ROBOTKOVÁ, Kateřina, Václav PECŮCH, Jan KOMÁREK and Michaela WIMMEROVÁ. Production of lectins for lectin arrays. In XXIInd Meeting of Biochemists and Molecular Biologists. 2023. ISBN 978-80-280-0409-5.
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Basic information
Original name Production of lectins for lectin arrays
Name in Czech Příprava lektinů pro lektinové čipy
Authors ROBOTKOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Václav PECŮCH (203 Czech Republic, belonging to the institution), Jan KOMÁREK (203 Czech Republic, belonging to the institution) and Michaela WIMMEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition XXIInd Meeting of Biochemists and Molecular Biologists, 2023.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10608 Biochemistry and molecular biology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/23:00132297
Organization unit Faculty of Science
ISBN 978-80-280-0409-5
Keywords (in Czech) Lektiny; lektinové čipy; BanLec; ACG; HPA
Keywords in English Lectins; lectin arrays; BanLec; ACG; HPA
Changed by Changed by: Bc. Kateřina Robotková, učo 505192. Changed: 16/11/2023 16:14.
Abstract
Lectins are proteins that specifically bind carbohydrates and participate in recognition processes such as host-pathogen interaction or in various biological events such as cell proliferation, leukocyte migration, or apoptosis [1]. Lectin microarray is a technology that utilizes a collection of lectins immobilized on a solid support (usually a glass slide). It was developed for high-throughput analysis of glycosylation (e.g., of proteins or cells) and can also serve as a diagnostic tool [2]. This work was focused on cloning and recombinant production of mannose-specific lectin BanLec from banana [3], sialic acid-specific lectin ACG from mushroom Agrocybe cylindracea [4], and GalNAc-specific lectin HPA-I from the Roman snail [5]. In contrast to commercial lectins typically isolated from natural sources, lectins produced in E. coli expression system have several advantages (higher purity, lower costs, absence of glycosylation that might interfere with microarray results). All purified lectins will be used for lectin microarrays. [1] N. Sharon, Journal of Biological Chemistry, 2007 [2] S. Hu a D. T. Wong, Prot. Clin. Appl., 2009 [3] V. L. Koshte et al., Biochemical Journal, 1990 [4] F. Yagi et al., Glycoconjugate Journal, 1997 [5] J.-F. Sanchez et al., Journal of Biological Chemistry, 2006
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