J 2024

Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis

SMOLKOVÁ, Denisa, Dana MORAVCOVÁ and Jana LAVICKÁ

Basic information

Original name

Evaluation of solid-phase extraction sorbents for purification of oligosaccharides and glycans derivatized by positively charged labels followed by capillary electrophoretic analysis

Authors

SMOLKOVÁ, Denisa (703 Slovakia, belonging to the institution), Dana MORAVCOVÁ (203 Czech Republic) and Jana LAVICKÁ (203 Czech Republic, guarantor)

Edition

Journal of Separation Science, Weinheim, Wiley-VCH Verlag GmbH, 2024, 1615-9306

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.100 in 2022

Organization unit

Faculty of Science

DOI

UT WoS

001125781200001

Keywords in English

capillary electrophoresis; cationic labeling; glycans; oligosaccharides; solid-phase extraction

Tags

Tags

International impact, Reviewed
Změněno: 1/2/2024 09:36, Mgr. Marie Šípková, DiS.

Abstract

V originále

The sample preparation including labeling and clean-up represents a key analytical step in the analysis of oligosaccharides and glycans by either chromatographic or electrophoretic separation methods. Although the majority of labeling has been performed by neutral and/or negatively charged tags, the introduction of a positive charge into the saccharide molecule can significantly improve the analysis, especially with mass spectrometry detection. In this work, we present the evaluation of five solid-phase extraction sorbents differing in extraction chemistry for the clean-up and concentration of positively labeled maltooligosaccharides from the reaction mixtures. Maltooligosaccharides containing four to seven glucose units were labeled by cationic tags (2-aminoethyl)trimethylammonium chloride and (carboxymethyl)trimethylammonium chloride hydrazide and the extraction conditions were optimized followed by electrophoretic analysis with conductivity detection. The effects of the solid-phase extraction sorbent chemistry, extraction conditions, and sample composition are discussed. All tested sorbents were capable of cleaning up maltooligosaccharides from the reaction mixtures to some extent after optimization of the solid-phase extraction procedure (51.9%-98.9% recovery). The best-rated amide-based sorbent was used to process the sample of N-linked glycans enzymatically released from ribonuclease B.