Truncated vitronectin with E-cadherin enables the xeno-free
derivation of human embryonic stem cells

J 2023

Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Michal JEŠETA, Pavel VENTRUBA, Aleš HAMPL et. al.

Basic information

Original name

Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells

Authors

SOURALOVÁ, Tereza (203 Czech Republic, belonging to the institution), Daniela HULÍNOVÁ (203 Czech Republic, belonging to the institution), Michal JEŠETA (203 Czech Republic, belonging to the institution), Pavel VENTRUBA (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Irena KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific Reports, BERLIN, NATURE PORTFOLIO, 2023, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600 in 2022

RIV identification code

RIV/00216224:14110/23:00131861

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-023-42236-5

UT WoS

001067966700037

Keywords in English

vitronectin; E-cadherin; human embryonic stem cells

Abstract

V originále

Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, disease modeling, and drug development. Their derivation is usually performed using a feeder layer, which is undefined and can potentially cause a contamination by xeno components, therefore there is a tendency to replace feeders with xeno-free defined substrates in recent years. Three hESC lines were successfully derived on the vitronectin with a truncated N-terminus (VTN-N) in combination with E-cadherin in xeno-free conditions for the first time, and their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers were confirmed. These results support the conclusion that the VTN-N/E-cadherin is a suitable substrate for the xeno-free derivation of hESCs and can be used for the derivation of hESCs according to good manufacturing practices.

Links

EF19_073/0016943, research and development project

Name: Interní grantová agentura Masarykovy univerzity

MUNI/A/1301/2022, interní kód MU

Name: Zdroje pro tkáňové inženýrství 13

Investor: Masaryk University

MUNI/IGA/1332/2021, interní kód MU

Name: The impact of derivation substrate on differentiation of human embryonic stem cells for the cell therapy

Investor: Masaryk University

NU22-08-00629, research and development project

Name: Vývoj postupů pro léčbu makulární degenerace sítnice deriváty lidských pluripotentních kmenových buněk

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Michal JEŠETA, Pavel VENTRUBA, Aleš HAMPL and Irena KOUTNÁ. Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells. Scientific Reports. BERLIN: NATURE PORTFOLIO, 2023, vol. 13, No 1, p. 1-9. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-023-42236-5.

@article{2322797,
   author = {Souralová, Tereza and Hulínová, Daniela and Ješeta, Michal and Ventruba, Pavel and Hampl, Aleš and Koutná, Irena},
   article_location = {BERLIN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-023-42236-5},
   keywords = {vitronectin; E-cadherin; human embryonic stem cells},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells},
   url = {https://www.nature.com/articles/s41598-023-42236-5},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2322797
AU  - Souralová, Tereza - Hulínová, Daniela - Ješeta, Michal - Ventruba, Pavel - Hampl, Aleš - Koutná, Irena
PY  - 2023
TI  - Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
JF  - Scientific Reports
VL  - 13
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - NATURE PORTFOLIO
SN  - 20452322
KW  - vitronectin
KW  - E-cadherin
KW  - human embryonic stem cells
UR  - https://www.nature.com/articles/s41598-023-42236-5
N2  - Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, disease modeling, and drug development. Their derivation is usually performed using a feeder layer, which is undefined and can potentially cause a contamination by xeno components, therefore there is a tendency to replace feeders with xeno-free defined substrates in recent years. Three hESC lines were successfully derived on the vitronectin with a truncated N-terminus (VTN-N) in combination with E-cadherin in xeno-free conditions for the first time, and their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers were confirmed. These results support the conclusion that the VTN-N/E-cadherin is a suitable substrate for the xeno-free derivation of hESCs and can be used for the derivation of hESCs according to good manufacturing practices.
ER  -

SOURALOVÁ, Tereza, Daniela HULÍNOVÁ, Michal JEŠETA, Pavel VENTRUBA, Aleš HAMPL and Irena KOUTNÁ. Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells. \textit{Scientific Reports}. BERLIN: NATURE PORTFOLIO, 2023, vol.~13, No~1, p.~1-9. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-023-42236-5.

Detailed Information on Publication Record