Viral transcription-translation coupling in mammalian cells

a 2023

Viral transcription-translation coupling in mammalian cells

DAS, Amiyaranjan, Julia BARTULI, Clemens GRIMM, Utz FISCHER, Gabriel DEMO et. al.

Basic information

Original name

Viral transcription-translation coupling in mammalian cells

Authors

DAS, Amiyaranjan (356 India, belonging to the institution), Julia BARTULI (276 Germany), Clemens GRIMM (276 Germany), Utz FISCHER (276 Germany) and Gabriel DEMO (703 Slovakia, guarantor, belonging to the institution)

Edition

XIX Discussions in Structural Molecular Biology, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14740/23:00132138

Organization unit

Central European Institute of Technology

Keywords in English

translation ribosome cryoEM virus

Abstract

V originále

The coupling of transcription and translation (CTT) controls the gene regulation in bacteria. Recent cryo-electron microscopy (cryo-EM) studies showed the physical coupling of these two processes. Interestingly, the double-strand DNA Vaccinia virus (VACV) performs the viral genome replication, transcription, translation, and assembly of virions in infected mammalian cells within discrete cytoplasmic foci called viral factories. The initial rounds of viral transcription and translation during the early phase of infection occur inside the host cytoplasm. In intermediate and late-phase of infection the viral gene expression is carried out inside the viral factories in close association with host ribosomes. Here, we seek to uncover the potential regulation mechanism of viral gene expression via viral-host CTT. The primary approach is to reconstitute the CTT in vitro and use single particle cryo-EM to uncover the detailed view of the structural architecture of the viral-host CTT. We aim to employ cryo-electron tomography and correlated light and electron microscopy (CLEM) to directly visualize the viral factories in a near-native state at a sub-nanometer resolution to confirm the existence of viral-host CTT directly in VACV-infected cells.

Links

LL2008, research and development project

Name: Komunikace mezi transkripcí a translací (Acronym: CROSSBETT)

Investor: Ministry of Education, Youth and Sports of the CR, Crosstalk between transcription and translation

Citovat

DAS, Amiyaranjan, Julia BARTULI, Clemens GRIMM, Utz FISCHER and Gabriel DEMO. Viral transcription-translation coupling in mammalian cells. In XIX Discussions in Structural Molecular Biology. 2023.

@proceedings{2334202,
   author = {Das, Amiyaranjan and Bartuli, Julia and Grimm, Clemens and Fischer, Utz and Demo, Gabriel},
   booktitle = {XIX Discussions in Structural Molecular Biology},
   keywords = {translation ribosome cryoEM virus},
   language = {eng},
   title = {Viral transcription-translation coupling in mammalian cells},
   url = {https://cssb.structbio.org/xix-discussions-in-structural-molecular-biology-and-6th-user-meeting-of-ciisb-czech-infrastructure-for-integrative-structural-biology/},
   year = {2023}
}

TY  - CONF
ID  - 2334202
AU  - Das, Amiyaranjan - Bartuli, Julia - Grimm, Clemens - Fischer, Utz - Demo, Gabriel
PY  - 2023
TI  - Viral transcription-translation coupling in mammalian cells
KW  - translation ribosome cryoEM virus
UR  - https://cssb.structbio.org/xix-discussions-in-structural-molecular-biology-and-6th-user-meeting-of-ciisb-czech-infrastructure-for-integrative-structural-biology/
N2  - The coupling of transcription and translation (CTT) controls the gene regulation in bacteria. Recent cryo-electron microscopy (cryo-EM) studies showed the physical coupling of these two processes. Interestingly, the double-strand DNA Vaccinia virus (VACV) performs the viral genome replication, transcription, translation, and assembly of virions in infected mammalian cells within discrete cytoplasmic foci called viral factories. The initial rounds of viral transcription and translation during the early phase of infection occur inside the host cytoplasm. In intermediate and late-phase of infection the viral gene expression is carried out inside the viral factories in close association with host ribosomes. Here, we seek to uncover the potential regulation mechanism of viral gene expression via viral-host CTT. The primary approach is to reconstitute the CTT in vitro and use single particle cryo-EM to uncover the detailed view of the structural architecture of the viral-host CTT. We aim to employ cryo-electron tomography and correlated light and electron microscopy (CLEM) to directly visualize the viral factories in a near-native state at a sub-nanometer resolution to confirm the existence of viral-host CTT directly in VACV-infected cells.
ER  -

DAS, Amiyaranjan, Julia BARTULI, Clemens GRIMM, Utz FISCHER and Gabriel DEMO. Viral transcription-translation coupling in mammalian cells. In \textit{XIX Discussions in Structural Molecular Biology}. 2023.

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