BALÁŽ, Vojtech, Phoebe RIVORY, Douglas HAYWARD, Susan JAENSCH, Richard MALIK, Rogan LEE, David MODRÝ and Jan ŠLAPETA. Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid. PLOS Neglected Tropical Diseases. San Francisco: Public Library of Science, 2023, vol. 17, No 5, p. 1-14. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0011038.
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Basic information
Original name Angie-LAMP for diagnosis of human eosinophilic meningitis using dog as proxy: A LAMP assay for Angiostrongylus cantonensis DNA in cerebrospinal fluid
Authors BALÁŽ, Vojtech, Phoebe RIVORY, Douglas HAYWARD, Susan JAENSCH, Richard MALIK, Rogan LEE, David MODRÝ (203 Czech Republic, guarantor, belonging to the institution) and Jan ŠLAPETA.
Edition PLOS Neglected Tropical Diseases, San Francisco, Public Library of Science, 2023, 1935-2735.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30310 Parasitology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.800 in 2022
RIV identification code RIV/00216224:14310/23:00132146
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1371/journal.pntd.0011038
UT WoS 000984025300006
Keywords in English Angiostrongyliasis; Angiostrongylus; Angiostrongylus cantonensis; Animals; DNA; Ribosomal; Dogs; Humans; LAMP assay; Meningitis; Rats; Snails; Strongylida Infections
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 7/12/2023 08:34.
Abstract
Background Angiostrongylus cantonensis (rat lungworm) is recognised as the leading cause of human eosinophilic meningitis, a serious condition observed when nematode larvae migrate through the CNS. Canine Neural Angiostrongyliasis (CNA) is the analogous disease in dogs. Both humans and dogs are accidental hosts, and a rapid diagnosis is warranted. A highly sensitive PCR based assay is available but often not readily accessible in many jurisdictions. An alternative DNA amplification assay that would further improve accessibility is needed. This study aimed to assess the diagnostic utility of a newly designed LAMP assay to detect DNA of globally distributed and invasive A. cantonensis and Angiostrongylus mackerrasae, the other neurotropic Angiostrongylus species, which is native to Australia. Methodology/Principal findings Cerebrospinal fluid (CSF) from dogs with a presumptive diagnosis of A. cantonensis infection (2020–2022) were received for confirmatory laboratory testing and processed for DNA isolation and ultrasensitive Angiostrongylus qPCR targeting AcanR3390. A newly designed LAMP assay targeting the same gene target was directly compared to the reference ultrasensitive qPCR in a diagnostic laboratory setting to determine the presence of A. cantonensis DNA to diagnose CNA. The LAMP assay (Angie-LAMP) allowed the sensitive detection of A. cantonensis DNA from archived DNA specimens (Kappa = 0.81, 95%CI 0.69–0.92; n = 93) and rapid single-step lysis of archived CSF samples (Kappa = 0.77, 95%CI 0.59–0.94; n = 52). Only A. cantonensis DNA was detected in canine CSF samples, and co-infection with A. mackerrasae using amplicon deep sequencing (ITS-2 rDNA) was not demonstrated. Both SYD.1 and AC13 haplotypes were detected using sequencing of partial cox1. Conclusions/Significance The Angie-LAMP assay is a useful molecular tool for detecting Angiostrongylus DNA in canine CSF and performs comparably to a laboratory Angiostrongylus qPCR. Adaptation of single-step sample lysis improved potential applicability for diagnosis of angiostrongyliasis in a clinical setting for dogs and by extension, to humans.
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