a 2023

Vitronectin is a xeno-free and defined surface for the derivation of human embryonic stem cells

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Michal JEŠETA, Pavel VENTRUBA, Aleš HAMPL et. al.

Basic information

Original name

Vitronectin is a xeno-free and defined surface for the derivation of human embryonic stem cells

Authors

SOURALOVÁ, Tereza (203 Czech Republic, belonging to the institution), Daniela HULÍNOVÁ (203 Czech Republic, belonging to the institution), Michal JEŠETA (203 Czech Republic, belonging to the institution), Pavel VENTRUBA (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Irena KOUTNÁ (203 Czech Republic, belonging to the institution)

Edition

ISSCR Annual meeting 2023, Boston, USA, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10605 Developmental biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/23:00131628

Organization unit

Faculty of Medicine

Keywords in English

human embryonic stem cells; Vitronectin

Tags

International impact, Reviewed
Změněno: 10/4/2024 13:05, Mgr. Tereza Miškechová

Abstract

V originále

Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, diseasemodelling, and drug development. Their derivation is usually performed using a feeder layer, which isundefined and potentially contagious, and because of these difficulties, there is a tendency to replacefeeders with xeno-free defined substrates in recent years. We used truncated vitronectin for thederivation of hESCs for the first time, derived three hESC lines, and confirmed their undifferentiatedstate, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers. These results support the conclusion that truncated vitronectin is a defined xeno-freesubstrate that is suitable for the derivation of hESCs.

Links

MUNI/A/1301/2022, interní kód MU
Name: Zdroje pro tkáňové inženýrství 13
Investor: Masaryk University
MUNI/IGA/1332/2021, interní kód MU
Name: The impact of derivation substrate on differentiation of human embryonic stem cells for the cell therapy
Investor: Masaryk University
NU22-08-00629, research and development project
Name: Vývoj postupů pro léčbu makulární degenerace sítnice deriváty lidských pluripotentních kmenových buněk
Investor: Ministry of Health of the CR, Subprogram 1 - standard