DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ladislav HOUŠŤAVA and Pavel HANINEC. Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors. General Physiology and Biophysics. Bratislava: Veda, vol. 18, No 1, p. 63-65. ISSN 0231-5882. 1999.
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Basic information
Original name Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
Name in Czech Imunohistochemická analýza lamininu v acelulárním nervovém štěpu
Authors DUBOVÝ, Petr (203 Czech Republic, guarantor), Ivana SVÍŽENSKÁ (203 Czech Republic), Radim JANČÁLEK (203 Czech Republic), Ilona KLUSÁKOVÁ (203 Czech Republic), Ladislav HOUŠŤAVA (203 Czech Republic) and Pavel HANINEC (203 Czech Republic).
Edition General Physiology and Biophysics, Bratislava, Veda, 1999, 0231-5882.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30000 3. Medical and Health Sciences
Country of publisher Slovakia
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.400
RIV identification code RIV/00216224:14110/99:00001800
Organization unit Faculty of Medicine
Keywords in English Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration
Tags acellular nerve graft, Integrins, Laminin-1, Schwann cell migration
Changed by Changed by: prof. RNDr. Petr Dubový, CSc., učo 698. Changed: 17/6/2009 12:15.
Abstract
The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.
Abstract (in Czech)
Imunohistochemická detekce lamininu-1, collagen-IV, a6 a b1 řetězce integrinů byla studována v acelulárním segmentu nervu
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