Characterization of the conserved features of the NSE6 subunit
of the Physcomitrium patens PpSMC5/6 complex

J 2023

Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex

LELKES, Edit, Jitka JEMELKOVÁ, Marcela HOLÁ, Barbora ŠTEFANOVIE, Peter KOLESÁR et. al.

Basic information

Original name

Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex

Name in Czech

Charakterizace konzervovaných vlastností NSE6 podjednotky Physcomitrium patens komplexu PpSMC5/6

Authors

LELKES, Edit (703 Slovakia, belonging to the institution), Jitka JEMELKOVÁ (203 Czech Republic, belonging to the institution), Marcela HOLÁ (203 Czech Republic), Barbora ŠTEFANOVIE (703 Slovakia, belonging to the institution), Peter KOLESÁR (703 Slovakia, belonging to the institution), Radka VÁGNEROVÁ (203 Czech Republic), Eva DVOŘÁK TOMAŠTÍKOVÁ (203 Czech Republic), Aleš PEČINKA (203 Czech Republic), Karel J. ANGELIS (203 Czech Republic) and Jan PALEČEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLANT JOURNAL, ENGLAND, WILEY, 2023, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.200 in 2022

RIV identification code

RIV/00216224:14310/23:00132792

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1111/tpj.16282

UT WoS

000995683100001

Keywords in English

SMC5/6 complex; NSE5/SNI1/SLF1/SIMC1; NSE6/ASAP1/SLF2/KRE29; CANIN domain; DNA damage repair; plant development; Physcomitrium patens

Abstract

V originále

Structural maintenance of chromosomes (SMC) complexes are molecular machines ensuring chromatin organization at higher levels. They play direct roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of long-armed SMC, kleisin, and kleisin-associated subunits. Additional factors, like NSE6 within SMC5/6, bind to SMC core complexes and regulate their activities. In the human HsNSE6/SLF2, we recently identified a new CANIN domain. Here we tracked down its sequence homology to lower plants, selected the bryophyte Physcomitrium patens, and analyzed PpNSE6 protein-protein interactions to explore its conservation in detail. We identified a previously unrecognized core sequence motif conserved from yeasts to humans within the NSE6 CANIN domain. This motif mediates the interaction between NSE6 and its NSE5 partner in yeasts and plants. In addition, the CANIN domain and its preceding PpNSE6 sequences bind both PpSMC5 and PpSMC6 arms. Interestingly, we mapped the PpNSE6-binding site at the PpSMC5 arm right next to the PpNSE2-binding surface. The position of NSE6 at SMC arms suggests its role in the regulation of SMC5/6 dynamics. Consistent with the regulatory role of NSE6 subunits, Ppnse6 mutant lines were viable and sensitive to the DNA-damaging drug bleomycin and lost a large portion of rDNA copies. These moss mutants also exhibited reduced growth and developmental aberrations. Altogether, our data showed the conserved function of the NSE6 subunit and architecture of the SMC5/6 complex across species.

Links

GA20-05095S, research and development project

Name: Úloha komplexu SMC5/6 a jeho interakčních partnerů v opravě poškozené DNA (Acronym: SMC5/6)

Investor: Czech Science Foundation

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/R/1142/2021, interní kód MU

Name: Visualization of the 3D structure of chromatin

Investor: Masaryk University, CAREER RESTART

MUNI/R/1262/2022, interní kód MU

Name: Vztah mezi opravou DNA a transkripcí

Investor: Masaryk University, Crosstalk between DNA repair and transcription, CAREER RESTART

Citovat

LELKES, Edit, Jitka JEMELKOVÁ, Marcela HOLÁ, Barbora ŠTEFANOVIE, Peter KOLESÁR, Radka VÁGNEROVÁ, Eva DVOŘÁK TOMAŠTÍKOVÁ, Aleš PEČINKA, Karel J. ANGELIS and Jan PALEČEK. Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex. PLANT JOURNAL. ENGLAND: WILEY, 2023, vol. 115, No 4, p. 1084-1099. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.16282.

