In cellulo and in vivo assays for compound testing against
Trypanosoma cruzi

J 2023

In cellulo and in vivo assays for compound testing against Trypanosoma cruzi

GUERRA-SLOMPO, Eloise Pavao, Gisele Fernanda Assine PICCHI-CONSTANTE, Martin MAREK, Christophe ROMIER, Wolfgang SIPPL et. al.

Basic information

Original name

In cellulo and in vivo assays for compound testing against Trypanosoma cruzi

Authors

GUERRA-SLOMPO, Eloise Pavao, Gisele Fernanda Assine PICCHI-CONSTANTE, Martin MAREK (203 Czech Republic, guarantor, belonging to the institution), Christophe ROMIER, Wolfgang SIPPL and Nilson Ivo Tonin ZANCHIN

Edition

STAR Protocols, United States, Cell Press, 2023, 2666-1667

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/23:00132246

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.xpro.2023.102058

UT WoS

001058390100001

Keywords in English

Cell Biology; Cell-based Assays; High-throughput Screening; Microbiology; Model Organisms

Abstract

V originále

Here, we describe a combined in cellulo and in vivo approach to identify com-pounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determina-tion. Compounds showing high SI are tested using in vivo infection models in par-allel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment. For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).1

Citovat

GUERRA-SLOMPO, Eloise Pavao, Gisele Fernanda Assine PICCHI-CONSTANTE, Martin MAREK, Christophe ROMIER, Wolfgang SIPPL and Nilson Ivo Tonin ZANCHIN. In cellulo and in vivo assays for compound testing against Trypanosoma cruzi. STAR Protocols. United States: Cell Press, 2023, vol. 4, No 1, p. 1-42. ISSN 2666-1667. Available from: https://dx.doi.org/10.1016/j.xpro.2023.102058.

@article{2339437,
   author = {GuerraandSlompo, Eloise Pavao and PicchiandConstante, Gisele Fernanda Assine and Marek, Martin and Romier, Christophe and Sippl, Wolfgang and Zanchin, Nilson Ivo Tonin},
   article_location = {United States},
   article_number = {1},
   doi = {http://dx.doi.org/10.1016/j.xpro.2023.102058},
   keywords = {Cell Biology; Cell-based Assays; High-throughput Screening; Microbiology; Model Organisms},
   language = {eng},
   issn = {2666-1667},
   journal = {STAR Protocols},
   title = {In cellulo and in vivo assays for compound testing against Trypanosoma cruzi},
   url = {https://www.sciencedirect.com/science/article/pii/S2666166723000163?via%3Dihub},
   volume = {4},
   year = {2023}
}

TY  - JOUR
ID  - 2339437
AU  - Guerra-Slompo, Eloise Pavao - Picchi-Constante, Gisele Fernanda Assine - Marek, Martin - Romier, Christophe - Sippl, Wolfgang - Zanchin, Nilson Ivo Tonin
PY  - 2023
TI  - In cellulo and in vivo assays for compound testing against Trypanosoma cruzi
JF  - STAR Protocols
VL  - 4
IS  - 1
SP  - 1-42
EP  - 1-42
PB  - Cell Press
SN  - 26661667
KW  - Cell Biology
KW  - Cell-based Assays
KW  - High-throughput Screening
KW  - Microbiology
KW  - Model Organisms
UR  - https://www.sciencedirect.com/science/article/pii/S2666166723000163?via%3Dihub
N2  - Here, we describe a combined in cellulo and in vivo approach to identify com-pounds with higher potential for efficient inhibition of Trypanosoma cruzi. Phase I of in cellulo assays is designed to exclude inactive or toxic compounds, while phase II is designed for accurate IC50, CC50, and selective index (SI) determina-tion. Compounds showing high SI are tested using in vivo infection models in par-allel with benznidazole to assess their efficacy relative to a reference drug used for Chagas disease treatment. For complete details on the use and execution of this protocol, please refer to Marek et al. (2021).1
ER  -

GUERRA-SLOMPO, Eloise Pavao, Gisele Fernanda Assine PICCHI-CONSTANTE, Martin MAREK, Christophe ROMIER, Wolfgang SIPPL and Nilson Ivo Tonin ZANCHIN. In cellulo and in vivo assays for compound testing against Trypanosoma cruzi. \textit{STAR Protocols}. United States: Cell Press, 2023, vol.~4, No~1, p.~1-42. ISSN~2666-1667. Available from: https://dx.doi.org/10.1016/j.xpro.2023.102058.

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