In vivo molecular biocompatibility of Calotropis gigentea
contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver
microgel hybrid with embryonic Danio rerio inferred via
intrinsic atomic physiological impacts

J 2023

In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts

JHA, Ealisha, Paritosh PATEL, Puja KUMARI, Krishn Kumar VERMA, Pritam Kumar PANDA et. al.

Basic information

Original name

In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts

Authors

JHA, Ealisha, Paritosh PATEL, Puja KUMARI (356 India, guarantor, belonging to the institution), Krishn Kumar VERMA, Pritam Kumar PANDA, Priti S. MOHANTY, Swadheena PATRO, Rajender S. VARMA, Yogendra Kumar MISHRA, Nagendra Kumar KAUSHIK, Mrutyunjay SUAR and Suresh K. VERMA

Edition

Journal of Environmental Chemical Engineering, Oxford, Elsevier, 2023, 2213-2929

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.700 in 2022

RIV identification code

RIV/00216224:14310/23:00132394

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.jece.2023.111183

UT WoS

001091697900001

Keywords in English

PNIPAM-co-sulphonic@AgNPs hybrid; Antibacterial activity; Zebrafish; in vivo biocompatibility

Abstract

V originále

The usage of silver nanoparticles (AgNPs) is expected to aggrandize for different ecological applications, owing to inimitable physical, chemical, and biological properties. The need of hour propels the quest for new technologies concerning eco-compatible synthesis of AgNPs and their hybrid forms with higher biocompatibility and maximum efficacy. This study proposes a novel ecofriendly synthesis of antibacterial Poly(N-isopropylacrylamide)-co-sulphonic-Silver (pNSAg) hybrid aided by the aqueous floral extract of Calotropis gigantea. and inquisite its higher mechanistic in vivo biocompatibility with zebrafish. Physiochemical charac-terization of pNSAg confirmed the hybridization of AgNPs with pNIPAM with thermo-sensitive size variation property as determined by dynamic light scattering, FESEM, and flow cytometry. Comparative antibacterial analysis showed concentration and temperature-dependent higher activity of pNSAg compared to AgNPs at 20 degrees C. In vivo biocompatibility investigation determined the LC50 of 112.3 mu g/mL for pNSAg compared to 51.2 mu g/mL for AgNPs with embryonic zebrafish. Mechanistic biocompatibility unraveled it as effect of induction of oxidative stress leading to apoptosis via interaction with metabolic proteins like he1a, Sod1, and p53. The study provided insight into in vivo biocompatibility of polymer-metal nanoparticles hybrid with an eco-compatible approach for their synthesis paving a pathway to ecological and biomedical applications.

Citovat

JHA, Ealisha, Paritosh PATEL, Puja KUMARI, Krishn Kumar VERMA, Pritam Kumar PANDA, Priti S. MOHANTY, Swadheena PATRO, Rajender S. VARMA, Yogendra Kumar MISHRA, Nagendra Kumar KAUSHIK, Mrutyunjay SUAR and Suresh K. VERMA. In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts. Journal of Environmental Chemical Engineering. Oxford: Elsevier, 2023, vol. 11, No 6, p. 1-17. ISSN 2213-2929. Available from: https://dx.doi.org/10.1016/j.jece.2023.111183.

@article{2345260,
   author = {Jha, Ealisha and Patel, Paritosh and Kumari, Puja and Verma, Krishn Kumar and Panda, Pritam Kumar and Mohanty, Priti S. and Patro, Swadheena and Varma, Rajender S. and Mishra, Yogendra Kumar and Kaushik, Nagendra Kumar and Suar, Mrutyunjay and Verma, Suresh K.},
   article_location = {Oxford},
   article_number = {6},
   doi = {http://dx.doi.org/10.1016/j.jece.2023.111183},
   keywords = {PNIPAM-co-sulphonic@AgNPs hybrid; Antibacterial activity; Zebrafish; in vivo biocompatibility},
   language = {eng},
   issn = {2213-2929},
   journal = {Journal of Environmental Chemical Engineering},
   title = {In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts},
   url = {https://www.sciencedirect.com/science/article/pii/S221334372301922X?via%3Dihub},
   volume = {11},
   year = {2023}
}

TY  - JOUR
ID  - 2345260
AU  - Jha, Ealisha - Patel, Paritosh - Kumari, Puja - Verma, Krishn Kumar - Panda, Pritam Kumar - Mohanty, Priti S. - Patro, Swadheena - Varma, Rajender S. - Mishra, Yogendra Kumar - Kaushik, Nagendra Kumar - Suar, Mrutyunjay - Verma, Suresh K.
PY  - 2023
TI  - In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts
JF  - Journal of Environmental Chemical Engineering
VL  - 11
IS  - 6
SP  - 1-17
EP  - 1-17
PB  - Elsevier
SN  - 22132929
KW  - PNIPAM-co-sulphonic@AgNPs hybrid
KW  - Antibacterial activity
KW  - Zebrafish
KW  - in vivo biocompatibility
UR  - https://www.sciencedirect.com/science/article/pii/S221334372301922X?via%3Dihub
N2  - The usage of silver nanoparticles (AgNPs) is expected to aggrandize for different ecological applications, owing to inimitable physical, chemical, and biological properties. The need of hour propels the quest for new technologies concerning eco-compatible synthesis of AgNPs and their hybrid forms with higher biocompatibility and maximum efficacy. This study proposes a novel ecofriendly synthesis of antibacterial Poly(N-isopropylacrylamide)-co-sulphonic-Silver (pNSAg) hybrid aided by the aqueous floral extract of Calotropis gigantea. and inquisite its higher mechanistic in vivo biocompatibility with zebrafish. Physiochemical charac-terization of pNSAg confirmed the hybridization of AgNPs with pNIPAM with thermo-sensitive size variation property as determined by dynamic light scattering, FESEM, and flow cytometry. Comparative antibacterial analysis showed concentration and temperature-dependent higher activity of pNSAg compared to AgNPs at 20 degrees C. In vivo biocompatibility investigation determined the LC50 of 112.3 mu g/mL for pNSAg compared to 51.2 mu g/mL for AgNPs with embryonic zebrafish. Mechanistic biocompatibility unraveled it as effect of induction of oxidative stress leading to apoptosis via interaction with metabolic proteins like he1a, Sod1, and p53. The study provided insight into in vivo biocompatibility of polymer-metal nanoparticles hybrid with an eco-compatible approach for their synthesis paving a pathway to ecological and biomedical applications.
ER  -

JHA, Ealisha, Paritosh PATEL, Puja KUMARI, Krishn Kumar VERMA, Pritam Kumar PANDA, Priti S. MOHANTY, Swadheena PATRO, Rajender S. VARMA, Yogendra Kumar MISHRA, Nagendra Kumar KAUSHIK, Mrutyunjay SUAR and Suresh K. VERMA. In vivo molecular biocompatibility of Calotropis gigentea contrived smart Poly(N-isopropylacrylamide)-co-sulphonic-Silver microgel hybrid with embryonic Danio rerio inferred via intrinsic atomic physiological impacts. \textit{Journal of Environmental Chemical Engineering}. Oxford: Elsevier, 2023, vol.~11, No~6, p.~1-17. ISSN~2213-2929. Available from: https://dx.doi.org/10.1016/j.jece.2023.111183.

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