Plastic response of macrophages to metal ions and nanoparticles
in time mimicking metal implant body environment

J 2024

Plastic response of macrophages to metal ions and nanoparticles in time mimicking metal implant body environment

NAVRÁTILOVÁ, Polina; Jan EMMER; Tomáš TOMÁŠ; Luděk RYBA; Jan BURDA et. al.

Basic information

Original name

Plastic response of macrophages to metal ions and nanoparticles in time mimicking metal implant body environment

Authors

NAVRÁTILOVÁ, Polina (643 Russian Federation, belonging to the institution); Jan EMMER (203 Czech Republic); Tomáš TOMÁŠ (203 Czech Republic, guarantor); Luděk RYBA (203 Czech Republic); Jan BURDA (203 Czech Republic); Tomáš LOJA (703 Slovakia, belonging to the institution); Jana VEVERKOVÁ (203 Czech Republic, belonging to the institution); Lucie VÁLKOVÁ (203 Czech Republic, belonging to the institution) and Monika PÁVKOVÁ GOLDBERGOVÁ (203 Czech Republic, belonging to the institution)

Edition

Environmental Science and Pollution Research, HEIDELBERG, SPRINGER HEIDELBERG, 2024, 0944-1344

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30404 Biomaterials

Country of publisher

Germany

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 5.800 in 2022

RIV identification code

RIV/00216224:14110/24:00135299

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s11356-023-31430-7

UT WoS

001125959900001

EID Scopus

2-s2.0-85182954892

Keywords in English

Metal toxicity; Implant debris; Nanoparticles; Metal ions; Macrophage; Polarization

Abstract

V originále

The paradigm of using metal biomaterials could be viewed from two sides — treatment of wide spectrum of degenerative diseases, and debris release from materials. After implant insertion, metal nanoparticles (NPs) and ions are released not only upon the first contact with cells/tissues, but in continual manner, which is immediately recognized by immune cells. In this work, the effects of metal nanoparticles (TiO2, Ni) and ions (Ni2+, Co2+, Cr3+, Mo6+) on primary human M0 macrophages from the blood samples of osteoarthritic patients undergoing total arthroplasty were studied in order to monitor immunomodulatory effects on the cells in a real-time format. The highest NiNPs concentration of 10 µg/ml had no effect on any of macrophage parameters, while the Ni2+ ions cytotoxicity limit for the cells is 0.5 mM. The cytotoxic effects of higher Ni2+ concentration revealed mitochondrial network fragmentation leading to mitochondrial dysfunction, accompanied by increased lysosomal activity and changes in pro-apoptotic markers. The suppression of M2 cell formation ability was connected to presence of Ni2+ ions (0.5 mM) and TiO2NPs (10 µg/ml). The immunomodulatory effect of Mo6+ ions, controversially, inhibit the formation of the cells with M1 phenotype and potentiate the thread-like shape M2s with increased chaotic cell movement. To summarize, metal toxicity depends on the debris form. Both, metal ions and nanoparticles affect macrophage size, morphological and functional parameters, but the effect of ions is more complex and likely more harmful, which has potential impact on healing and determines post-implantation reactions.

Links

MUNI/A/1370/2022, interní kód MU

Name: Patofyziologie vybraných komplexních nemocí od molekulární do systémovou úroveň

Investor: Masaryk University, Pathophysiology of selected complex diseases from molecular to systemic level

MUNI/A/1587/2023, interní kód MU

Name: Patofyziologie vybraných komplexních nemocí od molekulární do systémovou úroveň

Investor: Masaryk University, Pathophysiology of selected complex diseases from molecular to systemic level

NU20-08-00149, research and development project

Name: Multicentrické hodnocení hypersenzitivní reakce u pacientů indikovaných k totální náhradě kloubu včetně hodnocení důvodů reimplanace

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Cite

NAVRÁTILOVÁ, Polina; Jan EMMER; Tomáš TOMÁŠ; Luděk RYBA; Jan BURDA; Tomáš LOJA; Jana VEVERKOVÁ; Lucie VÁLKOVÁ and Monika PÁVKOVÁ GOLDBERGOVÁ. Plastic response of macrophages to metal ions and nanoparticles in time mimicking metal implant body environment. Environmental Science and Pollution Research. HEIDELBERG: SPRINGER HEIDELBERG, 2024, vol. 31, No 3, p. 4111-4129. ISSN 0944-1344. Available from: https://dx.doi.org/10.1007/s11356-023-31430-7.

