YAP Signaling Regulates the Cellular Uptake and Therapeutic
Effect of Nanoparticles

J 2024

YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles

CASSANI, Marco, Soraia FERNANDES, Jorge Oliver-De La CRUZ, Helena DURIKOVA, Jan VRBSKY et. al.

Basic information

Original name

YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles

Authors

CASSANI, Marco, Soraia FERNANDES, Jorge Oliver-De La CRUZ, Helena DURIKOVA, Jan VRBSKY, Marek PATOČKA, Veronika HEGROVA, Šimon KLIMOVIČ (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Doriana DEBELLIS, Petr SKLÁDAL (203 Czech Republic, belonging to the institution), Francesca CAVALIERI, Frank CARUSO and Giancarlo FORTE (guarantor)

Edition

Advanced Science, Wiley-VCH GmbH, 2024, 2198-3844

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

21000 2.10 Nano-technology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 15.100 in 2022

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/advs.202302965

UT WoS

001121056800001

Keywords in English

cell-nanoparticle interactions; nanotherapies; yes-associated protein; breast cancer

Abstract

V originále

Interactions between living cells and nanoparticles are extensively studied to enhance the delivery of therapeutics. Nanoparticles size, shape, stiffness, and surface charge are regarded as the main features able to control the fate of cell-nanoparticle interactions. However, the clinical translation of nanotherapies has so far been limited, and there is a need to better understand the biology of cell-nanoparticle interactions. This study investigates the role of cellular mechanosensitive components in cell-nanoparticle interactions. It is demonstrated that the genetic and pharmacologic inhibition of yes-associated protein (YAP), a key component of cancer cell mechanosensing apparatus and Hippo pathway effector, improves nanoparticle internalization in triple-negative breast cancer cells regardless of nanoparticle properties or substrate characteristics. This process occurs through YAP-dependent regulation of endocytic pathways, cell mechanics, and membrane organization. Hence, the study proposes targeting YAP may sensitize triple-negative breast cancer cells to chemotherapy and increase the selectivity of nanotherapy.

Links

EF18_046/0015974, research and development project

Name: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2023042, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministry of Education, Youth and Sports of the CR, CIISB - Czech Infrastructure for Integrative Structural Biology

90132, large research infrastructures

Name: NCMG II

Citovat

CASSANI, Marco, Soraia FERNANDES, Jorge Oliver-De La CRUZ, Helena DURIKOVA, Jan VRBSKY, Marek PATOČKA, Veronika HEGROVA, Šimon KLIMOVIČ, Jan PŘIBYL, Doriana DEBELLIS, Petr SKLÁDAL, Francesca CAVALIERI, Frank CARUSO and Giancarlo FORTE. YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles. Advanced Science. Wiley-VCH GmbH, 2024, vol. 11, No 2, p. 1-16. ISSN 2198-3844. Available from: https://dx.doi.org/10.1002/advs.202302965.

@article{2339561,
   author = {Cassani, Marco and Fernandes, Soraia and Cruz, Jorge OliverandDe La and Durikova, Helena and Vrbsky, Jan and Patočka, Marek and Hegrova, Veronika and Klimovič, Šimon and Přibyl, Jan and Debellis, Doriana and Skládal, Petr and Cavalieri, Francesca and Caruso, Frank and Forte, Giancarlo},
   article_number = {2},
   doi = {http://dx.doi.org/10.1002/advs.202302965},
   keywords = {cell-nanoparticle interactions; nanotherapies; yes-associated protein; breast cancer},
   language = {eng},
   issn = {2198-3844},
   journal = {Advanced Science},
   title = {YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles},
   url = {https://doi.org/10.1002/advs.202302965},
   volume = {11},
   year = {2024}
}

TY  - JOUR
ID  - 2339561
AU  - Cassani, Marco - Fernandes, Soraia - Cruz, Jorge Oliver-De La - Durikova, Helena - Vrbsky, Jan - Patočka, Marek - Hegrova, Veronika - Klimovič, Šimon - Přibyl, Jan - Debellis, Doriana - Skládal, Petr - Cavalieri, Francesca - Caruso, Frank - Forte, Giancarlo
PY  - 2024
TI  - YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles
JF  - Advanced Science
VL  - 11
IS  - 2
SP  - 1-16
EP  - 1-16
PB  - Wiley-VCH GmbH
SN  - 21983844
KW  - cell-nanoparticle interactions
KW  - nanotherapies
KW  - yes-associated protein
KW  - breast cancer
UR  - https://doi.org/10.1002/advs.202302965
N2  - Interactions between living cells and nanoparticles are extensively studied to enhance the delivery of therapeutics. Nanoparticles size, shape, stiffness, and surface charge are regarded as the main features able to control the fate of cell-nanoparticle interactions. However, the clinical translation of nanotherapies has so far been limited, and there is a need to better understand the biology of cell-nanoparticle interactions. This study investigates the role of cellular mechanosensitive components in cell-nanoparticle interactions. It is demonstrated that the genetic and pharmacologic inhibition of yes-associated protein (YAP), a key component of cancer cell mechanosensing apparatus and Hippo pathway effector, improves nanoparticle internalization in triple-negative breast cancer cells regardless of nanoparticle properties or substrate characteristics. This process occurs through YAP-dependent regulation of endocytic pathways, cell mechanics, and membrane organization. Hence, the study proposes targeting YAP may sensitize triple-negative breast cancer cells to chemotherapy and increase the selectivity of nanotherapy.
ER  -

CASSANI, Marco, Soraia FERNANDES, Jorge Oliver-De La CRUZ, Helena DURIKOVA, Jan VRBSKY, Marek PATOČKA, Veronika HEGROVA, Šimon KLIMOVIČ, Jan PŘIBYL, Doriana DEBELLIS, Petr SKLÁDAL, Francesca CAVALIERI, Frank CARUSO and Giancarlo FORTE. YAP Signaling Regulates the Cellular Uptake and Therapeutic Effect of Nanoparticles. \textit{Advanced Science}. Wiley-VCH GmbH, 2024, vol.~11, No~2, p.~1-16. ISSN~2198-3844. Available from: https://dx.doi.org/10.1002/advs.202302965.

Detailed Information on Publication Record