ŘÍHA, Karel, Jiří FAJKUS, Jiří ŠIROKÝ and Boris VYSKOT. Developmenthal control of telomere lengths and telomerase activity in plants. In 1st European workshop. 1st ed. Germany: 1st European workshop, 1998, p. -, 1 pp.
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Basic information
Original name Developmenthal control of telomere lengths and telomerase activity in plants
Authors ŘÍHA, Karel (203 Czech Republic), Jiří FAJKUS (203 Czech Republic, guarantor), Jiří ŠIROKÝ (203 Czech Republic) and Boris VYSKOT.
Edition 1. vyd. Germany, 1st European workshop, p. -, 1 pp. 1998.
Publisher 1st European workshop
Other information
Original language English
Type of outcome Proceedings paper
Field of Study Genetics and molecular biology
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/98:00002184
Organization unit Faculty of Science
Keywords in English telomere lengths; telomerase activity
Tags telomerase activity, telomere lengths
Changed by Changed by: prof. RNDr. Jiří Fajkus, CSc., učo 28574. Changed: 25/6/2008 08:30.
Abstract
Telomere lengths and telomerase activity were studied during the development of a model dioecious plant, Melandrium album (syn Silene latifolia). Telomeric DNA consisted of Arabidopsis-type TTTAGGG tandem repeats. The terminal positions of these repeats were confirmed by both Bal31 exonuclease degradation and in situ hybridization. Analysis of terminal restriction fragments in different tissues and ontogenetic stages showed that telomere lengths are stabilized precisely and do not change during plant growth and development. Telomerase activity tested by using a semiquantitative telomerase repeat amplification protocol correlated with cell proliferation in the tissues analyzed.
Links
MSM 143100008, plan (intention)Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
VS97032, research and development projectName: Analýza biologicky významných molekulárních komplexů
Investor: Ministry of Education, Youth and Sports of the CR, Analysis of biologically important molecular complexes
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