A new device for online nanoscale sampling and capillary
electrophoresis analysis of plant sap composition

J 2024

A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition

MELICHEROVÁ, Natália, Vít GLOSER, Jan SKALÁK, Martin TRTILEK, Jana LAVICKÁ et. al.

Basic information

Original name

A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition

Authors

MELICHEROVÁ, Natália (703 Slovakia, belonging to the institution), Vít GLOSER (203 Czech Republic, belonging to the institution), Jan SKALÁK (203 Czech Republic, belonging to the institution), Martin TRTILEK, Jana LAVICKÁ (203 Czech Republic, guarantor) and František FORET (203 Czech Republic)

Edition

Electrophoresis, Hoboken, Wiley-Blackwell, 2024, 0173-0835

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10609 Biochemical research methods

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.900 in 2022

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/elps.202300201

UT WoS

001090485100001

Keywords in English

capillary electrophoresis; plant sap; sampling; stress; xylem

Abstract

V originále

In this work, an online sampling of plant xylem sap combined with an efficient (CE)-based method was developed and applied to study the kinetics of changes in the sap composition and to assess plant fitness under stress conditions comprehensively. A laboratory-built CE device was developed to provide online sampling and CE analysis of various ionogenic species in the sap during plant stress response. The rapid online sampling and short CE analysis time allow for real-time monitoring of changes in sap constituents in the living plant during the stress response. The developed device was successfully used to analyze chloride, nitrate, and sulfate ions in the plant xylem during the salt stress or stress caused by nitrate deficiency within short time scales.

Links

EF16_026/0008446, research and development project

Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

Citovat

MELICHEROVÁ, Natália, Vít GLOSER, Jan SKALÁK, Martin TRTILEK, Jana LAVICKÁ and František FORET. A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition. Electrophoresis. Hoboken: Wiley-Blackwell, 2024, vol. 45, 3-4, p. 310-317. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.202300201.

@article{2339080,
   author = {Melicherová, Natália and Gloser, Vít and Skalák, Jan and Trtilek, Martin and Lavická, Jana and Foret, František},
   article_location = {Hoboken},
   article_number = {3-4},
   doi = {http://dx.doi.org/10.1002/elps.202300201},
   keywords = {capillary electrophoresis; plant sap; sampling; stress; xylem},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202300201},
   volume = {45},
   year = {2024}
}

TY  - JOUR
ID  - 2339080
AU  - Melicherová, Natália - Gloser, Vít - Skalák, Jan - Trtilek, Martin - Lavická, Jana - Foret, František
PY  - 2024
TI  - A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition
JF  - Electrophoresis
VL  - 45
IS  - 3-4
SP  - 310-317
EP  - 310-317
PB  - Wiley-Blackwell
SN  - 01730835
KW  - capillary electrophoresis
KW  - plant sap
KW  - sampling
KW  - stress
KW  - xylem
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elps.202300201
N2  - In this work, an online sampling of plant xylem sap combined with an efficient (CE)-based method was developed and applied to study the kinetics of changes in the sap composition and to assess plant fitness under stress conditions comprehensively. A laboratory-built CE device was developed to provide online sampling and CE analysis of various ionogenic species in the sap during plant stress response. The rapid online sampling and short CE analysis time allow for real-time monitoring of changes in sap constituents in the living plant during the stress response. The developed device was successfully used to analyze chloride, nitrate, and sulfate ions in the plant xylem during the salt stress or stress caused by nitrate deficiency within short time scales.
ER  -

MELICHEROVÁ, Natália, Vít GLOSER, Jan SKALÁK, Martin TRTILEK, Jana LAVICKÁ and František FORET. A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition. \textit{Electrophoresis}. Hoboken: Wiley-Blackwell, 2024, vol.~45, 3-4, p.~310-317. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.202300201.

Detailed Information on Publication Record