MELICHEROVÁ, Natália, Vít GLOSER, Jan SKALÁK, Martin TRTILEK, Jana LAVICKÁ and František FORET. A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition. Electrophoresis. Hoboken: Wiley-Blackwell, 2024, vol. 45, 3-4, p. 310-317. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.202300201.
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Basic information
Original name A new device for online nanoscale sampling and capillary electrophoresis analysis of plant sap composition
Authors MELICHEROVÁ, Natália (703 Slovakia, belonging to the institution), Vít GLOSER (203 Czech Republic, belonging to the institution), Jan SKALÁK (203 Czech Republic, belonging to the institution), Martin TRTILEK, Jana LAVICKÁ (203 Czech Republic, guarantor) and František FORET (203 Czech Republic).
Edition Electrophoresis, Hoboken, Wiley-Blackwell, 2024, 0173-0835.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10609 Biochemical research methods
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 2.900 in 2022
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1002/elps.202300201
UT WoS 001090485100001
Keywords in English capillary electrophoresis; plant sap; sampling; stress; xylem
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Eva Dubská, učo 77638. Changed: 27/3/2024 09:19.
Abstract
In this work, an online sampling of plant xylem sap combined with an efficient (CE)-based method was developed and applied to study the kinetics of changes in the sap composition and to assess plant fitness under stress conditions comprehensively. A laboratory-built CE device was developed to provide online sampling and CE analysis of various ionogenic species in the sap during plant stress response. The rapid online sampling and short CE analysis time allow for real-time monitoring of changes in sap constituents in the living plant during the stress response. The developed device was successfully used to analyze chloride, nitrate, and sulfate ions in the plant xylem during the salt stress or stress caused by nitrate deficiency within short time scales.
Links
EF16_026/0008446, research and development projectName: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin
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