A scoring system for histological evaluation of skin during
Staphylococcus aureus infections

J 2024

A scoring system for histological evaluation of skin during Staphylococcus aureus infections

MAKOVICKÝ, Peter, Edita JEKLOVÁ, Břetislav LIPOVÝ, Filip RAŠKA, Martin KNOZ et. al.

Basic information

Original name

A scoring system for histological evaluation of skin during Staphylococcus aureus infections

Authors

MAKOVICKÝ, Peter (703 Slovakia), Edita JEKLOVÁ (203 Czech Republic), Břetislav LIPOVÝ (203 Czech Republic, belonging to the institution), Filip RAŠKA (203 Czech Republic, belonging to the institution), Martin KNOZ (203 Czech Republic, belonging to the institution), Rea JAROSOVA (203 Czech Republic), Šárka KOBZOVÁ (203 Czech Republic), Lukáš VACEK (203 Czech Republic, belonging to the institution), Adam NOREK (203 Czech Republic) and Lubomír JANDA (203 Czech Republic)

Edition

Biologia, New York, Springer, 2024, 0006-3088

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30303 Infectious Diseases

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.500 in 2022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s11756-023-01567-4

UT WoS

001105224900001

Keywords in English

Bacterial infection; Histology; MRSA; Non-healing wounds; S. aureus

Abstract

V originále

The histological evaluation of skin changes during Staphylococcus aureus (S. aureus) infection requires an exact approach with an accurate definition of individual changes and their parameters, including a classification score facilitating comparison among experiments. To propose methods and procedures for the evaluation of histological changes in skin infected by S. aureus strains in experimental pig models. Four pigs were included in the experiment. In the dorsal region of each pig, 10 wounds per pig (5 x 5 cm, 2 cm spacing) were created. Wounds in the individual pigs were inoculated with three different strains of methicillin- (oxacillin) and amoxicillin-resistant S. aureus at concentrations of 2 x 10(9)-5 x 10(9) CFU/ml. One pig was not inoculated and served as control. Subsequently, samples were collected from each wound on Days 3, 7, 10, ad 14 after inoculation processed by frozen section technique and the samples were stained with hematoxylin-eosin. Classification scores of selected parameters were determined and an average classification index was established at the end of the experiment. Histologically, granulomatous arrangement and superficial re-epithelialization were observed in the wounds of all animals. Compared to control, S. aureus infection documents slow healing of skin wounds with impaired epidermal formation (0.88 infected vs 1.45 control), re-epithelialization (1.25 vs 2.08), edema in the dermis (1.50 vs 2.67), inflammation in the dermis (0.50 vs 1.69), fibrosis in the dermis (4 vs 2.56), fibrosis in the hypodermis (4 vs 3.17) and, finally, necrosis in the dermis (1 vs 2.2). The results in the scores for scabs (1.25 vs 1.42), dermis (2.63 vs 2.67), and, finally, necrosis in the hypodermis (0.25 vs 0.75) did not differ among groups. The proposed methodological approach allowed quantification of histological changes and we recommend employing this scoring system in other studies evaluating the healing of skin wounds.

Links

NU20-05-00166, research and development project

Name: Nové přístupy k hojení infikovaných ran pomocí hydrogelů z přírodní pryskyřice Gum Karaya modifikovaných inovativními komponenty cílenými na multirezistentní kmeny bakterií (Acronym: NPHIRPHGK)

Investor: Ministry of Health of the CR, Subprogram 1 - standard

NU22-05-00475, research and development project

Name: Léčba infekcí způsobených multirezistentními kmeny bakterií pomocí nových antibakteriálních přípravků založených na katelicidinových antimikrobiálních peptidech

Investor: Ministry of Health of the CR, Subprogram 1 - standard

Citovat

MAKOVICKÝ, Peter, Edita JEKLOVÁ, Břetislav LIPOVÝ, Filip RAŠKA, Martin KNOZ, Rea JAROSOVA, Šárka KOBZOVÁ, Lukáš VACEK, Adam NOREK and Lubomír JANDA. A scoring system for histological evaluation of skin during Staphylococcus aureus infections. Biologia. New York: Springer, 2024, vol. 79, No 3, p. 917-925. ISSN 0006-3088. Available from: https://dx.doi.org/10.1007/s11756-023-01567-4.

