Immunohistochemical localization of laminin-1 in the acellular
nerve grafts is associated with migrating Schwann cells which
display corresponding integrin receptors

J 1999

Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ladislav HOUŠŤAVA et. al.

Basic information

Original name

Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors

Name in Czech

Imunohistochemická analýza lamininu v acelulárním nervovém štěpu

Authors

DUBOVÝ, Petr (203 Czech Republic, guarantor), Ivana SVÍŽENSKÁ (203 Czech Republic), Radim JANČÁLEK (203 Czech Republic), Ilona KLUSÁKOVÁ (203 Czech Republic), Ladislav HOUŠŤAVA (203 Czech Republic) and Pavel HANINEC (203 Czech Republic)

Edition

General Physiology and Biophysics, Bratislava, Veda, 1999, 0231-5882

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.400

RIV identification code

RIV/00216224:14110/99:00001800

Organization unit

Faculty of Medicine

Keywords in English

Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration

Abstract

ORIG CZ

V originále

The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.

In Czech

Imunohistochemická detekce lamininu-1, collagen-IV, a6 a b1 řetězce integrinů byla studována v acelulárním segmentu nervu

Citovat

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ladislav HOUŠŤAVA and Pavel HANINEC. Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors. General Physiology and Biophysics. Bratislava: Veda, 1999, vol. 18, No 1, p. 63-65. ISSN 0231-5882.

@article{234931,
   author = {Dubový, Petr and Svíženská, Ivana and Jančálek, Radim and Klusáková, Ilona and Houšťava, Ladislav and Haninec, Pavel},
   article_location = {Bratislava},
   article_number = {1},
   keywords = {Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration},
   language = {eng},
   issn = {0231-5882},
   journal = {General Physiology and Biophysics},
   title = {Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors},
   volume = {18},
   year = {1999}
}

TY  - JOUR
ID  - 234931
AU  - Dubový, Petr - Svíženská, Ivana - Jančálek, Radim - Klusáková, Ilona - Houšťava, Ladislav - Haninec, Pavel
PY  - 1999
TI  - Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
JF  - General Physiology and Biophysics
VL  - 18
IS  - 1
SP  - 63
EP  - 63
PB  - Veda
SN  - 02315882
KW  - Acellular nerve graft
KW  - Integrins
KW  - Laminin-1
KW  - Schwann cell migration
N2  - The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.
ER  -

DUBOVÝ, Petr, Ivana SVÍŽENSKÁ, Radim JANČÁLEK, Ilona KLUSÁKOVÁ, Ladislav HOUŠŤAVA and Pavel HANINEC. Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors. \textit{General Physiology and Biophysics}. Bratislava: Veda, 1999, vol.~18, No~1, p.~63-65. ISSN~0231-5882.

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