Detailed Information on Publication Record
2024
Changes in body size and fertility due to artificial and natural feeding of laboratory common bed bugs (Heteroptera: Cimicidae)
SASÍNKOVÁ, Markéta, Jana KŘEMENOVÁ, Petr CHAJMA, Tomáš BARTONIČKA, Christian MASSINO et. al.Basic information
Original name
Changes in body size and fertility due to artificial and natural feeding of laboratory common bed bugs (Heteroptera: Cimicidae)
Authors
SASÍNKOVÁ, Markéta, Jana KŘEMENOVÁ (203 Czech Republic, belonging to the institution), Petr CHAJMA (203 Czech Republic), Tomáš BARTONIČKA (203 Czech Republic, belonging to the institution), Christian MASSINO (276 Germany), Oliver OTTI (276 Germany) and Ondrej BALVIN (203 Czech Republic, guarantor)
Edition
Journal of Medical Entomology, Cary, NC, Oxford Univ Press Inc, 2024, 0022-2585
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10613 Zoology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.100 in 2022
Organization unit
Faculty of Science
UT WoS
001088393400001
Keywords in English
insect rearing; diet; blood-sucking insect; number of eggs; blood preservation
Tags
Tags
International impact, Reviewed
Změněno: 27/2/2024 08:46, Mgr. Marie Šípková, DiS.
Abstract
V originále
Rearing common bed bugs (Cimex lectularius L.) and other hematophagous insects is essential for basic, medical, and pest-control research. Logistically, acquiring fresh blood can be a challenge, while biologically, the eventual effects of different rearing and blood preparation protocols on bed bug genotype and phenotype pose a risk of biased research results. Using bed bug populations that are either bat- (BL) or human-related (HL), we tested the short- and long-term effects of rearing bugs on live bats or human volunteers, or artificially on CPDA (citrate phosphate dextrose, adenine)-treated blood, measuring meal size, body size, and fertility. We found that artificial feeding did not affect meal size compared with feeding on natural hosts. Long-term rearing across many generations of HL on CPDA-preserved blood led to reduced body size and fertility compared with populations reared on human volunteers. Blood preservatives increased the proportion of sterile eggs even after a single feed. Finally, our results indicated that laboratory reared bed bugs were smaller, regardless of the blood source, than wild bugs. Similar effects of artificial feeding or laboratory rearing alone should be considered in future studies using bed bug cultures to choose an appropriate rearing protocol. With regard to switching between bat and human hosts, HL took smaller meals and BL had lower fertility when fed on bats than when fed on humans. We attribute these results to methodological constrains, specifically the inconsistency of bat feeding, rather than to host specialization. Nevertheless, BL can be easily reared using human blood and artificial feeding systems.
Links
| GC18-08468J, research and development project |
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