@article{2351117,
   author = {Navrátilová, Polina and Emmer, Jan and Tomáš, Tomáš and Ryba, Luděk and Burda, Jan and Loja, Tomáš and Veverková, Jana and Válková, Lucie and Pávková Goldbergová, Monika},
   article_location = {HEIDELBERG},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s11356-023-31430-7},
   keywords = {Metal toxicity; Implant debris; Nanoparticles; Metal ions; Macrophage; Polarization},
   language = {eng},
   issn = {0944-1344},
   journal = {Environmental Science and Pollution Research},
   title = {Plastic response of macrophages to metal ions and nanoparticles in time mimicking metal implant body environment},
   url = {https://link.springer.com/article/10.1007/s11356-023-31430-7},
   volume = {31},
   year = {2024}
}

TY  - JOUR
ID  - 2351117
AU  - Navrátilová, Polina - Emmer, Jan - Tomáš, Tomáš - Ryba, Luděk - Burda, Jan - Loja, Tomáš - Veverková, Jana - Válková, Lucie - Pávková Goldbergová, Monika
PY  - 2024
TI  - Plastic response of macrophages to metal ions and nanoparticles in time mimicking metal implant body environment
JF  - Environmental Science and Pollution Research
VL  - 31
IS  - 3
SP  - 4111-4129
EP  - 4111-4129
PB  - SPRINGER HEIDELBERG
SN  - 09441344
KW  - Metal toxicity
KW  - Implant debris
KW  - Nanoparticles
KW  - Metal ions
KW  - Macrophage
KW  - Polarization
UR  - https://link.springer.com/article/10.1007/s11356-023-31430-7
N2  - The paradigm of using metal biomaterials could be viewed from two sides — treatment of wide spectrum of degenerative diseases, and debris release from materials. After implant insertion, metal nanoparticles (NPs) and ions are released not only upon the first contact with cells/tissues, but in continual manner, which is immediately recognized by immune cells. In this work, the effects of metal nanoparticles (TiO2, Ni) and ions (Ni2+, Co2+, Cr3+, Mo6+) on primary human M0 macrophages from the blood samples of osteoarthritic patients undergoing total arthroplasty were studied in order to monitor immunomodulatory effects on the cells in a real-time format. The highest NiNPs concentration of 10 µg/ml had no effect on any of macrophage parameters, while the Ni2+ ions cytotoxicity limit for the cells is 0.5 mM. The cytotoxic effects of higher Ni2+ concentration revealed mitochondrial network fragmentation leading to mitochondrial dysfunction, accompanied by increased lysosomal activity and changes in pro-apoptotic markers. The suppression of M2 cell formation ability was connected to presence of Ni2+ ions (0.5 mM) and TiO2NPs (10 µg/ml). The immunomodulatory effect of Mo6+ ions, controversially, inhibit the formation of the cells with M1 phenotype and potentiate the thread-like shape M2s with increased chaotic cell movement. To summarize, metal toxicity depends on the debris form. Both, metal ions and nanoparticles affect macrophage size, morphological and functional parameters, but the effect of ions is more complex and likely more harmful, which has potential impact on healing and determines post-implantation reactions.
ER  -

NAVRÁTILOVÁ, Polina; Jan EMMER; Tomáš TOMÁŠ; Luděk RYBA; Jan BURDA; Tomáš LOJA; Jana VEVERKOVÁ; Lucie VÁLKOVÁ and Monika PÁVKOVÁ GOLDBERGOVÁ. Plastic response of macrophages to metal ions and nanoparticles in time mimicking metal implant body environment. \textit{Environmental Science and Pollution Research}. HEIDELBERG: SPRINGER HEIDELBERG, 2024, vol.~31, No~3, p.~4111-4129. ISSN~0944-1344. Available from: https://dx.doi.org/10.1007/s11356-023-31430-7.

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