Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes

LUKSIKOVA, Karolina, Tomas PAVLICA, Marie ALTMANOVA, Jana STUNDLOVA, Sarka PELIKANOVA, Sergey A SIMANOVSKY, Eugene Yu KRYSANOV, Marek JANKASEK, Matyas HIRMAN, Martin REICHARD, Petr RAB and Alexandr SEMBER. Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes. Journal of Fish Biology. HOBOKEN: WILEY, 2023, vol. 103, No 6, p. 1501-1514. ISSN 0022-1112. Available from: https://dx.doi.org/10.1111/jfb.15550.

Basic information

• Original name: Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes
• Authors: LUKSIKOVA, Karolina, Tomas PAVLICA, Marie ALTMANOVA, Jana STUNDLOVA, Sarka PELIKANOVA, Sergey A SIMANOVSKY, Eugene Yu KRYSANOV, Marek JANKASEK, Matyas HIRMAN, Martin REICHARD (203 Czech Republic, belonging to the institution), Petr RAB and Alexandr SEMBER (guarantor).
• Edition: Journal of Fish Biology, HOBOKEN, WILEY, 2023, 0022-1112.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10617 Marine biology, freshwater biology, limnology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 2.000 in 2022
• RIV identification code: RIV/00216224:14310/23:00132740
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1111/jfb.15550
• UT WoS: 001070903200001
• Keywords in English: chromosome fusion; chromosome polymorphism; FISH; RepeatExplorer; repeatome; sex chromosome
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Using African annual killifishes of the genus Nothobranchius from temporary savannah pools with rapid karyotype and sex chromosome evolution, we analysed the chromosomal distribution of telomeric (TTAGGG)(n) repeat and Nfu-SatC satellite DNA (satDNA; isolated from Nothobranchius furzeri) in 15 species across the Nothobranchius killifish phylogeny, and with Fundulosoma thierryi as an out-group. Our fluorescence in situ hybridization experiments revealed that all analysed taxa share the presence of Nfu-SatC repeat but with diverse organization and distribution on chromosomes. Nfu-SatC landscape was similar in conspecific populations of Nothobranchius guentheri and Nothobranchius melanospilus but slightly-to-moderately differed between populations of Nothobranchius pienaari, and between closely related Nothobranchius kuhntae and Nothobranchius orthonotus. Inter-individual variability in Nfu-SatC patterns was found in N. orthonotus and Nothobranchius krysanovi. We revealed mostly no sex-linked patterns of studied repetitive DNA distribution. Only in Nothobranchius brieni, possessing multiple sex chromosomes, Nfu-SatC repeat occupied a substantial portion of the neo-Y chromosome, similarly as formerly found in the XY sex chromosome system of turquoise killifish N. furzeri and its sister species Nothobranchius kadleci-representatives not closely related to N. brieni. All studied species further shared patterns of expected telomeric repeats at the ends of all chromosomes and no additional interstitial telomeric sites. In summary, we revealed (i) the presence of conserved satDNA class in Nothobranchius clades (a rare pattern among ray-finned fishes); (ii) independent trajectories of Nothobranchius sex chromosome differentiation, with recurrent and convergent accumulation of Nfu-SatC on the Y chromosome in some species; and (iii) genus-wide shared tendency to loss of telomeric repeats during interchromosomal rearrangements. Collectively, our findings advance our understanding of genome structure, mechanisms of karyotype reshuffling, and sex chromosome differentiation in Nothobranchius killifishes from the genus-wide perspective.