J 2023

Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus rutilus), Common Bream (Abramis brama) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids?

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea, Kristína KŘÍŽOVÁ, Kristýna VOŘÍŠKOVÁ, Lukáš VETEŠNÍK, Vojtěch BYSTRÝ et. al.

Basic information

Original name

Transcriptome Profile Analyses of Head Kidney in Roach (Rutilus rutilus), Common Bream (Abramis brama) and Their Hybrids: Does Infection by Monogenean Parasites in Freshwater Fish Reveal Differences in Fish Vigour among Parental Species and Their Hybrids?

Authors

VETEŠNÍKOVÁ ŠIMKOVÁ, Andrea (203 Czech Republic, guarantor, belonging to the institution), Kristína KŘÍŽOVÁ (703 Slovakia, belonging to the institution), Kristýna VOŘÍŠKOVÁ (203 Czech Republic, belonging to the institution), Lukáš VETEŠNÍK (203 Czech Republic, belonging to the institution), Vojtěch BYSTRÝ (203 Czech Republic, belonging to the institution) and Martin DEMKO (703 Slovakia, belonging to the institution)

Edition

Biology, Basel, MDPI, 2023, 2079-7737

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 4.200 in 2022

RIV identification code

RIV/00216224:14310/23:00131742

Organization unit

Faculty of Science

DOI

UT WoS

001076917100001

Keywords in English

Monogenea; Paradiplozoon homoion; freshwater fish; hybridization; RNA seq; differential gene expression; hybrid heterosis; hybrid breakdown

Tags

Tags

International impact, Reviewed
Změněno: 8/4/2024 10:34, Mgr. Eva Dubská

Abstract

V originále

Interspecific hybrids of F1 generations have frequently been characterized by high vigour resulting from heterosis advantage. In contrast, post-F1 generations are expected to express hybrid breakdown, i.e., they suffer from low viability and survival, reproductive abnormalities or sterility and limited ecological performance. Resistance or susceptibility to parasites is one of the measures reflecting hybrid vigour. The present study aimed to analyse the experimental infection of the blood-feeding generalist parasite Paradiplozoon homoion (Monogenea) in two target fish species, Abramis brama and Rutilus rutilus, and their reciprocal F1 hybrids and backcross hybrids, and to reveal potential parasite-induced changes in their transcriptome profiles of head kidney. We hypothesized various effects of hybridization in terms of parasitism in F1 hybrids and backcross hybrids reflected by differential gene expression. The number of differentially expressed genes (DEGs) differed between fish lines with a lower number of DEGs in F1 hybrids and a higher number in backcross hybrids when compared to the parental lines, A. brama and R. rutilus. Backcross hybrids were more infected than F1 hybrids and parental lines. DEG analyses revealed the role of heme binding, erythrocyte differentiation and immunity-related processes in fish after infection by blood-feeding P. homoion. Using GO and KEGG analyses, we revealed the similarity in DEGs between two backcross generations of hybrids. This finding may indicate a potential consequence of hybrid breakdown in backcross generations. Gene expression in less parasitized F1 hybrids is in line of hybrid advantage.

Links

GA19-10088S, research and development project
Name: Genetická disrupce u mezidruhových hybridů kaprovitých ryb: její dopad na kondici-asociované znaky, imunitu a parazitární zátěž
Investor: Czech Science Foundation
90267, large research infrastructures
Name: NCMG III