@article{2356460,
   author = {Lelkes, Edit and Jemelková, Jitka and Holá, Marcela and Štefanovie, Barbora and Kolesár, Peter and Vágnerová, Radka and Dvořák Tomaštíková, Eva and Pečinka, Aleš and Angelis, Karel J. and Paleček, Jan},
   article_location = {ENGLAND},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/tpj.16282},
   keywords = {SMC5/6 complex; NSE5/SNI1/SLF1/SIMC1; NSE6/ASAP1/SLF2/KRE29; CANIN domain; DNA damage repair; plant development; Physcomitrium patens},
   language = {eng},
   issn = {0960-7412},
   journal = {PLANT JOURNAL},
   title = {Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/tpj.16282},
   volume = {115},
   year = {2023}
}

TY  - JOUR
ID  - 2356460
AU  - Lelkes, Edit - Jemelková, Jitka - Holá, Marcela - Štefanovie, Barbora - Kolesár, Peter - Vágnerová, Radka - Dvořák Tomaštíková, Eva - Pečinka, Aleš - Angelis, Karel J. - Paleček, Jan
PY  - 2023
TI  - Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex
JF  - PLANT JOURNAL
VL  - 115
IS  - 4
SP  - 1084-1099
EP  - 1084-1099
PB  - WILEY
SN  - 09607412
KW  - SMC5/6 complex
KW  - NSE5/SNI1/SLF1/SIMC1
KW  - NSE6/ASAP1/SLF2/KRE29
KW  - CANIN domain
KW  - DNA damage repair
KW  - plant development
KW  - Physcomitrium patens
UR  - https://onlinelibrary.wiley.com/doi/10.1111/tpj.16282
N2  - Structural maintenance of chromosomes (SMC) complexes are molecular machines ensuring chromatin organization at higher levels. They play direct roles in cohesion, condensation, replication, transcription, and DNA repair. Their cores are composed of long-armed SMC, kleisin, and kleisin-associated subunits. Additional factors, like NSE6 within SMC5/6, bind to SMC core complexes and regulate their activities. In the human HsNSE6/SLF2, we recently identified a new CANIN domain. Here we tracked down its sequence homology to lower plants, selected the bryophyte Physcomitrium patens, and analyzed PpNSE6 protein-protein interactions to explore its conservation in detail. We identified a previously unrecognized core sequence motif conserved from yeasts to humans within the NSE6 CANIN domain. This motif mediates the interaction between NSE6 and its NSE5 partner in yeasts and plants. In addition, the CANIN domain and its preceding PpNSE6 sequences bind both PpSMC5 and PpSMC6 arms. Interestingly, we mapped the PpNSE6-binding site at the PpSMC5 arm right next to the PpNSE2-binding surface. The position of NSE6 at SMC arms suggests its role in the regulation of SMC5/6 dynamics. Consistent with the regulatory role of NSE6 subunits, Ppnse6 mutant lines were viable and sensitive to the DNA-damaging drug bleomycin and lost a large portion of rDNA copies. These moss mutants also exhibited reduced growth and developmental aberrations. Altogether, our data showed the conserved function of the NSE6 subunit and architecture of the SMC5/6 complex across species.
ER  -

LELKES, Edit, Jitka JEMELKOVÁ, Marcela HOLÁ, Barbora ŠTEFANOVIE, Peter KOLESÁR, Radka VÁGNEROVÁ, Eva DVOŘÁK TOMAŠTÍKOVÁ, Aleš PEČINKA, Karel J. ANGELIS and Jan PALEČEK. Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens PpSMC5/6 complex. \textit{PLANT JOURNAL}. ENGLAND: WILEY, 2023, vol.~115, No~4, p.~1084-1099. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.16282.

Detailed Information on Publication Record