BEZDÍČEK KRÁLOVÁ, Stanislava, Mathias FLIEDER, Songcan CHEN, Peter SPACEK, Natalia STANIC, Matěj BEZDÍČEK, Martin ZEHL, Sergey ZOTCHEV, Thomas RATTEI and Alexander LOY. Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island. In Students in Polar and Alpine Research Conference (SPARC 2023). 2023.
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Basic information
Original name Exploring the Icy Treasures: Biosynthetic potential of Antarctic bacteria from James Ross Island.
Authors BEZDÍČEK KRÁLOVÁ, Stanislava (703 Slovakia, guarantor, belonging to the institution), Mathias FLIEDER (40 Austria), Songcan CHEN (156 China), Peter SPACEK (703 Slovakia), Natalia STANIC (688 Serbia), Matěj BEZDÍČEK (203 Czech Republic), Martin ZEHL (40 Austria), Sergey ZOTCHEV (643 Russian Federation), Thomas RATTEI (276 Germany) and Alexander LOY (276 Germany).
Edition Students in Polar and Alpine Research Conference (SPARC 2023), 2023.
Other information
Original language English
Type of outcome Presentations at conferences
Field of Study 10606 Microbiology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/23:00132600
Organization unit Faculty of Science
Keywords (in Czech) Antarktida; biosynteticky potencial; nové antimikrobiální látky; Streptomyces; ctinokineospora; genetické manipulace
Keywords in English Antarctica; biosynthetic potential; novel antimicrobials; Streptomyces; Actinokineospora; genetic manipulations
Tags International impact, Reviewed
Changed by Changed by: RNDr. Stanislava Bezdíček Králová, Ph.D., učo 357877. Changed: 20/12/2023 10:03.
Abstract
Background: The growing problem of antimicrobial resistance motivated scientists to re-explore natural sources of bioactive secondary metabolites. Antarctica represents an extreme environment colonized by bacteria with unique adaptation mechanisms allowing them to thrive under harsh conditions. Such adaptations include production of secondary metabolites to inhibit competitors or sustain abiotic stresses, which predestines these microbes as source of natural products for biomedical use. Objectives: The aim of this work was to recover novel bacterial taxa from Antarctic soils to access the biosynthetic potential hidden in yet uncultivated bacteria. The main objective is the activation of silent biosynthetic gene clusters enabling discovery of novel secondary metabolites, mainly through co-cultivation strategies. Methods: Three isolation methods (pre-selection of spore-forming bacteria, low-nutrient and soil-extract based media) were applied to recover novel bacteria from Antarctic soils, predominantly targeting phyla with high biosynthetic potential such as Actinobacteriota, Proteobacteria and Firmicutes. Activation of silent biosynthetic gene clusters was attempted through targeted cultivation and co-cultivation. Screening for bioactive molecules and evaluation of their novelty was achieved by application of genomics, metabolomics, and bioactivity testing. Results: A collection of 917 isolates was established. Recovered isolates were associated with four bacterial phyla including 77 isolates of novel species. Proteobacteria and Actinobacteriota represented the most abundant phyla. Specific media stimulated biosynthesis of several unknown natural products. Eight strains produced antimicrobial compounds against resistant and multidrug-resistant bacterial and fungal pathogens. Importantly, metabolomic profiling indicated that these strains produced several new secondary metabolites, which may be responsible for the observed antimicrobial activities.
Links
LM2015078, research and development projectName: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)
Investor: Ministry of Education, Youth and Sports of the CR
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