| GUERRA-SLOMPO, Eloise Pavao, Gisele Fernanda Assine PICCHI-CONSTANTE, Martin MAREK, Christophe ROMIER, Wolfgang SIPPL and Nilson Ivo Tonin ZANCHIN. In cellulo and in vivo assays for compound testing against Trypanosoma cruzi. STAR Protocols. United States: Cell Press, 2023, vol. 4, No 1, p. 1-42. ISSN 2666-1667. Available from: https://dx.doi.org/10.1016/j.xpro.2023.102058. |
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@article{2339437,
author = {GuerraandSlompo, Eloise Pavao and PicchiandConstante, Gisele Fernanda Assine and Marek, Martin and Romier, Christophe and Sippl, Wolfgang and Zanchin, Nilson Ivo Tonin},
article_location = {United States},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.xpro.2023.102058},
keywords = {Cell Biology; Cell-based Assays; High-throughput Screening; Microbiology; Model Organisms},
language = {eng},
issn = {2666-1667},
journal = {STAR Protocols},
title = {In cellulo and in vivo assays for compound testing against Trypanosoma cruzi},
url = {https://www.sciencedirect.com/science/article/pii/S2666166723000163?via%3Dihub},
volume = {4},
year = {2023}
}
TY - JOUR
ID - 2339437
AU - Guerra-Slompo, Eloise Pavao - Picchi-Constante, Gisele Fernanda Assine - Marek, Martin - Romier, Christophe - Sippl, Wolfgang - Zanchin, Nilson Ivo Tonin
PY - 2023
TI - In cellulo and in vivo assays for compound testing against Trypanosoma cruzi
JF - STAR Protocols
VL - 4
IS - 1
SP - 1-42
EP - 1-42
PB - Cell Press
SN - 26661667
KW - Cell Biology
KW - Cell-based Assays
KW - High-throughput Screening
KW - Microbiology
KW - Model Organisms
UR - https://www.sciencedirect.com/science/article/pii/S2666166723000163?via%3Dihub
N2 - Here, we describe a combined in cellulo and in vivo approach to identify com-pounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determina-tion. Compounds showing high SI are tested using in vivo infection models in par-allel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment. For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).1
ER -
GUERRA-SLOMPO, Eloise Pavao, Gisele Fernanda Assine PICCHI-CONSTANTE, Martin MAREK, Christophe ROMIER, Wolfgang SIPPL and Nilson Ivo Tonin ZANCHIN. In cellulo and in vivo assays for compound testing against Trypanosoma cruzi. \textit{STAR Protocols}. United States: Cell Press, 2023, vol.~4, No~1, p.~1-42. ISSN~2666-1667. Available from: https://dx.doi.org/10.1016/j.xpro.2023.102058.
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