Detailed Information on Publication Record
2023
Palaeoproteomic and Genomic Analyses of Bronze Age Population from Unětice Culture, Moravia, Czech Republic
CHOCHOLOVÁ, Eva, Anna ŠENOVSKÁ, Pavel ROUDNICKÝ, Zbyněk ZDRÁHAL, Kristýna BRZOBOHATÁ et. al.Basic information
Original name
Palaeoproteomic and Genomic Analyses of Bronze Age Population from Unětice Culture, Moravia, Czech Republic
Authors
CHOCHOLOVÁ, Eva (203 Czech Republic, guarantor, belonging to the institution), Anna ŠENOVSKÁ (203 Czech Republic, belonging to the institution), Pavel ROUDNICKÝ (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Kristýna BRZOBOHATÁ (203 Czech Republic, belonging to the institution), Dana FIALOVÁ (203 Czech Republic, belonging to the institution), Petr KOS (203 Czech Republic) and Eva DROZDOVÁ (203 Czech Republic, belonging to the institution)
Edition
10th Meeting of the International Society for Biomolecular Archaeology, New Horizons in Biomolecular Archaeology, 2023
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10608 Biochemistry and molecular biology
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/23:00131733
Organization unit
Faculty of Science
Keywords (in Czech)
Metagenomika; 16S rRNA; paleoproteomika; starobylá DNA; aDNA; doba bronzová; mitogenom; Únětice
Keywords in English
Metagenomics; 16S rRNA; palaeoproteomics; ancient DNA; aDNA; Bronze Age; Mitogenome; Únětice
Tags
International impact, Reviewed
Změněno: 19/9/2023 15:47, Mgr. Eva Chocholová
Abstract
V originále
Mitogenome research offers an intriguing insight into the history, origin, contacts, and variability of past populations and has become a standard part of the study of past populations. Ancient dental calculus is also a well-known and valuable source of information that is used to investigate health, diet, migrations, or habits in the context of biomolecular archaeology. The studied individuals were excavated at a Bronze Age site of Brno-Tuřany, Moravia region, Czech Republic. They belonged to the Unětice culture, dated to 2300-1600 BC. For the present study, we decided to combine both approaches: genomic analysis of mitochondrial DNA in 16 individuals and proteomic analysis of dental calculus in 8 individuals. Sampling, decontamination, ancient DNA extraction, library preparation, and the first stages of protein extraction were carried out in the specialised facility for ancient biomolecules in the Laboratory of Biological and Molecular Anthropology. DNA was purified using silica columns and the whole mitogenome libraries were prepared according to Šenovská et al. (2021) with mtDNA capture. The proteins were extracted with the FASP protocol modified for ancient biomolecules. LC-MS/MS analysis was performed on timsTOF Pro combined with nanoElute (Bruker). Based on mtDNA, the population from Brno-Tuřany was very variable and six general haplogroups were found: N (1), W (1), H (4), V (2), K (1), and the most frequent U (7). No shared haplotype occurred between the studied individuals. The identified dietary proteins originated from, e.g. Bos sp., Ovis/Capra sp., Triticum aestivum or Brassica sp. The biomolecular approach in archaeology therefore offers new insights into past populations.
Links
| LM2015091, research and development project |
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| LM2018140, research and development project |
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| LM2023042, research and development project |
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| MUNI/A/1325/2021, interní kód MU |
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