Quantum Sensing for Detection of Zinc-Triggered Free Radicals
in Endothelial Cells

J 2023

Quantum Sensing for Detection of Zinc-Triggered Free Radicals in Endothelial Cells

WOJTAS, Daniel, Runrun LI, Anna JARZEBSKA, Bartosz SULKOWSKI, Michael ZEHETBAUER et. al.

Basic information

Original name

Quantum Sensing for Detection of Zinc-Triggered Free Radicals in Endothelial Cells

Authors

WOJTAS, Daniel (616 Poland, belonging to the institution), Runrun LI, Anna JARZEBSKA, Bartosz SULKOWSKI, Michael ZEHETBAUER, Erhard SCHAFLER, Krzysztof WIERZBANOWSKI, Aldona MZYK and Romana SCHIRHAGL (guarantor)

Edition

ADVANCED QUANTUM TECHNOLOGIES, Hoboken, WILEY, 2023, 2511-9044

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.400 in 2022

RIV identification code

RIV/00216224:14110/23:00131819

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/qute.202300174

UT WoS

001064838800001

Keywords in English

biodegradable zinc; diamond magnetometry; free radicals; NV centers; relaxometry

Abstract

V originále

Oxidative stress originating from the overproduction of free radicals poses a major threat to cell fate, therefore it is of great importance to address the formation of free radicals in cells subjected to various pathological stimuli. Here we investigate the free radical response of endothelial cells to biodegradable zinc. In addition to the standard free radical assays, relaxometry was used for determining the production of free radicals in cells exposed to non-physiological concentrations of zinc ions. The cellular morphology, intracellular zinc accumulation, as well as the levels of reactive oxygen/nitrogen species, are determined using standard fluorescent methods. For endothelial cells subjected to 50% zinc extracts, deviations from the normal cell shape and cell agglomeration tendency are observed. The culture medium containing the highest amount of zinc ions caused nuclei fragmentation, blebbing, and cell shrinkage, indicating cell death. A potential explanation for the observed phenomena is an overproduction of free radicals. In the case of 1% and 10% zinc extracts, the formation of free radicals is clearly confirmed by relaxometry, while the results obtained by using fluorescent techniques are unambiguous. It is revealed that high concentrations of zinc ions released from biodegradable samples induce a deleterious effect on endothelial cells.

Citovat

WOJTAS, Daniel, Runrun LI, Anna JARZEBSKA, Bartosz SULKOWSKI, Michael ZEHETBAUER, Erhard SCHAFLER, Krzysztof WIERZBANOWSKI, Aldona MZYK and Romana SCHIRHAGL. Quantum Sensing for Detection of Zinc-Triggered Free Radicals in Endothelial Cells. ADVANCED QUANTUM TECHNOLOGIES. Hoboken: WILEY, 2023, vol. 6, No 11, p. 1-13. ISSN 2511-9044. Available from: https://dx.doi.org/10.1002/qute.202300174.

@article{2319559,
   author = {Wojtas, Daniel and Li, Runrun and Jarzebska, Anna and Sulkowski, Bartosz and Zehetbauer, Michael and Schafler, Erhard and Wierzbanowski, Krzysztof and Mzyk, Aldona and Schirhagl, Romana},
   article_location = {Hoboken},
   article_number = {11},
   doi = {http://dx.doi.org/10.1002/qute.202300174},
   keywords = {biodegradable zinc; diamond magnetometry; free radicals; NV centers; relaxometry},
   language = {eng},
   issn = {2511-9044},
   journal = {ADVANCED QUANTUM TECHNOLOGIES},
   title = {Quantum Sensing for Detection of Zinc-Triggered Free Radicals in Endothelial Cells},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/qute.202300174},
   volume = {6},
   year = {2023}
}

TY  - JOUR
ID  - 2319559
AU  - Wojtas, Daniel - Li, Runrun - Jarzebska, Anna - Sulkowski, Bartosz - Zehetbauer, Michael - Schafler, Erhard - Wierzbanowski, Krzysztof - Mzyk, Aldona - Schirhagl, Romana
PY  - 2023
TI  - Quantum Sensing for Detection of Zinc-Triggered Free Radicals in Endothelial Cells
JF  - ADVANCED QUANTUM TECHNOLOGIES
VL  - 6
IS  - 11
SP  - 1-13
EP  - 1-13
PB  - WILEY
SN  - 25119044
KW  - biodegradable zinc
KW  - diamond magnetometry
KW  - free radicals
KW  - NV centers
KW  - relaxometry
UR  - https://onlinelibrary.wiley.com/doi/10.1002/qute.202300174
N2  - Oxidative stress originating from the overproduction of free radicals poses a major threat to cell fate, therefore it is of great importance to address the formation of free radicals in cells subjected to various pathological stimuli. Here we investigate the free radical response of endothelial cells to biodegradable zinc. In addition to the standard free radical assays, relaxometry was used for determining the production of free radicals in cells exposed to non-physiological concentrations of zinc ions. The cellular morphology, intracellular zinc accumulation, as well as the levels of reactive oxygen/nitrogen species, are determined using standard fluorescent methods. For endothelial cells subjected to 50% zinc extracts, deviations from the normal cell shape and cell agglomeration tendency are observed. The culture medium containing the highest amount of zinc ions caused nuclei fragmentation, blebbing, and cell shrinkage, indicating cell death. A potential explanation for the observed phenomena is an overproduction of free radicals. In the case of 1% and 10% zinc extracts, the formation of free radicals is clearly confirmed by relaxometry, while the results obtained by using fluorescent techniques are unambiguous. It is revealed that high concentrations of zinc ions released from biodegradable samples induce a deleterious effect on endothelial cells.
ER  -

WOJTAS, Daniel, Runrun LI, Anna JARZEBSKA, Bartosz SULKOWSKI, Michael ZEHETBAUER, Erhard SCHAFLER, Krzysztof WIERZBANOWSKI, Aldona MZYK and Romana SCHIRHAGL. Quantum Sensing for Detection of Zinc-Triggered Free Radicals in Endothelial Cells. \textit{ADVANCED QUANTUM TECHNOLOGIES}. Hoboken: WILEY, 2023, vol.~6, No~11, p.~1-13. ISSN~2511-9044. Available from: https://dx.doi.org/10.1002/qute.202300174.

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