Viral transcription-translation coupling in mammalian cells

DAS, Amiyaranjan, Julia BARTULI, Clemens GRIMM, Utz FISCHER and Gabriel DEMO. Viral transcription-translation coupling in mammalian cells. In XIX Discussions in Structural Molecular Biology. 2023.

Basic information

• Original name: Viral transcription-translation coupling in mammalian cells
• Authors: DAS, Amiyaranjan (356 India, belonging to the institution), Julia BARTULI (276 Germany), Clemens GRIMM (276 Germany), Utz FISCHER (276 Germany) and Gabriel DEMO (703 Slovakia, guarantor, belonging to the institution).
• Edition: XIX Discussions in Structural Molecular Biology, 2023.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/23:00132138
• Organization unit: Central European Institute of Technology
• Keywords in English: translation ribosome cryoEM virus
• Tags: rivok

Abstract

The coupling of transcription and translation (CTT) controls the gene regulation in bacteria. Recent cryo-electron microscopy (cryo-EM) studies showed the physical coupling of these two processes. Interestingly, the double-strand DNA Vaccinia virus (VACV) performs the viral genome replication, transcription, translation, and assembly of virions in infected mammalian cells within discrete cytoplasmic foci called viral factories. The initial rounds of viral transcription and translation during the early phase of infection occur inside the host cytoplasm. In intermediate and late-phase of infection the viral gene expression is carried out inside the viral factories in close association with host ribosomes. Here, we seek to uncover the potential regulation mechanism of viral gene expression via viral-host CTT. The primary approach is to reconstitute the CTT in vitro and use single particle cryo-EM to uncover the detailed view of the structural architecture of the viral-host CTT. We aim to employ cryo-electron tomography and correlated light and electron microscopy (CLEM) to directly visualize the viral factories in a near-native state at a sub-nanometer resolution to confirm the existence of viral-host CTT directly in VACV-infected cells.

Links

• LL2008, research and development project: Name: Komunikace mezi transkripcí a translací (Acronym: CROSSBETT)

Investor: Ministry of Education, Youth and Sports of the CR, Crosstalk between transcription and translation