ANTIPHLOGISTIC EFFECT OF POTENTILLA ARGENTEA EXTRACT IN A
DEXTRAN SULFATE-INDUCED COLITIS RAT MODEL

a 2023

ANTIPHLOGISTIC EFFECT OF POTENTILLA ARGENTEA EXTRACT IN A DEXTRAN SULFATE-INDUCED COLITIS RAT MODEL

SUCHÝ, Pavel, Marta CHALUPOVÁ, Alžběta KRUŽICOVÁ, Tomáš PARÁK, Lenka PARÁKOVÁ et. al.

Basic information

Original name

ANTIPHLOGISTIC EFFECT OF POTENTILLA ARGENTEA EXTRACT IN A DEXTRAN SULFATE-INDUCED COLITIS RAT MODEL

Name in Czech

ANTIFLOGISTICKÝ ÚČINEK POTENTILLA ARGENTEA V DEXTRAN SULFÁTEM INDUKOVANÉ KOLITIDĚ NA MODELU POTKANŮ

Authors

SUCHÝ, Pavel, Marta CHALUPOVÁ, Alžběta KRUŽICOVÁ, Tomáš PARÁK, Lenka PARÁKOVÁ, Gabriela KUZMÍNOVÁ, Michal BELEJKANIČ, Karel ŠMEJKAL, Jarmila KLUSÁKOVÁ and Alfred HERA

Edition

28th Interdisciplinary Toxicological Conference - Toxcon 2023, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Pharmacy

ISSN

Keywords in English

IBD, anti-inflammatory, histopathological examination, molecular-biological examination

Abstract

V originále

Potentilla argentea is one of the medicinal plants traditionally used for digestive problems and especially for diarrhea to relieve spasms and abdominal pain. The therapeutic effectiveness is based on the content of active substances such as flavonoids, tannins, choline, histidine and other substances. The anti-inflammatory effect of Potentilla argentea extract was evaluated in a dextran sodium sulfate (DSS)-induced colon inflammation model in rats. After ulcerative colitis induction, plant extract was administered per os every 24 h by gastric tube for 5 days. In the control group, only vehicle (water) was administered. The negative control consisted of intact animals without induced inflammation. After the final experiment, colon tissue samples were taken for histopathological examination. The extent of inflammation was determined using a semiquantitative method. At the same time, a molecular-biological examination was performed to determine the level of pro-inflammatory interleukin 6 (IL-6) and transforming growth factor beta 1 (TGF beta1) in the intestinal tissue. The conducted study demonstrated the usability of the selected model of experimentally induced colitis in laboratory rats (DSS model) for verifying the anti-inflammatory effect of plant extracts and its dose-dependent effect. The achieved results could be used in the therapy of chronic intestinal inflammation (IBD) and limiting the use of antibiotics in treating diarrheal diseases.

Citovat

SUCHÝ, Pavel, Marta CHALUPOVÁ, Alžběta KRUŽICOVÁ, Tomáš PARÁK, Lenka PARÁKOVÁ, Gabriela KUZMÍNOVÁ, Michal BELEJKANIČ, Karel ŠMEJKAL, Jarmila KLUSÁKOVÁ and Alfred HERA. ANTIPHLOGISTIC EFFECT OF POTENTILLA ARGENTEA EXTRACT IN A DEXTRAN SULFATE-INDUCED COLITIS RAT MODEL. In 28th Interdisciplinary Toxicological Conference - Toxcon 2023. 2023. ISSN 1337-6853.

@proceedings{2340061,
   author = {Suchý, Pavel and Chalupová, Marta and Kružicová, Alžběta and Parák, Tomáš and Paráková, Lenka and Kuzmínová, Gabriela and Belejkanič, Michal and Šmejkal, Karel and Klusáková, Jarmila and Hera, Alfred},
   booktitle = {28th Interdisciplinary Toxicological Conference - Toxcon 2023},
   keywords = {IBD, anti-inflammatory, histopathological examination, molecular-biological examination},
   language = {eng},
   title = {ANTIPHLOGISTIC EFFECT OF POTENTILLA ARGENTEA EXTRACT IN A DEXTRAN SULFATE-INDUCED COLITIS RAT MODEL},
   year = {2023}
}

TY  - CONF
ID  - 2340061
AU  - Suchý, Pavel - Chalupová, Marta - Kružicová, Alžběta - Parák, Tomáš - Paráková, Lenka - Kuzmínová, Gabriela - Belejkanič, Michal - Šmejkal, Karel - Klusáková, Jarmila - Hera, Alfred
PY  - 2023
TI  - ANTIPHLOGISTIC EFFECT OF POTENTILLA ARGENTEA EXTRACT IN A DEXTRAN SULFATE-INDUCED COLITIS RAT MODEL
KW  - IBD, anti-inflammatory, histopathological examination, molecular-biological examination
N2  - Potentilla argentea is one of the medicinal plants traditionally used for digestive problems and especially for diarrhea to relieve spasms and abdominal pain. The therapeutic effectiveness is based on the content of active substances such as flavonoids, tannins, choline, histidine and other substances. The anti-inflammatory effect of Potentilla argentea extract was evaluated in a dextran sodium sulfate (DSS)-induced colon inflammation model in rats. After ulcerative colitis induction, plant extract was administered per os every 24 h by gastric tube for 5 days. In the control group, only vehicle (water) was administered. The negative control consisted of intact animals without induced inflammation. After the final experiment, colon tissue samples were taken for histopathological examination. The extent of inflammation was determined using a semiquantitative method. At the same time, a molecular-biological examination was performed to determine the level of pro-inflammatory interleukin 6 (IL-6) and transforming growth factor beta 1 (TGF beta1) in the intestinal tissue. The conducted study demonstrated the usability of the selected model of experimentally induced colitis in laboratory rats (DSS model) for verifying the anti-inflammatory effect of plant extracts and its dose-dependent effect. The achieved results could be used in the therapy of chronic intestinal inflammation (IBD) and limiting the use of antibiotics in treating diarrheal diseases.
ER  -

SUCHÝ, Pavel, Marta CHALUPOVÁ, Alžběta KRUŽICOVÁ, Tomáš PARÁK, Lenka PARÁKOVÁ, Gabriela KUZMÍNOVÁ, Michal BELEJKANIČ, Karel ŠMEJKAL, Jarmila KLUSÁKOVÁ and Alfred HERA. ANTIPHLOGISTIC EFFECT OF POTENTILLA ARGENTEA EXTRACT IN A DEXTRAN SULFATE-INDUCED COLITIS RAT MODEL. In \textit{28th Interdisciplinary Toxicological Conference - Toxcon 2023}. 2023. ISSN~1337-6853.

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