Autonomous capillary electrophoresis processing and analysis of
dried blood spots for high-throughput determination of uric
acid

J 2023

Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid

MORAVČÍK, Ondrej, Miloš DVOŘÁK and Pavel KUBÁŇ

Basic information

Original name

Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid

Authors

MORAVČÍK, Ondrej (703 Slovakia, belonging to the institution), Miloš DVOŘÁK and Pavel KUBÁŇ (guarantor)

Edition

Analytica Chimica Acta, Amsterdam, Elsevier, 2023, 0003-2670

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.200 in 2022

RIV identification code

RIV/00216224:14310/23:00131620

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.aca.2023.341390

UT WoS

001002501500001

Keywords in English

Automation; Dried blood spot; Capillary electrophoresis; Uric acid

Abstract

V originále

A new set-up for fully autonomous and high-throughput capillary electrophoresis (CE) analyses of dried blood spot (DBS) samples is presented. The DBS samples were prepared by collecting exactly 5 μL of capillary blood from a finger-prick onto a pre-punched DBS disc in a disposable plastic CE vial and by in-vial blood drying. The vials with the DBS samples were then loaded into a commercial CE instrument for a fully unmanned sample processing and analysis. A fused-silica capillary of the CE instrument was first used for the transfer of 100 μL of elution solvent to each vial, in-vial DBS elution, and in-vial eluate homogenization. The same capillary was also used for at-line injection, separation, and selective analysis of the resulting eluates. Novel CE sequences were tailor-programmed for consecutive processing and analyses of multiple DBSs, which facilitated a fully autonomous determination of uric acid with a throughput of 240 DBS samples per day (24 h). The presented analytical protocol (using 100 μm i. d./30 cm capillary; 30 mM 2-(N-morpholino)-ethanesulfonic acid, 30 mM l-histidine, and 30 μM cetyltrimethylammonium bromide background electrolyte solution; and UV detection at 292 nm) provided excellent precision at endogenous and spiked uric acid concentrations with RSD values of peak areas below 3.2%. Calibration curves were linear over the 33.3 − 1200 μM range (R2 better than 0.998), limits of detection and quantification in the original capillary blood were 10 and 33.3 μM, respectively, and were well below the uric acid clinical range (140–420 μM). The stability of uric acid in DBS samples stored at laboratory temperature for up to 2 months was also excellent demonstrating less than a 4.2% decrease in uric acid concentrations. The actual set-up might thus be highly attractive for clinical subjects and laboratories because it is minimally invasive and requires minimum intervention from laboratory staff.

Citovat

MORAVČÍK, Ondrej, Miloš DVOŘÁK and Pavel KUBÁŇ. Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid. Analytica Chimica Acta. Amsterdam: Elsevier, 2023, vol. 1267, August, p. 1-8. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2023.341390.

@article{2307637,
   author = {Moravčík, Ondrej and Dvořák, Miloš and Kubáň, Pavel},
   article_location = {Amsterdam},
   article_number = {August},
   doi = {http://dx.doi.org/10.1016/j.aca.2023.341390},
   keywords = {Automation; Dried blood spot; Capillary electrophoresis; Uric acid},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid},
   url = {https://doi.org/10.1016/j.aca.2023.341390},
   volume = {1267},
   year = {2023}
}

TY  - JOUR
ID  - 2307637
AU  - Moravčík, Ondrej - Dvořák, Miloš - Kubáň, Pavel
PY  - 2023
TI  - Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid
JF  - Analytica Chimica Acta
VL  - 1267
IS  - August
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 00032670
KW  - Automation
KW  - Dried blood spot
KW  - Capillary electrophoresis
KW  - Uric acid
UR  - https://doi.org/10.1016/j.aca.2023.341390
N2  - A new set-up for fully autonomous and high-throughput capillary electrophoresis (CE) analyses of dried blood spot (DBS) samples is presented. The DBS samples were prepared by collecting exactly 5 μL of capillary blood from a finger-prick onto a pre-punched DBS disc in a disposable plastic CE vial and by in-vial blood drying. The vials with the DBS samples were then loaded into a commercial CE instrument for a fully unmanned sample processing and analysis. A fused-silica capillary of the CE instrument was first used for the transfer of 100 μL of elution solvent to each vial, in-vial DBS elution, and in-vial eluate homogenization. The same capillary was also used for at-line injection, separation, and selective analysis of the resulting eluates. Novel CE sequences were tailor-programmed for consecutive processing and analyses of multiple DBSs, which facilitated a fully autonomous determination of uric acid with a throughput of 240 DBS samples per day (24 h). The presented analytical protocol (using 100 μm i. d./30 cm capillary; 30 mM 2-(N-morpholino)-ethanesulfonic acid, 30 mM l-histidine, and 30 μM cetyltrimethylammonium bromide background electrolyte solution; and UV detection at 292 nm) provided excellent precision at endogenous and spiked uric acid concentrations with RSD values of peak areas below 3.2%. Calibration curves were linear over the 33.3 − 1200 μM range (R2 better than 0.998), limits of detection and quantification in the original capillary blood were 10 and 33.3 μM, respectively, and were well below the uric acid clinical range (140–420 μM). The stability of uric acid in DBS samples stored at laboratory temperature for up to 2 months was also excellent demonstrating less than a 4.2% decrease in uric acid concentrations. The actual set-up might thus be highly attractive for clinical subjects and laboratories because it is minimally invasive and requires minimum intervention from laboratory staff.
ER  -

MORAVČÍK, Ondrej, Miloš DVOŘÁK and Pavel KUBÁŇ. Autonomous capillary electrophoresis processing and analysis of dried blood spots for high-throughput determination of uric acid. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier, 2023, vol.~1267, August, p.~1-8. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2023.341390.

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