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@proceedings{2351921, author = {Bezdíček Králová, Stanislava and Flieder, Mathias and Chen, Songcan and Spacek, Peter and Bezdíček, Matěj and Dufková, Kristýna and Zehl, Martin and Zotchev, Sergey and Rattei, Thomas and Loy, Alexander}, booktitle = {10th FEMS Congress of European Microbiologists (FEMS 2023)}, keywords = {Antarctica; biosynthetic potential; novel antimicrobials; Streptomyces; Actinokineospora; genetic manipulations}, language = {eng}, title = {Untapped biosynthetic potential of Antarctic soil bacteria}, year = {2023} }
TY - CONF ID - 2351921 AU - Bezdíček Králová, Stanislava - Flieder, Mathias - Chen, Songcan - Spacek, Peter - Bezdíček, Matěj - Dufková, Kristýna - Zehl, Martin - Zotchev, Sergey - Rattei, Thomas - Loy, Alexander PY - 2023 TI - Untapped biosynthetic potential of Antarctic soil bacteria KW - Antarctica KW - biosynthetic potential KW - novel antimicrobials KW - Streptomyces KW - Actinokineospora KW - genetic manipulations N2 - Background: The growing problem of antimicrobial resistance motivated scientists to re-explore natural sources of bioactive secondary metabolites. Antarctica represents an extreme environment colonized by bacteria with unique adaptation mechanisms allowing them to thrive under harsh conditions. Such adaptations include production of secondary metabolites to inhibit competitors or sustain abiotic stresses, which predestines these microbes as source of natural products for biomedical use. Objectives: The aim of this work was to recover novel bacterial taxa from Antarctic soils to access the biosynthetic potential hidden in yet uncultivated bacteria. The main objective is the activation of silent biosynthetic gene clusters enabling discovery of novel secondary metabolites, mainly through co-cultivation strategies. Methods: Three isolation methods (pre-selection of spore-forming bacteria, low-nutrient and soil-extract based media) were applied to recover novel bacteria from Antarctic soils, predominantly targeting phyla with high biosynthetic potential such as Actinobacteriota, Proteobacteria and Firmicutes. Activation of silent biosynthetic gene clusters was attempted through targeted cultivation and co-cultivation. Screening for bioactive molecules and evaluation of their novelty was achieved by application of genomics, metabolomics, and bioactivity testing. Results: A collection of 917 isolates was established. Recovered isolates were associated with four bacterial phyla including 77 isolates of novel species. Proteobacteria and Actinobacteriota represented the most abundant phyla. Specific media stimulated biosynthesis of several unknown natural products. Eight strains produced antimicrobial compounds against resistant and multidrug-resistant bacterial and fungal pathogens. Importantly, metabolomic profiling indicated that these strains produced several new secondary metabolites, which may be responsible for the observed antimicrobial activities. ER -
BEZDÍČEK KRÁLOVÁ, Stanislava, Mathias FLIEDER, Songcan CHEN, Peter SPACEK, Matěj BEZDÍČEK, Kristýna DUFKOVÁ, Martin ZEHL, Sergey ZOTCHEV, Thomas RATTEI and Alexander LOY. Untapped biosynthetic potential of Antarctic soil bacteria. In \textit{10th FEMS Congress of European Microbiologists (FEMS 2023)}. 2023.
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