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@proceedings{2340098, author = {Robotková, Kateřina and Pecůch, Václav and Komárek, Jan and Wimmerová, Michaela}, booktitle = {XXIInd Meeting of Biochemists and Molecular Biologists}, keywords = {Lectins; lectin arrays; BanLec; ACG; HPA}, language = {eng}, isbn = {978-80-280-0409-5}, title = {Production of lectins for lectin arrays}, year = {2023} }
TY - CONF ID - 2340098 AU - Robotková, Kateřina - Pecůch, Václav - Komárek, Jan - Wimmerová, Michaela PY - 2023 TI - Production of lectins for lectin arrays SN - 9788028004095 KW - Lectins KW - lectin arrays KW - BanLec KW - ACG KW - HPA N2 - Lectins are proteins that specifically bind carbohydrates and participate in recognition processes such as host-pathogen interaction or in various biological events such as cell proliferation, leukocyte migration, or apoptosis [1]. Lectin microarray is a technology that utilizes a collection of lectins immobilized on a solid support (usually a glass slide). It was developed for high-throughput analysis of glycosylation (e.g., of proteins or cells) and can also serve as a diagnostic tool [2]. This work was focused on cloning and recombinant production of mannose-specific lectin BanLec from banana [3], sialic acid-specific lectin ACG from mushroom Agrocybe cylindracea [4], and GalNAc-specific lectin HPA-I from the Roman snail [5]. In contrast to commercial lectins typically isolated from natural sources, lectins produced in E. coli expression system have several advantages (higher purity, lower costs, absence of glycosylation that might interfere with microarray results). All purified lectins will be used for lectin microarrays. [1] N. Sharon, Journal of Biological Chemistry, 2007 [2] S. Hu a D. T. Wong, Prot. Clin. Appl., 2009 [3] V. L. Koshte et al., Biochemical Journal, 1990 [4] F. Yagi et al., Glycoconjugate Journal, 1997 [5] J.-F. Sanchez et al., Journal of Biological Chemistry, 2006 ER -
ROBOTKOVÁ, Kateřina, Václav PECŮCH, Jan KOMÁREK and Michaela WIMMEROVÁ. Production of lectins for lectin arrays. In \textit{XXIInd Meeting of Biochemists and Molecular Biologists}. 2023. ISBN~978-80-280-0409-5.
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