@article{2352217,
   author = {Makovický, Peter and Jeklová, Edita and Lipový, Břetislav and Raška, Filip and Knoz, Martin and Jarosova, Rea and Kobzová, Šárka and Vacek, Lukáš and Norek, Adam and Janda, Lubomír},
   article_location = {New York},
   article_number = {3},
   doi = {http://dx.doi.org/10.1007/s11756-023-01567-4},
   keywords = {Bacterial infection; Histology; MRSA; Non-healing wounds; S. aureus},
   language = {eng},
   issn = {0006-3088},
   journal = {Biologia},
   title = {A scoring system for histological evaluation of skin during Staphylococcus aureus infections},
   url = {https://link.springer.com/article/10.1007/s11756-023-01567-4},
   volume = {79},
   year = {2024}
}

TY  - JOUR
ID  - 2352217
AU  - Makovický, Peter - Jeklová, Edita - Lipový, Břetislav - Raška, Filip - Knoz, Martin - Jarosova, Rea - Kobzová, Šárka - Vacek, Lukáš - Norek, Adam - Janda, Lubomír
PY  - 2024
TI  - A scoring system for histological evaluation of skin during Staphylococcus aureus infections
JF  - Biologia
VL  - 79
IS  - 3
SP  - 917-925
EP  - 917-925
PB  - Springer
SN  - 00063088
KW  - Bacterial infection
KW  - Histology
KW  - MRSA
KW  - Non-healing wounds
KW  - S. aureus
UR  - https://link.springer.com/article/10.1007/s11756-023-01567-4
N2  - The histological evaluation of skin changes during Staphylococcus aureus (S. aureus) infection requires an exact approach with an accurate definition of individual changes and their parameters, including a classification score facilitating comparison among experiments. To propose methods and procedures for the evaluation of histological changes in skin infected by S. aureus strains in experimental pig models. Four pigs were included in the experiment. In the dorsal region of each pig, 10 wounds per pig (5 x 5 cm, 2 cm spacing) were created. Wounds in the individual pigs were inoculated with three different strains of methicillin- (oxacillin) and amoxicillin-resistant S. aureus at concentrations of 2 x 10(9)-5 x 10(9) CFU/ml. One pig was not inoculated and served as control. Subsequently, samples were collected from each wound on Days 3, 7, 10, ad 14 after inoculation processed by frozen section technique and the samples were stained with hematoxylin-eosin. Classification scores of selected parameters were determined and an average classification index was established at the end of the experiment. Histologically, granulomatous arrangement and superficial re-epithelialization were observed in the wounds of all animals. Compared to control, S. aureus infection documents slow healing of skin wounds with impaired epidermal formation (0.88 infected vs 1.45 control), re-epithelialization (1.25 vs 2.08), edema in the dermis (1.50 vs 2.67), inflammation in the dermis (0.50 vs 1.69), fibrosis in the dermis (4 vs 2.56), fibrosis in the hypodermis (4 vs 3.17) and, finally, necrosis in the dermis (1 vs 2.2). The results in the scores for scabs (1.25 vs 1.42), dermis (2.63 vs 2.67), and, finally, necrosis in the hypodermis (0.25 vs 0.75) did not differ among groups. The proposed methodological approach allowed quantification of histological changes and we recommend employing this scoring system in other studies evaluating the healing of skin wounds.
ER  -

MAKOVICKÝ, Peter, Edita JEKLOVÁ, Břetislav LIPOVÝ, Filip RAŠKA, Martin KNOZ, Rea JAROSOVA, Šárka KOBZOVÁ, Lukáš VACEK, Adam NOREK and Lubomír JANDA. A scoring system for histological evaluation of skin during Staphylococcus aureus infections. \textit{Biologia}. New York: Springer, 2024, vol.~79, No~3, p.~917-925. ISSN~0006-3088. Available from: https://dx.doi.org/10.1007/s11756-023-01567-4.

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