Development of lectin arrays for mapping the glycosylations of
proteins and cells

a 2023

Development of lectin arrays for mapping the glycosylations of proteins and cells

KOMÁREK, Jan, Marek KORSÁK, Lukáš FALTINEK and Michaela WIMMEROVÁ

Basic information

Original name

Development of lectin arrays for mapping the glycosylations of proteins and cells

Name in Czech

Vývoj lektinových mirkočipů pro mapování glykosylace proteinů a buněk

Authors

KOMÁREK, Jan (203 Czech Republic, belonging to the institution), Marek KORSÁK (703 Slovakia, belonging to the institution), Lukáš FALTINEK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

XXIInd Meeting of Biochemists and Molecular Biologists, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10609 Biochemical research methods

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/23:00132291

Organization unit

Faculty of Science

ISBN

978-80-280-0409-5

Keywords (in Czech)

lektiny; glykosylace; lektinové čipy

Keywords in English

lectins; glycosylation; lectin arrays

Změněno: 16/11/2023 09:30, Mgr. Jan Komárek, Ph.D.

Abstract

V originále

Saccharides are essential for life, and in addition to their traditional roles as structural and energy storage components, they play crucial roles in many recognition events, signaling, and communication processes [1]. The glycosylation of cells reflects the biological species, tissue, developmental and physiological state of the organism. Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis [2]. Lectin arrays are widely used tools for analyzing glycosylation of various samples. They utilize a panel of lectins (saccharide-binding proteins with distinct sugar specificities) immobilized onto a solid surface. Lectin arrays allow for rapid and high-throughput glycomic analysis with minimal sample consumption. They can be used for glycan profiling of purified glycoproteins, cell/tissue lysates, membrane vesicles, complex biological samples, prokaryotic and eukaryotic cells. This talk will present the development of a customized lectin array in the microscope slide format. The microarrays are prepared by non-contact piezo dispensing of lectins onto a functionalized glass slide and their immobilization using various surface chemistries (immobilization on epoxy groups, NHS/EDC coupling). Fluorescence measurement is used to detect the binding of the glycosylated samples. The fabricated lectin arrays will be used, e.g., to map the glycosylation changes at different stages of cell differentiation or under different physiopathological conditions. [1] Zhan X, Dravid G, Ye Z, Hammond H, Shamblott M, Gearhart J, Cheng L. Lancet. 2004 [2] Mazalovska M, Kouokam JC. Biomed Res Int. 2020.

Links

LM2023042, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministry of Education, Youth and Sports of the CR, CIISB - Czech Infrastructure for Integrative Structural Biology

Citovat

KOMÁREK, Jan, Marek KORSÁK, Lukáš FALTINEK and Michaela WIMMEROVÁ. Development of lectin arrays for mapping the glycosylations of proteins and cells. In XXIInd Meeting of Biochemists and Molecular Biologists. 2023. ISBN 978-80-280-0409-5.

@proceedings{2339924,
   author = {Komárek, Jan and Korsák, Marek and Faltinek, Lukáš and Wimmerová, Michaela},
   booktitle = {XXIInd Meeting of Biochemists and Molecular Biologists},
   keywords = {lectins; glycosylation; lectin arrays},
   language = {eng},
   isbn = {978-80-280-0409-5},
   title = {Development of lectin arrays for mapping the glycosylations of proteins and cells},
   year = {2023}
}

TY  - CONF
ID  - 2339924
AU  - Komárek, Jan - Korsák, Marek - Faltinek, Lukáš - Wimmerová, Michaela
PY  - 2023
TI  - Development of lectin arrays for mapping the glycosylations of proteins and cells
SN  - 9788028004095
KW  - lectins
KW  - glycosylation
KW  - lectin arrays
N2  - Saccharides are essential for life, and in addition to their traditional roles as structural and energy storage components, they play crucial roles in many recognition events, signaling, and communication processes [1]. The glycosylation of cells reflects the biological species, tissue, developmental and physiological state of the organism. Importantly, changes in glycosylation patterns were observed in various tumors, and the aberrant glycosylation is often associated with malignancy potential, tumor immune surveillance, and patients´ prognosis [2]. Lectin arrays are widely used tools for analyzing glycosylation of various samples. They utilize a panel of lectins (saccharide-binding proteins with distinct sugar specificities) immobilized onto a solid surface. Lectin arrays allow for rapid and high-throughput glycomic analysis with minimal sample consumption. They can be used for glycan profiling of purified glycoproteins, cell/tissue lysates, membrane vesicles, complex biological samples, prokaryotic and eukaryotic cells. This talk will present the development of a customized lectin array in the microscope slide format. The microarrays are prepared by non-contact piezo dispensing of lectins onto a functionalized glass slide and their immobilization using various surface chemistries (immobilization on epoxy groups, NHS/EDC coupling). Fluorescence measurement is used to detect the binding of the glycosylated samples. The fabricated lectin arrays will be used, e.g., to map the glycosylation changes at different stages of cell differentiation or under different physiopathological conditions. [1] Zhan X, Dravid G, Ye Z, Hammond H, Shamblott M, Gearhart J, Cheng L. Lancet. 2004 [2] Mazalovska M, Kouokam JC. Biomed Res Int. 2020.
ER  -

KOMÁREK, Jan, Marek KORSÁK, Lukáš FALTINEK and Michaela WIMMEROVÁ. Development of lectin arrays for mapping the glycosylations of proteins and cells. In \textit{XXIInd Meeting of Biochemists and Molecular Biologists}. 2023. ISBN~978-80-280-0409-5.

Detailed Information on Publication Record