA molecules; human retinal organoids

Abstract

V originále

Cells in the human retina must rapidly adapt to constantly changing visual stimuli. This fast adaptation to varying levels and wavelengths of light helps to regulate circadian rhythms and allows for adaptation to high levels of illumination, thereby enabling the rest of the visual system to remain responsive. It has been shown that retinal microRNA (miRNA) molecules play a key role in regulating these processes. However, despite extensive research using various model organisms, light-regulated miRNAs in human retinal cells remain unknown. Here, we aim to characterize these miRNAs. We generated light-responsive human retinal organoids that express miRNA families and clusters typically found in the retina. Using an in-house developed photostimulation device, we identified a subset of light regulated miRNAs. Importantly, we found that these miRNAs are differentially regulated by distinct wavelengths of light and have a rapid turnover, highlighting the dynamic and adaptive nature of the human retina.

Links

GA21-08182S, research and development project

Name: Retinální organoidy: Zkoumání účinků fotostimulace na lidskou sítnici

Investor: Czech Science Foundation

LM2023050, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR, Czech BioImaging: National research infrastructure for biological and medical imaging

LM2023067, research and development project

Name: Národní centrum lékařské genomiky

Investor: Ministry of Education, Youth and Sports of the CR, NCMG: The National Centre for Medical Genomic

NU22-07-00380, research and development project

Name: Aplikace retinálních buněk a organoidů ve funkční diagnostice a léčbě ztráty zraku u Bardet-Biedlova syndromu

Investor: Ministry of Health of the CR, Subprogram 1 - standard

90250, large research infrastructures

Name: Czech-BioImaging III

90267, large research infrastructures

Name: NCMG III

Citovat

ÇELIKER, Canan, Kamila WEISSOVÁ, Kateřina AMRUZ ČERNÁ, Jan OPPELT, Birthe DORGAU, Francisco MOLINA GAMBIN, Jana ŠEBESTÍKOVÁ, Majlinda LAKO, Evelyne SERNAGOR, Petra LISKOVA and Tomáš BÁRTA. Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light. iSCIENCE. CAMBRIDGE: CELL PRESS, 2023, vol. 26, No 7, p. 1-20. ISSN 2589-0042. Available from: https://dx.doi.org/10.1016/j.isci.2023.107237.

@article{2306618,
   author = {Çeliker, Canan and Weissová, Kamila and Amruz Černá, Kateřina and Oppelt, Jan and Dorgau, Birthe and Molina Gambin, Francisco and Šebestíková, Jana and Lako, Majlinda and Sernagor, Evelyne and Liskova, Petra and Bárta, Tomáš},
   article_location = {CAMBRIDGE},
   article_number = {7},
   doi = {http://dx.doi.org/10.1016/j.isci.2023.107237},
   keywords = {Light-responsive microRNA molecules; human retinal organoids},
   language = {eng},
   issn = {2589-0042},
   journal = {iSCIENCE},
   title = {Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light},
   url = {https://www.sciencedirect.com/science/article/pii/S2589004223013147?via%3Dihub},
   volume = {26},
   year = {2023}
}

TY  - JOUR
ID  - 2306618
AU  - Çeliker, Canan - Weissová, Kamila - Amruz Černá, Kateřina - Oppelt, Jan - Dorgau, Birthe - Molina Gambin, Francisco - Šebestíková, Jana - Lako, Majlinda - Sernagor, Evelyne - Liskova, Petra - Bárta, Tomáš
PY  - 2023
TI  - Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light
JF  - iSCIENCE
VL  - 26
IS  - 7
SP  - 1-20
EP  - 1-20
PB  - CELL PRESS
SN  - 25890042
KW  - Light-responsive microRNA molecules
KW  - human retinal organoids
UR  - https://www.sciencedirect.com/science/article/pii/S2589004223013147?via%3Dihub
N2  - Cells in the human retina must rapidly adapt to constantly changing visual stimuli. This fast adaptation to varying levels and wavelengths of light helps to regulate circadian rhythms and allows for adaptation to high levels of illumination, thereby enabling the rest of the visual system to remain responsive. It has been shown that retinal microRNA (miRNA) molecules play a key role in regulating these processes. However, despite extensive research using various model organisms, light-regulated miRNAs in human retinal cells remain unknown. Here, we aim to characterize these miRNAs. We generated light-responsive human retinal organoids that express miRNA families and clusters typically found in the retina. Using an in-house developed photostimulation device, we identified a subset of light regulated miRNAs. Importantly, we found that these miRNAs are differentially regulated by distinct wavelengths of light and have a rapid turnover, highlighting the dynamic and adaptive nature of the human retina.
ER  -

$\backslash$C CELIKER, Canan, Kamila WEISSOVÁ, Kateřina AMRUZ ČERNÁ, Jan OPPELT, Birthe DORGAU, Francisco MOLINA GAMBIN, Jana ŠEBESTÍKOVÁ, Majlinda LAKO, Evelyne SERNAGOR, Petra LISKOVA and Tomáš BÁRTA. Light-responsive microRNA molecules in human retinal organoids are differentially regulated by distinct wavelengths of light. \textit{iSCIENCE}. CAMBRIDGE: CELL PRESS, 2023, vol.~26, No~7, p.~1-20. ISSN~2589-0042. Available from: https://dx.doi.org/10.1016/j.isci.2023.107237.

Detailed Information on Publication Record