Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry

ŠMAK, Pavel, Daniela FAČKOVCOVÁ, Katarína KOSTOLANSKÁ, Jana GREGOROVÁ, Eliška BARTEČKOVÁ, Jan JUŘICA, Libor USTOHAL, Jana HOŘÍNKOVÁ, Pavla LINHARTOVÁ and Ondřej PEŠ. Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry. In 24. Škola hmotnostní spektrometrie 2023. 2023. ISBN 978-80-88195-44-3.

Basic information

• Original name: Segmented Hair Cortisol Analysis By Online Solid Phase Extraction Liquid Chromatography Mass Spectrometry
• Authors: ŠMAK, Pavel (203 Czech Republic, belonging to the institution), Daniela FAČKOVCOVÁ (703 Slovakia, belonging to the institution), Katarína KOSTOLANSKÁ (703 Slovakia, belonging to the institution), Jana GREGOROVÁ (203 Czech Republic, belonging to the institution), Eliška BARTEČKOVÁ (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution), Libor USTOHAL (203 Czech Republic, belonging to the institution), Jana HOŘÍNKOVÁ (203 Czech Republic, belonging to the institution), Pavla LINHARTOVÁ (203 Czech Republic) and Ondřej PEŠ (203 Czech Republic, belonging to the institution).
• Edition: 24. Škola hmotnostní spektrometrie 2023, 2023.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 30202 Endocrinology and metabolism
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14110/23:00131876
• Organization unit: Faculty of Medicine
• ISBN: 978-80-88195-44-3
• Keywords in English: hair cortisol; liquid chromatography; mass spectrometry; probabilistic; Bayesian; longitudinal; evaluation

Abstract

The hypothalamic-pituitary-adrenal axis is the primary neurocrine axis that regulates response of the stress. Activation of this axis leads to releasing glucocorticoid (cortisol) that regulates physiological events and inhibits further HPA axis activation. Dysregulation of the hypothalamic-pituitary-adrenal axis plays an important role in a number of endocrine and psychiatric disorders, such as adrenal insufficiency, (ectopic) Cushing syndrome, major depressive disorder, borderline personality disorder, or schizophrenia. Hair cortisol concentration (HCC) belongs to relatively new methods of estimating cumulative cortisol exposure over months. While plasma, saliva, and urine cortisol levels provide immediate values in the organism, hair matrix allows for chronic exposure evaluation and longitudinal monitoring. Large inter- and intraindividual variability of the hair sampling and determination could be overcome by a segmentation approach – cutting the hair strand into smaller segments and analyzing them independently. Online (on-column) solid phase extraction liquid chromatography mass spectrometry (SPE LC MS) using a surrogate analyte has been performed to quantify HCC in various segmented hair samples. Based on the hair length, it was possible to obtain a relatively high number (3–6) of segments possibly containing highly correlated HCC values. Probabilistic multilevel (hierarchical) models were applied to volunteers’ hair for establishing a parameter of decrease, which could then be used to correct for such a decrease in the hair of the studied population. Consequently, the deviations from the corrections could then be attributed to the effect of fluctuation or variability of HCC in a certain time point (segment), e.g. episode, intervention, etc.

Links

• MUNI/A/1275/2022, interní kód MU: Name: Příspěvek chemických a biochemických technik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění

Investor: Masaryk University, The contribution of chemical and biochemical techniques to the study of the molecular nature of selected pathological conditions and diseases

• NU23-08-00257, research and development project: Name: Optimalizovaný longitudinální vlasový kortizol jako nástroj v diagnostice a monitorování psychiatrických poruch

Investor: Ministry of Health of the CR, Optimized longitudinal hair cortisol as a tool in diagnosis and monitoring of psychiatric disorders, Subprogram 1 - standard