Detailed Information on Publication Record
2023
Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
CHOCHOLOVÁ, Eva, Pavel ROUDNICKÝ, David POTĚŠIL, Dana FIALOVÁ, Karolína KRYŠTOFOVÁ et. al.Basic information
Original name
Extraction Protocol for Parallel Analysis of Proteins and DNA from Ancient Teeth and Dental Calculus
Authors
CHOCHOLOVÁ, Eva (203 Czech Republic, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Dana FIALOVÁ (203 Czech Republic, belonging to the institution), Karolína KRYŠTOFOVÁ (203 Czech Republic, belonging to the institution), Eva DROZDOVÁ (203 Czech Republic, belonging to the institution) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution)
Edition
Journal of Proteome Research, American Chemical Society, 2023, 1535-3893
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.400 in 2022
RIV identification code
RIV/00216224:14740/23:00131731
Organization unit
Central European Institute of Technology
UT WoS
001067227500001
Keywords in English
bioarcheology; paleoproteomics; ancientDNA; ancientproteins; dental calculus
Tags
International impact, Reviewed
Změněno: 2/11/2024 20:13, Ing. Martina Blahová
Abstract
V originále
Dental calculus is becoming a crucial material in the study of past populations with increasing interest in its proteomic and genomic content. Here, we suggest further development of a protocol for analysis of ancient proteins and a combined approach for subsequent ancient DNA extraction. We tested the protocol on recent teeth, and the optimized protocol was applied to ancient tooth to limit the destruction of calculus as it is a precious and irreplaceable source of dietary, microbiological, and ecological information in the archeological context. Finally, the applicability of the protocol was demonstrated on samples of the ancient calculus.
Links
| EF18_046/0015974, research and development project |
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| LM2018127, research and development project |
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| LM2018140, research and development project |
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| LM2023042, research and development project |
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| MUNI/A/1325/2021, interní kód MU |
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| MUNI/A/1522/2020, interní kód MU |
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