J 2023

Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people

LESICZKA, Paulina Maria, Kristyna HRAZDILOVA, Vaclav HÖNIG, David MODRÝ, Ludek ZUREK et. al.

Basic information

Original name

Distant genetic variants of Anaplasma phagocytophilum from Ixodes ricinus attached to people

Authors

LESICZKA, Paulina Maria, Kristyna HRAZDILOVA, Vaclav HÖNIG, David MODRÝ (203 Czech Republic, belonging to the institution) and Ludek ZUREK (guarantor)

Edition

Parasites and Vectors, BioMed Central Ltd, 2023, 1756-3305

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30310 Parasitology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.200 in 2022

RIV identification code

RIV/00216224:14310/23:00132145

Organization unit

Faculty of Science

DOI

UT WoS

000941325000003

Keywords in English

Anaplasma phagocytophilum; Ixodes ricinus; Anaplasmosis; Genetic diversity; Infectious diseases

Tags

Tags

International impact, Reviewed
Změněno: 7/12/2023 09:29, Mgr. Marie Šípková, DiS.

Abstract

V originále

Background Although the tick-borne pathogen Anaplasma phagocytophilum is currently described as a single species, studies using genetic markers can distinguish groups of variants associated with different hosts, pathogenicity, zoonotic potential and biotic and geographic niches. The objective of our study was to investigate the genetic diversity of A. phagocytophilum and Ixodes ricinus ticks attached to people.Methods In collaboration with a commercial diagnostic company, a total of 52 DNA samples were obtained from ticks that tested positive for A. phagocytophilum by quantitative PCR. The genetic profile of each sample was determined using the groEL and ankA genes. Identification of the tick species was confirmed by partial sequencing of the COI subunit and a portion of the TROSPA gene.Results All 52 ticks were identified as I. ricinus. Two protocols of nested PCR amplifying 1293- and 407-bp fragments of groEL of A. phagocytophilum yielded amplicons of the expected size for all 52 samples. Among all sequences, we identified 10 unique genetic variants of groEL belonging to ecotype I and ecotype II. The analysis targeting ankA was successful in 46 of 52 ticks. Among all sequences, we identified 21 unique genetic variants phylogenetically belonging to three clusters.Conclusions Our results indicate that ticks attached to people harbor distant genetic variants of A. phagocytophilum, some of which are not recognized as zoonotic. Further studies are needed to determine the risk of human infection by genetic variants other than those designated as zoonotic.