Tau tubulin kinase 1 and 2 regulate ciliogenesis and human
pluripotent stem cells-derived neural rosettes

J 2023

Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes

BINÓ, Lucia and Lukáš ČAJÁNEK

Basic information

Original name

Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes

Authors

BINÓ, Lucia (703 Slovakia, guarantor, belonging to the institution) and Lukáš ČAJÁNEK (203 Czech Republic, belonging to the institution)

Edition

Scientific Reports, Berlin, Nature Research, 2023, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.600 in 2022

RIV identification code

RIV/00216224:14110/23:00131886

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1038/s41598-023-39887-9

UT WoS

001045574100047

Keywords in English

PRIMARY CILIA; CEP164; TTBK2; PHOSPHORYLATION; DIFFERENTIATION; PROLIFERATION; SPECIFICATION

Abstract

V originále

Primary cilia are key regulators of embryo development and tissue homeostasis. However, their mechanisms and functions, particularly in the context of human cells, are still unclear. Here, we analyzed the consequences of primary cilia modulation for human pluripotent stem cells (hPSCs) proliferation and differentiation. We report that neither activation of the cilia-associated Hedgehog signaling pathway nor ablation of primary cilia by CRISPR gene editing to knockout Tau Tubulin Kinase 2 (TTBK2), a crucial ciliogenesis regulator, affects the self-renewal of hPSCs. Further, we show that TTBK1, a related kinase without previous links to ciliogenesis, is upregulated during hPSCs-derived neural rosette differentiation. Importantly, we demonstrate that while TTBK1 fails to localize to the mother centriole, it regulates primary cilia formation in the differentiated, but not the undifferentiated hPSCs. Finally, we show that TTBK1/2 and primary cilia are implicated in the regulation of the size of hPSCs-derived neural rosettes.

Links

GA22-13277S, research and development project

Name: Ciliární Tau tubulin kináza 2 ve zdraví a nemoci: struktura a funkce

Investor: Czech Science Foundation

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/11/SUP/03/2022, interní kód MU

Name: Modelace ztráty ciliární funkce pomocí lidských pluripotentních buněk

Investor: Masaryk University, Start-up

90250, large research infrastructures

Name: Czech-BioImaging III

Citovat

BINÓ, Lucia and Lukáš ČAJÁNEK. Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes. Scientific Reports. Berlin: Nature Research, 2023, vol. 13, No 1, p. 1-13. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-023-39887-9.

@article{2324820,
   author = {Binó, Lucia and Čajánek, Lukáš},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-023-39887-9},
   keywords = {PRIMARY CILIA; CEP164; TTBK2; PHOSPHORYLATION; DIFFERENTIATION; PROLIFERATION; SPECIFICATION},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes},
   url = {https://www.nature.com/articles/s41598-023-39887-9},
   volume = {13},
   year = {2023}
}

TY  - JOUR
ID  - 2324820
AU  - Binó, Lucia - Čajánek, Lukáš
PY  - 2023
TI  - Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes
JF  - Scientific Reports
VL  - 13
IS  - 1
SP  - 1-13
EP  - 1-13
PB  - Nature Research
SN  - 20452322
KW  - PRIMARY CILIA
KW  - CEP164
KW  - TTBK2
KW  - PHOSPHORYLATION
KW  - DIFFERENTIATION
KW  - PROLIFERATION
KW  - SPECIFICATION
UR  - https://www.nature.com/articles/s41598-023-39887-9
N2  - Primary cilia are key regulators of embryo development and tissue homeostasis. However, their mechanisms and functions, particularly in the context of human cells, are still unclear. Here, we analyzed the consequences of primary cilia modulation for human pluripotent stem cells (hPSCs) proliferation and differentiation. We report that neither activation of the cilia-associated Hedgehog signaling pathway nor ablation of primary cilia by CRISPR gene editing to knockout Tau Tubulin Kinase 2 (TTBK2), a crucial ciliogenesis regulator, affects the self-renewal of hPSCs. Further, we show that TTBK1, a related kinase without previous links to ciliogenesis, is upregulated during hPSCs-derived neural rosette differentiation. Importantly, we demonstrate that while TTBK1 fails to localize to the mother centriole, it regulates primary cilia formation in the differentiated, but not the undifferentiated hPSCs. Finally, we show that TTBK1/2 and primary cilia are implicated in the regulation of the size of hPSCs-derived neural rosettes.
ER  -

BINÓ, Lucia and Lukáš ČAJÁNEK. Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes. \textit{Scientific Reports}. Berlin: Nature Research, 2023, vol.~13, No~1, p.~1-13. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-023-39887-9.

Detailed Information on Publication Record