Rapid identification of pathogens in blood serum via Raman
tweezers in combination with advanced processing methods

J 2023

Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods

VACULÍK, Ondřej, Silvie BERNATOVÁ, Katarína REBROŠOVÁ, Ota SAMEK, Lukáš ŠILHAN et. al.

Basic information

Original name

Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods

Authors

VACULÍK, Ondřej (203 Czech Republic), Silvie BERNATOVÁ (203 Czech Republic), Katarína REBROŠOVÁ (703 Slovakia, belonging to the institution), Ota SAMEK (203 Czech Republic), Lukáš ŠILHAN (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution), Mojmír ŠERÝ (203 Czech Republic), Martin ŠILER (203 Czech Republic), Jan JEŽEK (203 Czech Republic) and Pavel ZEMÁNEK (203 Czech Republic)

Edition

Biomedical Optics Express, WASHINGTON, OPTICA PUBLISHING GROUP, 2023, 2156-7085

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.400 in 2022

RIV identification code

RIV/00216224:14110/23:00134679

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1364/BOE.503628

UT WoS

001123729400003

Keywords in English

Raman tweezers; blood serum; pathogens

Abstract

V originále

Pathogenic microbes contribute to several major global diseases that kill millions of people every year. Bloodstream infections caused by these microbes are associated with high morbidity and mortality rates, which are among the most common causes of hospitalizations. The search for the “Holy Grail” in clinical diagnostic microbiology, a reliable, accurate, low cost, real-time, and easy-to-use diagnostic method, is one of the essential issues in clinical practice. These very critical conditions can be met by Raman tweezers in combination with advanced analysis methods. Here, we present a proof-of-concept study based on Raman tweezers combined with spectral mixture analysis that allows for the identification of microbial strains directly from human blood serum without user intervention, thus eliminating the influence of a data analyst.

Links

NU21-05-00341, research and development project

Name: Pokročilé metody detekce patogenních mikroorganizmů a hodnocení účinku antimikrobiálních látek Ramanovou spektroskopií a dalšími metodami na úrovni molekul v optofluidních systémech

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

VACULÍK, Ondřej, Silvie BERNATOVÁ, Katarína REBROŠOVÁ, Ota SAMEK, Lukáš ŠILHAN, Filip RŮŽIČKA, Mojmír ŠERÝ, Martin ŠILER, Jan JEŽEK and Pavel ZEMÁNEK. Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods. Biomedical Optics Express. WASHINGTON: OPTICA PUBLISHING GROUP, 2023, vol. 14, No 12, p. 6410-6421. ISSN 2156-7085. Available from: https://dx.doi.org/10.1364/BOE.503628.

@article{2343978,
   author = {Vaculík, Ondřej and Bernatová, Silvie and Rebrošová, Katarína and Samek, Ota and Šilhan, Lukáš and Růžička, Filip and Šerý, Mojmír and Šiler, Martin and Ježek, Jan and Zemánek, Pavel},
   article_location = {WASHINGTON},
   article_number = {12},
   doi = {http://dx.doi.org/10.1364/BOE.503628},
   keywords = {Raman tweezers; blood serum; pathogens},
   language = {eng},
   issn = {2156-7085},
   journal = {Biomedical Optics Express},
   title = {Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods},
   url = {https://opg.optica.org/boe/fulltext.cfm?uri=boe-14-12-6410&id=542842#},
   volume = {14},
   year = {2023}
}

TY  - JOUR
ID  - 2343978
AU  - Vaculík, Ondřej - Bernatová, Silvie - Rebrošová, Katarína - Samek, Ota - Šilhan, Lukáš - Růžička, Filip - Šerý, Mojmír - Šiler, Martin - Ježek, Jan - Zemánek, Pavel
PY  - 2023
TI  - Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods
JF  - Biomedical Optics Express
VL  - 14
IS  - 12
SP  - 6410-6421
EP  - 6410-6421
PB  - OPTICA PUBLISHING GROUP
SN  - 21567085
KW  - Raman tweezers
KW  - blood serum
KW  - pathogens
UR  - https://opg.optica.org/boe/fulltext.cfm?uri=boe-14-12-6410&id=542842#
N2  - Pathogenic microbes contribute to several major global diseases that kill millions of people every year. Bloodstream infections caused by these microbes are associated with high morbidity and mortality rates, which are among the most common causes of hospitalizations. The search for the “Holy Grail” in clinical diagnostic microbiology, a reliable, accurate, low cost, real-time, and easy-to-use diagnostic method, is one of the essential issues in clinical practice. These very critical conditions can be met by Raman tweezers in combination with advanced analysis methods. Here, we present a proof-of-concept study based on Raman tweezers combined with spectral mixture analysis that allows for the identification of microbial strains directly from human blood serum without user intervention, thus eliminating the influence of a data analyst.
ER  -

VACULÍK, Ondřej, Silvie BERNATOVÁ, Katarína REBROŠOVÁ, Ota SAMEK, Lukáš ŠILHAN, Filip RŮŽIČKA, Mojmír ŠERÝ, Martin ŠILER, Jan JEŽEK and Pavel ZEMÁNEK. Rapid identification of pathogens in blood serum via Raman tweezers in combination with advanced processing methods. \textit{Biomedical Optics Express}. WASHINGTON: OPTICA PUBLISHING GROUP, 2023, vol.~14, No~12, p.~6410-6421. ISSN~2156-7085. Available from: https://dx.doi.org/10.1364/BOE.503628.

Detailed Information on Publication Record