Identification of staphyloxanthin and derivates in
yellow-pigmented Staphylococcus capitis subsp. capitis

J 2023

Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis

KATHARINA, Siems, Runzheimer KATHARINA, Katarína REBROŠOVÁ, Etzbach LARA, Auerhammer ALINA et. al.

Basic information

Original name

Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis

Authors

KATHARINA, Siems, Runzheimer KATHARINA, Katarína REBROŠOVÁ (703 Slovakia, belonging to the institution), Etzbach LARA, Auerhammer ALINA, Rehm ANNA, Schwengers OLIVER, Martin ŠILER (203 Czech Republic), Ota SAMEK (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Moeller RALF

Edition

Frontiers in Microbiology, LAUSANNE, FRONTIERS MEDIA SA, 2023, 1664-302X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.200 in 2022

RIV identification code

RIV/00216224:14110/23:00131818

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3389/fmicb.2023.1272734

UT WoS

001085828700001

Keywords in English

Staphylococcus capitis; staphyloxanthin; coagulase-negative staphylococci (CoNS); carotenoids; bacterial pigments

Abstract

V originále

Introduction: Staphylococcus capitis naturally colonizes the human skin but as an opportunistic pathogen, it can also cause biofilm-associated infections and bloodstream infections in newborns. Previously, we found that two strains from the subspecies S. capitis subsp. capitis produce yellow carotenoids despite the initial species description, reporting this subspecies as non-pigmented. In Staphylococcus aureus, the golden pigment staphyloxanthin is an important virulence factor, protecting cells against reactive oxygen species and modulating membrane fluidity. Methods: In this study, we used two pigmented (DSM 111179 and DSM 113836) and two non-pigmented S. capitis subsp. capitis strains (DSM 20326T and DSM 31028) to identify the pigment, determine conditions under which pigment-production occurs and investigate whether pigmented strains show increased resistance to ROS and temperature stress. Results: We found that the non-pigmented strains remained colorless regardless of the type of medium, whereas intensity of pigmentation in the two pigmented strains increased under low nutrient conditions and with longer incubation times. We were able to detect and identify staphyloxanthin and its derivates in the two pigmented strains but found that methanol cell extracts from all four strains showed ROS scavenging activity regardless of staphyloxanthin production. Increased survival to cold temperatures (−20°C) was detected in the two pigmented strains only after long-term storage compared to the non-pigmented strains. Conclusion: The identification of staphyloxanthin in S. capitis is of clinical relevance and could be used, in the same way as in S. aureus, as a possible target for anti-virulence drug design.

Links

MUNI/A/1361/2022, interní kód MU

Name: Diagnostika a terapie původců nozokomiálních infekcí

Investor: Masaryk University, Diagnosis and therapy of agents causing nosocomial infections

NU21-05-00341, research and development project

Name: Pokročilé metody detekce patogenních mikroorganizmů a hodnocení účinku antimikrobiálních látek Ramanovou spektroskopií a dalšími metodami na úrovni molekul v optofluidních systémech

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

KATHARINA, Siems, Runzheimer KATHARINA, Katarína REBROŠOVÁ, Etzbach LARA, Auerhammer ALINA, Rehm ANNA, Schwengers OLIVER, Martin ŠILER, Ota SAMEK, Filip RŮŽIČKA and Moeller RALF. Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis. Frontiers in Microbiology. LAUSANNE: FRONTIERS MEDIA SA, 2023, vol. 14, September 2023, p. 1-12. ISSN 1664-302X. Available from: https://dx.doi.org/10.3389/fmicb.2023.1272734.

@article{2319558,
   author = {Katharina, Siems and Katharina, Runzheimer and Rebrošová, Katarína and Lara, Etzbach and Alina, Auerhammer and Anna, Rehm and Oliver, Schwengers and Šiler, Martin and Samek, Ota and Růžička, Filip and Ralf, Moeller},
   article_location = {LAUSANNE},
   article_number = {September 2023},
   doi = {http://dx.doi.org/10.3389/fmicb.2023.1272734},
   keywords = {Staphylococcus capitis; staphyloxanthin; coagulase-negative staphylococci (CoNS); carotenoids; bacterial pigments},
   language = {eng},
   issn = {1664-302X},
   journal = {Frontiers in Microbiology},
   title = {Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis},
   url = {https://www.frontiersin.org/articles/10.3389/fmicb.2023.1272734/full},
   volume = {14},
   year = {2023}
}

TY  - JOUR
ID  - 2319558
AU  - Katharina, Siems - Katharina, Runzheimer - Rebrošová, Katarína - Lara, Etzbach - Alina, Auerhammer - Anna, Rehm - Oliver, Schwengers - Šiler, Martin - Samek, Ota - Růžička, Filip - Ralf, Moeller
PY  - 2023
TI  - Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis
JF  - Frontiers in Microbiology
VL  - 14
IS  - September 2023
SP  - 1-12
EP  - 1-12
PB  - FRONTIERS MEDIA SA
SN  - 1664302X
KW  - Staphylococcus capitis
KW  - staphyloxanthin
KW  - coagulase-negative staphylococci (CoNS)
KW  - carotenoids
KW  - bacterial pigments
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2023.1272734/full
N2  - Introduction: Staphylococcus capitis naturally colonizes the human skin but as an opportunistic pathogen, it can also cause biofilm-associated infections and bloodstream infections in newborns. Previously, we found that two strains from the subspecies S. capitis subsp. capitis produce yellow carotenoids despite the initial species description, reporting this subspecies as non-pigmented. In Staphylococcus aureus, the golden pigment staphyloxanthin is an important virulence factor, protecting cells against reactive oxygen species and modulating membrane fluidity. Methods: In this study, we used two pigmented (DSM 111179 and DSM 113836) and two non-pigmented S. capitis subsp. capitis strains (DSM 20326T and DSM 31028) to identify the pigment, determine conditions under which pigment-production occurs and investigate whether pigmented strains show increased resistance to ROS and temperature stress. Results: We found that the non-pigmented strains remained colorless regardless of the type of medium, whereas intensity of pigmentation in the two pigmented strains increased under low nutrient conditions and with longer incubation times. We were able to detect and identify staphyloxanthin and its derivates in the two pigmented strains but found that methanol cell extracts from all four strains showed ROS scavenging activity regardless of staphyloxanthin production. Increased survival to cold temperatures (−20°C) was detected in the two pigmented strains only after long-term storage compared to the non-pigmented strains. Conclusion: The identification of staphyloxanthin in S. capitis is of clinical relevance and could be used, in the same way as in S. aureus, as a possible target for anti-virulence drug design.
ER  -

KATHARINA, Siems, Runzheimer KATHARINA, Katarína REBROŠOVÁ, Etzbach LARA, Auerhammer ALINA, Rehm ANNA, Schwengers OLIVER, Martin ŠILER, Ota SAMEK, Filip RŮŽIČKA and Moeller RALF. Identification of staphyloxanthin and derivates in yellow-pigmented Staphylococcus capitis subsp. capitis. \textit{Frontiers in Microbiology}. LAUSANNE: FRONTIERS MEDIA SA, 2023, vol.~14, September 2023, p.~1-12. ISSN~1664-302X. Available from: https://dx.doi.org/10.3389/fmicb.2023.1272734.

Detailed Information on Publication Record