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@article{2353802,
author = {AckermannandGäumann, Rahel and Brêchet, Arthur and Smetana, Jan and Salát, Jiří and Lienhard, Reto and Croxatto, Antony and Polcarová, Petra and Chlíbek, Roman and Růžek, Daniel},
article_location = {Amsterdam},
article_number = {December},
doi = {http://dx.doi.org/10.1016/j.jviromet.2023.114831},
keywords = {Tick-borne encephalitis; Antibody; Non-structural protein 1; NS1; Vaccination; Flavivirus},
language = {eng},
issn = {0166-0934},
journal = {Journal of Virological Methods},
title = {Vaccination against tick-borne encephalitis elicits a detectable NS1 IgG antibody response},
url = {https://www.sciencedirect.com/science/article/pii/S0166093423001568},
volume = {322},
year = {2023}
}
TY - JOUR
ID - 2353802
AU - Ackermann-Gäumann, Rahel - Brêchet, Arthur - Smetana, Jan - Salát, Jiří - Lienhard, Reto - Croxatto, Antony - Polcarová, Petra - Chlíbek, Roman - Růžek, Daniel
PY - 2023
TI - Vaccination against tick-borne encephalitis elicits a detectable NS1 IgG antibody response
JF - Journal of Virological Methods
VL - 322
IS - December
SP - 1-6
EP - 1-6
PB - Elsevier
SN - 01660934
KW - Tick-borne encephalitis
KW - Antibody
KW - Non-structural protein 1
KW - NS1
KW - Vaccination
KW - Flavivirus
UR - https://www.sciencedirect.com/science/article/pii/S0166093423001568
N2 - Vaccine-induced protection against tick-borne encephalitis virus (TBEV) is mediated by antibodies to the viral particle/envelope protein. The detection of non-structural protein 1 (NS1) specific antibodies has been suggested as a marker indicative of natural infections. However, recent work has shown that TBEV vaccines contain traces of NS1, and immunization of mice induced low amounts of NS1-specific antibodies. In this study, we investigated if vaccination induces TBEV NS1-specific antibodies in humans. Healthy army members (n = 898) were asked to fill in a questionnaire relating to flavivirus vaccination or infection, and blood samples were collected. In addition, samples of 71 suspected acute TBE cases were included. All samples were screened for the presence of TBEV NS1-specific IgG antibodies using an in-house developed ELISA. Antibodies were quantified as percent positivity in reference to a positive control. For qualitative evaluation, cut-off for positivity was defined based on the mean OD of the lower 95% of the vaccinated individuals + 3 SD. We found significantly higher NS1-specific IgG antibody titers (i.e., quantitative evaluation) in individuals having received 2, 3, or 4 or more vaccine doses than in non-vaccinated individuals. Similarly, the percentage of individuals with a positive test result (i.e., qualitative evaluation) was higher in individuals vaccinated against tick-borne encephalitis than in unvaccinated study participants. Although NS1-specific IgG titers remained at a relatively low level when compared to TBE patients, a clear distinction was not always possible. Establishing a clear cut-off point in detection systems is critical for NS1-specific antibodies to serve as a marker for distinguishing the immune response after vaccination and infection.
ER -
ACKERMANN-GÄUMANN, Rahel, Arthur BRÊCHET, Jan SMETANA, Jiří SALÁT, Reto LIENHARD, Antony CROXATTO, Petra POLCAROVÁ, Roman CHLÍBEK and Daniel RŮŽEK. Vaccination against tick-borne encephalitis elicits a detectable NS1 IgG antibody response. \textit{Journal of Virological Methods}. Amsterdam: Elsevier, 2023, vol.~322, December, p.~1-6. ISSN~0166-0934. Available from: https://dx.doi.org/10.1016/j.jviromet.2023.114831.
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