Unravelling Tick-Borne Encephalitis Virus Neutralisation
Mechanisms with Cryo-EM

a 2023

Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM

NEPOVÍMOVÁ, Lucie, Tibor FÜZIK, Lenka ŠMERDOVÁ and Pavel PLEVKA

Basic information

Original name

Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM

Name in Czech

Odhalení mechanismů neutralizace viru klíšťové encefalitidy pomocí Cryo-EM

Authors

NEPOVÍMOVÁ, Lucie (203 Czech Republic, belonging to the institution), Tibor FÜZIK (703 Slovakia, belonging to the institution), Lenka ŠMERDOVÁ (203 Czech Republic, belonging to the institution) and Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

VBC Cryo-EM Symposium, 2023

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10607 Virology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

web symposia

RIV identification code

RIV/00216224:14740/23:00134306

Organization unit

Central European Institute of Technology

Keywords in English

TBEV; cryo-EM; antibodies

Abstract

V originále

Tick-borne encephalitis (TBE), caused by the tick-borne encephalitis virus (TBEV), is a severe disease that can lead to fatal central nervous system inflammation. Despite the availability of vaccines, vaccination rates in the most affected areas, such as the Czech Republic, are low. With TBE cases on the rise, the need for targeted treatments is growing. Antibodies have shown promise in a mouse model, where intravenous administration of TBEV-specific antibodies served as a treatment. However, our understanding of the molecular mechanisms behind TBEV neutralisation remains limited.To address this gap, we investigated the interactions between the TBEV Neudörfl strain and two neutralising mouse monoclonal antibodies, IC3 and A4, which bind to distinct domains of the TBEV envelope protein. We purified TBEV from infected tissue culture cells, mixed it with Fab fragments derived from the neutralising antibodies, and vitrified the samples on grids for cryo-electron microscopy. Using single-particle analysis, we solved the structures of the TBEV-Fab complexes from the collected micrographs.By deciphering the molecular basis of TBEV neutralisation by antibodies, we aim to gain insights into the significance of different epitopes on the viral surface. This knowledge may pave the way for the tailored design of therapeutic antibodies or more specific vaccines in the future.

Links

GX19-25982X, research and development project

Name: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Czech Science Foundation

Citovat

NEPOVÍMOVÁ, Lucie, Tibor FÜZIK, Lenka ŠMERDOVÁ and Pavel PLEVKA. Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM. In VBC Cryo-EM Symposium. 2023.

@proceedings{2344620,
   author = {Nepovímová, Lucie and Füzik, Tibor and Šmerdová, Lenka and Plevka, Pavel},
   booktitle = {VBC Cryo-EM Symposium},
   keywords = {TBEV; cryo-EM; antibodies},
   language = {eng},
   title = {Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM},
   url = {https://www.imp.ac.at/research/cryo-em-symposium},
   year = {2023}
}

TY  - CONF
ID  - 2344620
AU  - Nepovímová, Lucie - Füzik, Tibor - Šmerdová, Lenka - Plevka, Pavel
PY  - 2023
TI  - Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM
KW  - TBEV
KW  - cryo-EM
KW  - antibodies
UR  - https://www.imp.ac.at/research/cryo-em-symposium
N2  - Tick-borne encephalitis (TBE), caused by the tick-borne encephalitis virus (TBEV), is a severe disease that can lead to fatal central nervous system inflammation. Despite the availability of vaccines, vaccination rates in the most affected areas, such as the Czech Republic, are low. With TBE cases on the rise, the need for targeted treatments is growing. Antibodies have shown promise in a mouse model, where intravenous administration of TBEV-specific antibodies served as a treatment. However, our understanding of the molecular mechanisms behind TBEV neutralisation remains limited.To address this gap, we investigated the interactions between the TBEV Neudörfl strain and two neutralising mouse monoclonal antibodies, IC3 and A4, which bind to distinct domains of the TBEV envelope protein. We purified TBEV from infected tissue culture cells, mixed it with Fab fragments derived from the neutralising antibodies, and vitrified the samples on grids for cryo-electron microscopy. Using single-particle analysis, we solved the structures of the TBEV-Fab complexes from the collected micrographs.By deciphering the molecular basis of TBEV neutralisation by antibodies, we aim to gain insights into the significance of different epitopes on the viral surface. This knowledge may pave the way for the tailored design of therapeutic antibodies or more specific vaccines in the future.
ER  -

NEPOVÍMOVÁ, Lucie, Tibor FÜZIK, Lenka ŠMERDOVÁ and Pavel PLEVKA. Unravelling Tick-Borne Encephalitis Virus Neutralisation Mechanisms with Cryo-EM. In \textit{VBC Cryo-EM Symposium}. 2023.

Detailed Information on Publication Record