Conserved satellite DNA motif and lack of interstitial
telomeric sites in highly rearranged African Nothobranchius
killifish karyotypes

J 2023

Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes

LUKSIKOVA, Karolina, Tomas PAVLICA, Marie ALTMANOVA, Jana STUNDLOVA, Sarka PELIKANOVA et. al.

Basic information

Original name

Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes

Authors

Edition

Journal of Fish Biology, HOBOKEN, WILEY, 2023, 0022-1112

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10617 Marine biology, freshwater biology, limnology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.000 in 2022

RIV identification code

RIV/00216224:14310/23:00132740

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/jfb.15550

UT WoS

001070903200001

Keywords in English

chromosome fusion; chromosome polymorphism; FISH; RepeatExplorer; repeatome; sex chromosome

Abstract

V originále

Using African annual killifishes of the genus Nothobranchius from temporary savannah pools with rapid karyotype and sex chromosome evolution, we analysed the chromosomal distribution of telomeric (TTAGGG)(n) repeat and Nfu-SatC satellite DNA (satDNA; isolated from Nothobranchius furzeri) in 15 species across the Nothobranchius killifish phylogeny, and with Fundulosoma thierryi as an out-group. Our fluorescence in situ hybridization experiments revealed that all analysed taxa share the presence of Nfu-SatC repeat but with diverse organization and distribution on chromosomes. Nfu-SatC landscape was similar in conspecific populations of Nothobranchius guentheri and Nothobranchius melanospilus but slightly-to-moderately differed between populations of Nothobranchius pienaari, and between closely related Nothobranchius kuhntae and Nothobranchius orthonotus. Inter-individual variability in Nfu-SatC patterns was found in N. orthonotus and Nothobranchius krysanovi. We revealed mostly no sex-linked patterns of studied repetitive DNA distribution. Only in Nothobranchius brieni, possessing multiple sex chromosomes, Nfu-SatC repeat occupied a substantial portion of the neo-Y chromosome, similarly as formerly found in the XY sex chromosome system of turquoise killifish N. furzeri and its sister species Nothobranchius kadleci-representatives not closely related to N. brieni. All studied species further shared patterns of expected telomeric repeats at the ends of all chromosomes and no additional interstitial telomeric sites. In summary, we revealed (i) the presence of conserved satDNA class in Nothobranchius clades (a rare pattern among ray-finned fishes); (ii) independent trajectories of Nothobranchius sex chromosome differentiation, with recurrent and convergent accumulation of Nfu-SatC on the Y chromosome in some species; and (iii) genus-wide shared tendency to loss of telomeric repeats during interchromosomal rearrangements. Collectively, our findings advance our understanding of genome structure, mechanisms of karyotype reshuffling, and sex chromosome differentiation in Nothobranchius killifishes from the genus-wide perspective.

Citovat

LUKSIKOVA, Karolina, Tomas PAVLICA, Marie ALTMANOVA, Jana STUNDLOVA, Sarka PELIKANOVA, Sergey A SIMANOVSKY, Eugene Yu KRYSANOV, Marek JANKASEK, Matyas HIRMAN, Martin REICHARD, Petr RAB and Alexandr SEMBER. Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes. Journal of Fish Biology. HOBOKEN: WILEY, 2023, vol. 103, No 6, p. 1501-1514. ISSN 0022-1112. Available from: https://dx.doi.org/10.1111/jfb.15550.

@article{2355406,
   author = {Luksikova, Karolina and Pavlica, Tomas and Altmanova, Marie and Stundlova, Jana and Pelikanova, Sarka and Simanovsky, Sergey A and Krysanov, Eugene Yu and Jankasek, Marek and Hirman, Matyas and Reichard, Martin and Rab, Petr and Sember, Alexandr},
   article_location = {HOBOKEN},
   article_number = {6},
   doi = {http://dx.doi.org/10.1111/jfb.15550},
   keywords = {chromosome fusion; chromosome polymorphism; FISH; RepeatExplorer; repeatome; sex chromosome},
   language = {eng},
   issn = {0022-1112},
   journal = {Journal of Fish Biology},
   title = {Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes},
   url = {https://doi.org/10.1111/jfb.15550},
   volume = {103},
   year = {2023}
}

TY  - JOUR
ID  - 2355406
AU  - Luksikova, Karolina - Pavlica, Tomas - Altmanova, Marie - Stundlova, Jana - Pelikanova, Sarka - Simanovsky, Sergey A - Krysanov, Eugene Yu - Jankasek, Marek - Hirman, Matyas - Reichard, Martin - Rab, Petr - Sember, Alexandr
PY  - 2023
TI  - Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes
JF  - Journal of Fish Biology
VL  - 103
IS  - 6
SP  - 1501-1514
EP  - 1501-1514
PB  - WILEY
SN  - 00221112
KW  - chromosome fusion
KW  - chromosome polymorphism
KW  - FISH
KW  - RepeatExplorer
KW  - repeatome
KW  - sex chromosome
UR  - https://doi.org/10.1111/jfb.15550
N2  - Using African annual killifishes of the genus Nothobranchius from temporary savannah pools with rapid karyotype and sex chromosome evolution, we analysed the chromosomal distribution of telomeric (TTAGGG)(n) repeat and Nfu-SatC satellite DNA (satDNA; isolated from Nothobranchius furzeri) in 15 species across the Nothobranchius killifish phylogeny, and with Fundulosoma thierryi as an out-group. Our fluorescence in situ hybridization experiments revealed that all analysed taxa share the presence of Nfu-SatC repeat but with diverse organization and distribution on chromosomes. Nfu-SatC landscape was similar in conspecific populations of Nothobranchius guentheri and Nothobranchius melanospilus but slightly-to-moderately differed between populations of Nothobranchius pienaari, and between closely related Nothobranchius kuhntae and Nothobranchius orthonotus. Inter-individual variability in Nfu-SatC patterns was found in N. orthonotus and Nothobranchius krysanovi. We revealed mostly no sex-linked patterns of studied repetitive DNA distribution. Only in Nothobranchius brieni, possessing multiple sex chromosomes, Nfu-SatC repeat occupied a substantial portion of the neo-Y chromosome, similarly as formerly found in the XY sex chromosome system of turquoise killifish N. furzeri and its sister species Nothobranchius kadleci-representatives not closely related to N. brieni. All studied species further shared patterns of expected telomeric repeats at the ends of all chromosomes and no additional interstitial telomeric sites. In summary, we revealed (i) the presence of conserved satDNA class in Nothobranchius clades (a rare pattern among ray-finned fishes); (ii) independent trajectories of Nothobranchius sex chromosome differentiation, with recurrent and convergent accumulation of Nfu-SatC on the Y chromosome in some species; and (iii) genus-wide shared tendency to loss of telomeric repeats during interchromosomal rearrangements. Collectively, our findings advance our understanding of genome structure, mechanisms of karyotype reshuffling, and sex chromosome differentiation in Nothobranchius killifishes from the genus-wide perspective.
ER  -

LUKSIKOVA, Karolina, Tomas PAVLICA, Marie ALTMANOVA, Jana STUNDLOVA, Sarka PELIKANOVA, Sergey A SIMANOVSKY, Eugene Yu KRYSANOV, Marek JANKASEK, Matyas HIRMAN, Martin REICHARD, Petr RAB and Alexandr SEMBER. Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes. \textit{Journal of Fish Biology}. HOBOKEN: WILEY, 2023, vol.~103, No~6, p.~1501-1514. ISSN~0022-1112. Available from: https://dx.doi.org/10.1111/jfb.15550.

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