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@article{2339698,
author = {Bobalova, Janette and Strouhalova, Dana and Bobáľ, Pavel},
article_location = {WASHINGTON},
article_number = {41},
doi = {http://dx.doi.org/10.1021/acs.jafc.3c00886},
keywords = {barley; post-translationalmodification; protein; mass spectrometry},
language = {eng},
issn = {0021-8561},
journal = {Journal of Agricultural and Food Chemistry},
title = {Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley},
url = {https://pubs.acs.org/doi/10.1021/acs.jafc.3c00886},
volume = {71},
year = {2023}
}
TY - JOUR
ID - 2339698
AU - Bobalova, Janette - Strouhalova, Dana - Bobáľ, Pavel
PY - 2023
TI - Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley
JF - Journal of Agricultural and Food Chemistry
VL - 71
IS - 41
SP - 14825-14837
EP - 14825-14837
PB - AMER CHEMICAL SOC
SN - 00218561
KW - barley
KW - post-translationalmodification
KW - protein
KW - mass spectrometry
UR - https://pubs.acs.org/doi/10.1021/acs.jafc.3c00886
N2 - Post-translational modifications (PTMs) of biomacromolecules can be useful for understanding the processes by which a relatively small number of individual genes in a particular genome can generate enormous biological complexity in different organisms. The proteomes of barley and the brewing process were investigated by different techniques. However, their diverse and complex PTMs remain understudied. As standard analytical approaches have limitations, innovative analytical approaches need to be developed and applied in PTM studies. To make further progress in this field, it is necessary to specify the sites of modification, as well as to characterize individual isoforms with increased selectivity and sensitivity. This review summarizes advances in the PTM analysis of barley proteins, particularly those involving mass spectrometric detection. Our focus is on monitoring phosphorylation, glycation, and glycosylation, which critically influence functional behavior in metabolism and regulation in organisms.
ER -
BOBALOVA, Janette, Dana STROUHALOVA and Pavel BOBÁĽ. Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley. \textit{Journal of Agricultural and Food Chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2023, vol.~71, No~41, p.~14825-14837. ISSN~0021-8561. Available from: https://dx.doi.org/10.1021/acs.jafc.3c00886.
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