Dynamic release of neuronal extracellular vesicles containing
miR-21a-5p is induced by hypoxia

J 2023

Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia

KORVENLAITA, Nea, Mireia GOMEZ-BUDIA, Flavia SCOYNI, Cristiana PISTONO, Luca GIUDICE et. al.

Basic information

Original name

Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia

Authors

KORVENLAITA, Nea (guarantor), Mireia GOMEZ-BUDIA, Flavia SCOYNI, Cristiana PISTONO, Luca GIUDICE, Shaila EAMEN, Sanna LOPPI, de Sande Ana HERNANDEZ, Benjamin HUREMAGIC, Maria BOUVY-LIIVRAND, Merja HEINANIEMI, Minna U KAIKKONEN, Lesley CHENG, Andrew F HILL, Katja M KANNINEN, Guido W JENSTER, Martin E VAN ROYEN, Laura RAMIRO, Joan MONTANER, Tereza BÁŤKOVÁ (203 Czech Republic, belonging to the institution), Robert MIKULÍK (203 Czech Republic, belonging to the institution), Rosalba GIUGNO, Jukka JOLKKONEN, Paula KORHONEN and Tarja MALM

Edition

Journal of Extracellular Vesicles, HOBOKEN, WILEY, 2023, 2001-3078

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30210 Clinical neurology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 16.000 in 2022

RIV identification code

RIV/00216224:14110/23:00132277

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1002/jev2.12297

UT WoS

000906496700001

Keywords in English

biomarkers; extracellular vesicle; hypoxia; ischemic stroke; miR-21a-5p; neuron

Abstract

V originále

Hypoxia induces changes in the secretion of extracellular vesicles (EVs) in several non-neuronal cells and pathological conditions. EVs are packed with biomolecules, such as microRNA(miR)-21-5p, which respond to hypoxia. However, the true EV association of miR-21-5p, and its functional or biomarker relevance, are inadequately characterised. Neurons are extremely sensitive cells, and it is not known whether the secretion of neuronal EVs and miR-21-5p are altered upon hypoxia. Here, we characterised the temporal EV secretion profile and cell viability of neurons under hypoxia. Hypoxia induced a rapid increase of miR-21a-5p secretion in the EVs, which preceded the elevation of hypoxia-induced tissue or cellular miR-21a-5p. Prolonged hypoxia induced cell death and the release of morphologically distinct EVs. The EVs protected miR-21a-5p from enzymatic degradation but a remarkable fraction of miR-21a-5p remained fragile and non-EV associated. The increase in miR-21a-5p secretion may have biomarker potential, as high blood levels of miR-21-5p in stroke patients were associated with significant disability at hospital discharge. Our data provides an understanding of the dynamic regulation of EV secretion from neurons under hypoxia and provides a candidate for the prediction of recovery from ischemic stroke.

Citovat

KORVENLAITA, Nea, Mireia GOMEZ-BUDIA, Flavia SCOYNI, Cristiana PISTONO, Luca GIUDICE, Shaila EAMEN, Sanna LOPPI, de Sande Ana HERNANDEZ, Benjamin HUREMAGIC, Maria BOUVY-LIIVRAND, Merja HEINANIEMI, Minna U KAIKKONEN, Lesley CHENG, Andrew F HILL, Katja M KANNINEN, Guido W JENSTER, Martin E VAN ROYEN, Laura RAMIRO, Joan MONTANER, Tereza BÁŤKOVÁ, Robert MIKULÍK, Rosalba GIUGNO, Jukka JOLKKONEN, Paula KORHONEN and Tarja MALM. Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia. Journal of Extracellular Vesicles. HOBOKEN: WILEY, 2023, vol. 12, No 1, p. 1-21. ISSN 2001-3078. Available from: https://dx.doi.org/10.1002/jev2.12297.

