Lectins are proteins that specifically bind carbohydrates and participate in recognition processes such as host-pathogen interaction or in various biological events such as cell proliferation, leukocyte migration, or apoptosis [1]. Lectin microarray is a technology that utilizes a collection of lectins immobilized on a solid support (usually a glass slide). It was developed for high-throughput analysis of glycosylation (e.g., of proteins or cells) and can also serve as a diagnostic tool [2]. This work was focused on cloning and recombinant production of mannose-specific lectin BanLec from banana [3], sialic acid-specific lectin ACG from mushroom Agrocybe cylindracea [4], and GalNAc-specific lectin HPA-I from the Roman snail [5]. In contrast to commercial lectins typically isolated from natural sources, lectins produced in E. coli expression system have several advantages (higher purity, lower costs, absence of glycosylation that might interfere with microarray results). All purified lectins will be used for lectin microarrays. [1] N. Sharon, Journal of Biological Chemistry, 2007 [2] S. Hu a D. T. Wong, Prot. Clin. Appl., 2009 [3] V. L. Koshte et al., Biochemical Journal, 1990 [4] F. Yagi et al., Glycoconjugate Journal, 1997 [5] J.-F. Sanchez et al., Journal of Biological Chemistry, 2006