RUVBL proteins are involved in plant gametophyte development

J 2023

RUVBL proteins are involved in plant gametophyte development

DVOŘÁK TOMAŠTÍKOVÁ, Eva, Fen YANG, Kristína MLYNÁROVÁ, Said HAFIDH, Šárka SCHOŘOVÁ et. al.

Basic information

Original name

RUVBL proteins are involved in plant gametophyte development

Authors

DVOŘÁK TOMAŠTÍKOVÁ, Eva, Fen YANG, Kristína MLYNÁROVÁ (703 Slovakia, belonging to the institution), Said HAFIDH, Šárka SCHOŘOVÁ (203 Czech Republic, belonging to the institution), Alžbeta KUSOVÁ (703 Slovakia, belonging to the institution), Markéta PERNISOVÁ (203 Czech Republic, belonging to the institution), Tereza PŘEROVSKÁ (203 Czech Republic, belonging to the institution), Božena KLODOVÁ, David HONYS, Jiří FAJKUS (203 Czech Republic, belonging to the institution), Ales PECINKA and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Journal, Wiley, 2023, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.200 in 2022

RIV identification code

RIV/00216224:14310/23:00132782

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/tpj.16136

UT WoS

000940668400001

Keywords in English

DNA-protein crosslinks; Arabidopsis; male gametophyte; female gametophytes; pollen development; transcription; RUVBL proteins

Abstract

V originále

The proper development of male and female gametophytes is critical for successful sexual reproduction and requires a carefully regulated series of events orchestrated by a suite of various proteins. RUVBL1 and RUVBL2, plant orthologues of human Pontin and Reptin, respectively, belong to the evolutionarily highly conserved AAA(+) family linked to a wide range of cellular processes. Previously, we found that RUVBL1 and RUVBL2A mutations are homozygous lethal in Arabidopsis. Here, we report that RUVBL1 and RUVBL2A play roles in reproductive development. We show that mutant plants produce embryo sacs with an abnormal structure or with various numbers of nuclei. Although pollen grains of heterozygous mutant plants exhibit reduced viability and reduced pollen tube growth in vitro, some of the ruvbl pollen tubes are capable of targeting ovules in vivo. Similarly, some ruvbl ovules retain the ability to attract wild-type pollen tubes but fail to develop further. The activity of the RUVBL1 and RUVBL2A promoters was observed in the embryo sac, pollen grains, and tapetum cells and, for RUVBL2A, also in developing ovules. In summary, we show that the RUVBL proteins are essential for the proper development of both male and particularly female gametophytes in Arabidopsis.

Links

GA21-15841S, research and development project

Name: Genová exprese v samčí zárodečné linii: regulační faktory a jejich responsivní elementy. (Acronym: Regulace gen. exprese v pylu.)

Investor: Czech Science Foundation

GX20-01331X, research and development project

Name: Biogeneze a evoluce telomerázy u rostlin

Investor: Czech Science Foundation

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

DVOŘÁK TOMAŠTÍKOVÁ, Eva, Fen YANG, Kristína MLYNÁROVÁ, Said HAFIDH, Šárka SCHOŘOVÁ, Alžbeta KUSOVÁ, Markéta PERNISOVÁ, Tereza PŘEROVSKÁ, Božena KLODOVÁ, David HONYS, Jiří FAJKUS, Ales PECINKA and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. RUVBL proteins are involved in plant gametophyte development. Plant Journal. Wiley, 2023, vol. 114, No 2, p. 325-337. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.16136.

@article{2356223,
   author = {Dvořák Tomaštíková, Eva and Yang, Fen and Mlynárová, Kristína and Hafidh, Said and Schořová, Šárka and Kusová, Alžbeta and Pernisová, Markéta and Přerovská, Tereza and Klodová, Božena and Honys, David and Fajkus, Jiří and Pecinka, Ales and Procházková Schrumpfová, Petra},
   article_number = {2},
   doi = {http://dx.doi.org/10.1111/tpj.16136},
   keywords = {DNA-protein crosslinks; Arabidopsis; male gametophyte; female gametophytes; pollen development; transcription; RUVBL proteins},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {RUVBL proteins are involved in plant gametophyte development},
   url = {https://doi.org/10.1111/tpj.16136},
   volume = {114},
   year = {2023}
}

TY  - JOUR
ID  - 2356223
AU  - Dvořák Tomaštíková, Eva - Yang, Fen - Mlynárová, Kristína - Hafidh, Said - Schořová, Šárka - Kusová, Alžbeta - Pernisová, Markéta - Přerovská, Tereza - Klodová, Božena - Honys, David - Fajkus, Jiří - Pecinka, Ales - Procházková Schrumpfová, Petra
PY  - 2023
TI  - RUVBL proteins are involved in plant gametophyte development
JF  - Plant Journal
VL  - 114
IS  - 2
SP  - 325-337
EP  - 325-337
PB  - Wiley
SN  - 09607412
KW  - DNA-protein crosslinks
KW  - Arabidopsis
KW  - male gametophyte
KW  - female gametophytes
KW  - pollen development
KW  - transcription
KW  - RUVBL proteins
UR  - https://doi.org/10.1111/tpj.16136
N2  - The proper development of male and female gametophytes is critical for successful sexual reproduction and requires a carefully regulated series of events orchestrated by a suite of various proteins. RUVBL1 and RUVBL2, plant orthologues of human Pontin and Reptin, respectively, belong to the evolutionarily highly conserved AAA(+) family linked to a wide range of cellular processes. Previously, we found that RUVBL1 and RUVBL2A mutations are homozygous lethal in Arabidopsis. Here, we report that RUVBL1 and RUVBL2A play roles in reproductive development. We show that mutant plants produce embryo sacs with an abnormal structure or with various numbers of nuclei. Although pollen grains of heterozygous mutant plants exhibit reduced viability and reduced pollen tube growth in vitro, some of the ruvbl pollen tubes are capable of targeting ovules in vivo. Similarly, some ruvbl ovules retain the ability to attract wild-type pollen tubes but fail to develop further. The activity of the RUVBL1 and RUVBL2A promoters was observed in the embryo sac, pollen grains, and tapetum cells and, for RUVBL2A, also in developing ovules. In summary, we show that the RUVBL proteins are essential for the proper development of both male and particularly female gametophytes in Arabidopsis.
ER  -

DVOŘÁK TOMAŠTÍKOVÁ, Eva, Fen YANG, Kristína MLYNÁROVÁ, Said HAFIDH, Šárka SCHOŘOVÁ, Alžbeta KUSOVÁ, Markéta PERNISOVÁ, Tereza PŘEROVSKÁ, Božena KLODOVÁ, David HONYS, Jiří FAJKUS, Ales PECINKA and Petra PROCHÁZKOVÁ SCHRUMPFOVÁ. RUVBL proteins are involved in plant gametophyte development. \textit{Plant Journal}. Wiley, 2023, vol.~114, No~2, p.~325-337. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.16136.

Detailed Information on Publication Record