@article{2339679,
   author = {Korvenlaita, Nea and GomezandBudia, Mireia and Scoyni, Flavia and Pistono, Cristiana and Giudice, Luca and Eamen, Shaila and Loppi, Sanna and Hernandez, de Sande Ana and Huremagic, Benjamin and BouvyandLiivrand, Maria and Heinaniemi, Merja and Kaikkonen, Minna U and Cheng, Lesley and Hill, Andrew F and Kanninen, Katja M and Jenster, Guido W and van Royen, Martin E and Ramiro, Laura and Montaner, Joan and Báťková, Tereza and Mikulík, Robert and Giugno, Rosalba and Jolkkonen, Jukka and Korhonen, Paula and Malm, Tarja},
   article_location = {HOBOKEN},
   article_number = {1},
   doi = {http://dx.doi.org/10.1002/jev2.12297},
   keywords = {biomarkers; extracellular vesicle; hypoxia; ischemic stroke; miR-21a-5p; neuron},
   language = {eng},
   issn = {2001-3078},
   journal = {Journal of Extracellular Vesicles},
   title = {Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/jev2.12297},
   volume = {12},
   year = {2023}
}

TY  - JOUR
ID  - 2339679
AU  - Korvenlaita, Nea - Gomez-Budia, Mireia - Scoyni, Flavia - Pistono, Cristiana - Giudice, Luca - Eamen, Shaila - Loppi, Sanna - Hernandez, de Sande Ana - Huremagic, Benjamin - Bouvy-Liivrand, Maria - Heinaniemi, Merja - Kaikkonen, Minna U - Cheng, Lesley - Hill, Andrew F - Kanninen, Katja M - Jenster, Guido W - van Royen, Martin E - Ramiro, Laura - Montaner, Joan - Báťková, Tereza - Mikulík, Robert - Giugno, Rosalba - Jolkkonen, Jukka - Korhonen, Paula - Malm, Tarja
PY  - 2023
TI  - Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia
JF  - Journal of Extracellular Vesicles
VL  - 12
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - WILEY
SN  - 20013078
KW  - biomarkers
KW  - extracellular vesicle
KW  - hypoxia
KW  - ischemic stroke
KW  - miR-21a-5p
KW  - neuron
UR  - https://onlinelibrary.wiley.com/doi/10.1002/jev2.12297
N2  - Hypoxia induces changes in the secretion of extracellular vesicles (EVs) in several non-neuronal cells and pathological conditions. EVs are packed with biomolecules, such as microRNA(miR)-21-5p, which respond to hypoxia. However, the true EV association of miR-21-5p, and its functional or biomarker relevance, are inadequately characterised. Neurons are extremely sensitive cells, and it is not known whether the secretion of neuronal EVs and miR-21-5p are altered upon hypoxia. Here, we characterised the temporal EV secretion profile and cell viability of neurons under hypoxia. Hypoxia induced a rapid increase of miR-21a-5p secretion in the EVs, which preceded the elevation of hypoxia-induced tissue or cellular miR-21a-5p. Prolonged hypoxia induced cell death and the release of morphologically distinct EVs. The EVs protected miR-21a-5p from enzymatic degradation but a remarkable fraction of miR-21a-5p remained fragile and non-EV associated. The increase in miR-21a-5p secretion may have biomarker potential, as high blood levels of miR-21-5p in stroke patients were associated with significant disability at hospital discharge. Our data provides an understanding of the dynamic regulation of EV secretion from neurons under hypoxia and provides a candidate for the prediction of recovery from ischemic stroke.
ER  -

KORVENLAITA, Nea, Mireia GOMEZ-BUDIA, Flavia SCOYNI, Cristiana PISTONO, Luca GIUDICE, Shaila EAMEN, Sanna LOPPI, de Sande Ana HERNANDEZ, Benjamin HUREMAGIC, Maria BOUVY-LIIVRAND, Merja HEINANIEMI, Minna U KAIKKONEN, Lesley CHENG, Andrew F HILL, Katja M KANNINEN, Guido W JENSTER, Martin E VAN ROYEN, Laura RAMIRO, Joan MONTANER, Tereza BÁŤKOVÁ, Robert MIKULÍK, Rosalba GIUGNO, Jukka JOLKKONEN, Paula KORHONEN and Tarja MALM. Dynamic release of neuronal extracellular vesicles containing miR-21a-5p is induced by hypoxia. \textit{Journal of Extracellular Vesicles}. HOBOKEN: WILEY, 2023, vol.~12, No~1, p.~1-21. ISSN~2001-3078. Available from: https://dx.doi.org/10.1002/jev2.12297.

Detailed Information on Publication Record