J 2023

Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis

KREJCI, Jana, Orazio Angelo ARCIDIACONO, Radim ČEGAN, Katarzyna Anna RADASZKIEWICZ, Jiří PACHERNÍK et. al.

Basic information

Original name

Cell Differentiation and Aging Lead To Up-Regulation of FTO, While the ALKBH5 Protein Level Was Stable During Aging but Up-Regulated During in vitro-Induced Cardiomyogenesis

Authors

KREJCI, Jana (203 Czech Republic), Orazio Angelo ARCIDIACONO (380 Italy), Radim ČEGAN (203 Czech Republic), Katarzyna Anna RADASZKIEWICZ (616 Poland, belonging to the institution), Jiří PACHERNÍK (203 Czech Republic, belonging to the institution), Jan PIRK (203 Czech Republic), Martin PEŠL (203 Czech Republic, belonging to the institution), Petr FILA (203 Czech Republic, belonging to the institution) and Eva BARTOVA

Edition

Physiological research, Praha, Fyziologický ústav AV ČR, 2023, 0862-8408

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30201 Cardiac and Cardiovascular systems

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.100 in 2022

RIV identification code

RIV/00216224:14310/23:00132199

Organization unit

Faculty of Science

DOI

UT WoS

001078316100002

Keywords in English

mES cells; hES cells; FTO; ALKBH5; Epigenetics; Differentiation; Aging

Tags

Tags

International impact, Reviewed
Změněno: 11/3/2024 10:13, Mgr. Marie Šípková, DiS.

Abstract

V originále

FTO and ALKBH5 proteins are essential erasers of N6-adenosine methylation in RNA. We studied how levels of FTO and ALKBH5 proteins changed during mouse embryonic development, aging, cardiomyogenesis, and neuroectodermal differentiation. We observed that aging in male and female mice was associated with FTO up-regulation in mouse hearts, brains, lungs, and kidneys, while the ALKBH5 level remained stable. FTO and ALKBH5 proteins were up-regulated during experimentally induced cardiomyogenesis, but the level of ALKBH5 protein was not changed when neuroectodermal differentiation was induced. HDAC1 depletion in mouse ES cells caused FTO down-regulation. In these cells, mRNA, carrying information from genes that regulate histone signature, RNA processing, and cell differentiation, was characterized by a reduced level of N6-adenosine methylation in specific gene loci, primarily regulating cell differentiation into neuroectoderm. Together, when we compared both RNA demethylating proteins, the FTO protein level undergoes the most significant changes during cell differentiation and aging. Thus, we conclude that during aging and neuronal differentiation, m6A RNA demethylation is likely regulated by the FTO protein but not via the function of ALKBH5.